ELISA Reagents

Human MMP-9 ELISA Kit is an in vitro enzyme-linked immunosorbent assay kit used for the quantitative determination of Human Matrix Metalloproteinase-9 (MMP-9) in serum, plasma, and cell culture supernatants. Specific antibodies against Human MMP-9 are pre-coated on high-affinity enzyme plates. Standard samples and test samples are added to the wells of the enzyme plate and, after incubation, the MMP-9 present in the samples binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added and incubated to bind. Following another wash, an enzyme conjugate (Streptavidin-HRP) is added and incubated to bind. After washing, a TMB chromogenic substrate is added for color development in the absence of light. The intensity of the color reaction is proportional to the concentration of MMP-9 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm). MMP-9, also known as Gelatinase B, 92 kDa type IV collagenase, 92 kDa gelatinase, or type V collagenase, is a glycosylated proenzyme. MMP-9 degrades components of the extracellular matrix (ECM) with high activity against denatured collagen (gelatin). It cleaves natural collagens of types III, IV, V, and XI, as well as elastin, nidogen-1, and vitronectin. MMP-9 can also cleave various chemokines and growth factors (such as IL-1β, CXCL 8/IL-8, CXCL 7, CXCL 4, CXCL 1, latent transforming growth factor-β, membrane-bound tumor necrosis factor-α, vascular endothelial growth factor, and basic fibroblast growth factor), amyloid precursor protein, substance P, and myelin basic protein. This activity can either increase or decrease the biological activity of soluble factors and can also release them from their association with the ECM. Specification Item Number P162025S / P162025E Specification 48 T / 96 T Detection Range 31.25-2000 pg/mL Detection Method Sandwich ELISA Detection Species Human Detection Time 5 hours Sensitivity 7.44 pg/mL Dilution Linearity 76.7 -105.9 % Recovery Rate 76.1 - 119.2 % Intra-assay Variability 4.6 % Intra-assay Variability 5.1 % Components Component Number Component Name Storage Temperature P162025S P162025E P162025-A Plate 2~8℃ 48 T 96 T P162025-B Standard 2~8℃ 1 tube 2 tubes P162025-C Detection Antibody 2~8℃ 1 tube 2 tubes P162025-D Enzyme Conjugate 2~8℃(Avoid Light) 170 μL 350 μL P162025-E 5x Dilution Buffer 2~8℃ 12 mL 25 mL P162025-F 20x Wash Buffer 2~8℃ 25 mL 50 mL P162025-G Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL P162025-H Stop Solution   Room Temperature 5 mL 10 mL P162025-I Plate Sealers Room Temperature 3 pieces 5 pieces Storage The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year. Table 1 Reagent Storage Table After Initial Use  Material Name Storage Conditions Plate   Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption. Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination. Detection Antibody Use within 48 hours after dissolution, store at 2~8°C to avoid contamination. Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination. 5×Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination. 20×Wash Buffer Store at 2~8°C for 1 month, avoiding contamination. Substrate solution Store at 2~8°C for 1 month, avoiding light exposure. Stop Solution Can be stored at room temperature. Plate sealing film Can be stored at room temperature.                                    Documents: Manuals P162025-EN-Manual.pdf

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Human IL-8 ELISA Kit is an in vitro enzyme-linked immunosorbent assay kit used for the quantitative determination of Human IL-8 (Interleukin-8) in serum and cell culture supernatants. Specific antibodies against Human IL-8 are pre-coated on high-affinity enzyme plates. Standard samples and test samples are added to the wells of the enzyme plate and, after incubation, the IL-8 present in the samples binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added and incubated to bind. Following another wash, an enzyme conjugate (Streptavidin-HRP) is added and incubated to bind. After washing, aTMB chromogenic substrate is added for color development in the absence of light. The intensity of the color reaction is proportional to the concentration of IL-8 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm). IL-8, also known as CXCL8, is produced by macrophages and epithelial cells. It is a member of the α or CXC chemokine family and has a molecular weight of 8-9 kDa. IL-8 is a chemotactic factor that can attract neutrophils, eosinophils, and T cells, but not monocytes. It also plays a role in neutrophil activation and is released from several cell types in response to inflammatory stimuli. Specification Item Number P162022S / P162022E Specification 48 T / 96 T Detection Range 7.81-500 pg/mL Detection Method Sandwich ELISA Detection Species Human Detection Time 5 hours Sensitivity 0.382 pg/mL Dilution Linearity 83.1 - 109.8% Recovery Rate 73.7 - 112.4% Intra-assay Variability 8.4% Intra-assay Variability 7.0% Components Component Number Component Name Storage Temperature P162022S P162022E P162022-A ELISA Plate 2~8℃ 48 T 96 T P162022-B Standard 2~8℃ 1 tube 2 tubes P162022-C Detection Antibody 2~8℃ 60 μL 120 μL P162022-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL P162022-E 5x Dilution Buffer 2~8℃ 12 mL 25 mL P162022-F 20x Wash Buffer 2~8℃ 25 mL 50 mL P162022-G Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL P162022-H Stop Solution Room Temperature 5 mL 10 mL P162022-I Plate Sealers Room Temperature 3 pieces 5 pieces Storage The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year. Table 1 Reagent Storage Table After Initial Use  Material Name Storage Conditions Plate   Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption. Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination. Detection Antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination. Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination. 5×Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination. 20×Wash Buffer Store at 2~8°C for 1 month, avoiding contamination. Substrate solution Store at 2~8°C for 1 month, avoiding light exposure. Stop Solution Can be stored at room temperature. Plate sealing film Can be stored at room temperature.                                    Documents: Manuals P162022-EN-Manual.pdf

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Human CXCL10 ELISA Kit is an in vitro enzyme-linked immunosorbent assay kit used for the quantitative determination of Human CXCL10 (Interferon-γ-induced Protein 10) in serum, plasma, and cell culture supernatants. Specific antibodies against Human CXCL10 are pre-coated on high-affinity enzyme plates. Standard samples and test samples are added to the wells of the enzyme plate and, after incubation, the CXCL10 present in the samples binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody (biotin-labeled) is added and incubated to bind. Following another wash, an enzyme conjugate (HRP-labeled Streptavidin) is added and incubated to bind. After washing, aTMB chromogenic substrate is added for color development in the absence of light. The intensity of the color reaction is proportional to the concentration of CXCL10 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm (primary wavelength) and 630 nm (secondary wavelength). IP-10 was initially identified as an IFN-γ-induced gene in monocytes, fibroblasts, and endothelial cells. The mouse homolog of human IP-10, CRG-2, shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of IP-10 determines its membership in the CXC chemokine subfamily. Specification Item Number P162021S / P162021E Specification 48 T / 96 T Detection Range 15.63-1000 pg/mL Detection Method Sandwich ELISA Detection Species Human Detection Time 4.75 hours Sensitivity 4.460 pg/mL Dilution Linearity 72.2 - 119.4% Recovery Rate 73.3 - 129.6% Intra-assay Variability 9.2% Intra-assay Variability 6.5% Components Component Number Component Name Storage Temperature P162021S P162021E P162021-A ELISA Plate 2~8℃ 48 T 96 T P162021-B Standard 2~8℃ 1 tube 2 tubes P162021-C Detection Antibody 2~8℃ 1 tube 2 tubes P162021-D Enzyme Conjugate 2~8℃(Avoid Light) 150 μL 300 μL P162021-E 5x Dilution Buffer 2~8℃ 12 mL 25 mL P162021-F 20x Wash Buffer 2~8℃ 25 mL 50 mL P162021-G Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL P162021-H Stop Solution Room Temperature 5 mL 10 mL P162021-I Plate Sealers Room Temperature 3 pieces 5 pieces Storage The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year. Table 1 Reagent Storage Table After Initial Use  Material Name Storage Conditions Plate   Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption. Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination. Detection Antibody Use within 48 hours after dissolution, store at 2~8°C to avoid contamination. Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination. 5×Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination. 20×Wash Buffer Store at 2~8°C for 1 month, avoiding contamination. Substrate solution Store at 2~8°C for 1 month, avoiding light exposure. Stop Solution Can be stored at room temperature. Plate Sealers Can be stored at room temperature.                                    Documents: Manuals P162021-EN-Manual.pdf

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Human IL-21 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of IL-21 in serum, plasma, and cell culture supernatants. Specific anti-IL-21 antibodies are pre-coated onto high-affinity enzyme plates. Standards and samples are added to the wells of the enzyme plate, and after incubation, IL-21 present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for binding and incubated, followed by washing, and then the enzyme complex (Streptavidin-HRP) is added for binding and incubated. After washing, a colorimetric substrateTMB is added for color development, avoiding light. The intensity of the color reaction is proportional to the concentration of IL-21 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm). Human Interleukin-21 (IL-21) is a type I cytokine that regulates the functions of T cells, B cells, NK cells, and myeloid cells. IL-21 is produced by activated follicular helper T cells (Tfh), Th17 cells, and NKT cells. IL-21 derived from Tfh cells plays a crucial role in the development of humoral immunity through its autocrine effects on Tfh cells and paracrine effects on immunoglobulin maturation, plasma cell differentiation, and memory B cells. Mature human IL-21 consists of 133 amino acids and shares 63% and 61% sequence identity with mouse and rat IL-21, respectively. IL-21 is closely associated with clinical conditions such as cancer immunotherapy, viral infections, and allergies. It also inhibits skin hypersensitivity reactions by limiting the production of allergen-specific IgE and degranulation of mast cells. Specification Item Number P162019S / P162019E Specification 48 T / 96 T Detection Range 15.63-1000 pg/mL Detection Method Sandwich ELISA Detection Species Human Detection Time 4.5 hours Sensitivity 8.16 pg/mL Dilution Linearity 81 - 117% Recovery Rate 76 - 121% Intra-assay Variability 3.3% Intra-assay Variability 5.0% Components Component Number Component Name Storage Temperature P162019S P162019E P162019-A ELISA Plate 2~8℃ 48 T 96 T P162019-B Standard 2~8℃ 1 tube 2 tubes P162019-C Detection Antibody 2~8℃ 120 μL 240 μL P162019-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL P162019-E 5x Dilution Buffer 2~8℃ 8 mL 15 mL P162019-F 20x Wash Buffer 2~8℃ 25 mL 50 mL P162019-G Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL P162019-H Stop Solution Room Temperature 5 mL 10 mL P162019-I Plate Sealers Room Temperature 3 pieces 5 pieces Storage The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year. Table 1 Reagent Storage Table After Initial Use  Material Name Storage Conditions Plate   Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption. Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination. Detection Antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination. Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination. 5×Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination. 20×Wash Buffer Store at 2~8°C for 1 month, avoiding contamination. Substrate solution Store at 2~8°C for 1 month, avoiding light exposure. Stop Solution Can be stored at room temperature. Plate Sealers Can be stored at room temperature.                                    Documents: Manuals P162019-EN-Manual.pdf

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Human IL-15 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of Human IL-15 in serum, plasma, and cell culture supernatants. Specific anti-IL-15 antibodies are pre-coated onto high-affinity enzyme plates. Standards and samples are added to the wells of the enzyme plate, and after incubation, IL-15 present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for binding and incubated, followed by washing, and then the enzyme complex (Streptavidin-HRP) is added for binding and incubated. After washing, a colorimetric substrateTMB is added for color development, avoiding light. The intensity of the color reaction is proportional to the concentration of IL-15 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm). Interleukin-15 (IL-15), a widely expressed 14 kDa cytokine, plays a crucial role in numerous immune-related diseases. It shares approximately 97% and 73% sequence identity with IL-15 from primates and rodents, respectively. IL-15 is secreted by monocytes/macrophages (and some other cells), especially macrophages following viral infection. With similar biological functions to IL-2, IL-15 stimulates T cell activation and proliferation by sharing the γc receptor subunit with IL-2. The roles of IL-15 in autoimmune processes (such as rheumatoid arthritis) and malignancies (such as adult T cell leukemia) underscore its significance as a key immunoregulatory molecule, garnering considerable attention from researchers. Specification Item Number P162018S / P162018E Specification 48 T / 96 T Detection Range 3.9-250 pg/mL Detection Method Sandwich ELISA Detection Species Human Detection Time 4.5 hours Sensitivity 1.58 pg/mL Dilution Linearity 83 - 124% Recovery Rate 82 - 116% Intra-assay Variability 3.2% Intra-assay Variability 5.8% Components Component Number Component Name Storage Temperature P162018S P162018E P162018-A ELISA Plate 2~8℃ 48 T 96 T P162018-B Standard 2~8℃ 1 tube 2 tubes P162018-C Detection Antibody 2~8℃ 120 μL 240 μL P162018-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL P162018-E 5x Dilution Buffer 2~8℃ 8 mL 15 mL P162018-F 20x Wash Buffer 2~8℃ 25 mL 50 mL P162018-G Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL P162018-H Stop Solution Room Temperature 5 mL 10 mL P162018-I Plate Sealers Room Temperature 3 pieces 5 pieces Storage The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year. Table 1 Reagent Storage Table After First Use  Material Name Storage Conditions Plate   Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption. Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination. Detection Antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination. Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination. 5×Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination. 20×Wash Buffer Store at 2~8°C for 1 month, avoiding contamination. Substrate solution Store at 2~8°C for 1 month, avoiding light exposure. Stop Solution Can be stored at room temperature. Plate Sealers Can be stored at room temperature.                                    Documents: Manuals P162018-EN-Manual.pdf

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Mouse IL-20 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of IL-20 in serum, plasma, and cell culture supernatants. Specific anti-IL-20 antibodies are pre-coated onto high-affinity enzyme plates. Standards and samples are added to the wells of the enzyme plate, and after incubation, IL-20 present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for binding and incubated, followed by washing, and then the enzyme complex (Streptavidin-HRP) is added for binding and incubated. After washing, a colorimetric substrateTMB is added for color development, avoiding light. The intensity of the color reaction is proportional to the concentration of IL-20 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm). IL-20 belongs to the TGF-β superfamily. IL-20 binds two heterodimeric receptor complexes, IL-20 R alpha/IL-20 R beta and IL-22 R/IL-20 R beta. IL-20 is reported to enhances tissue remodeling and wound-healing activities and restores the homeostasis of epithelial layers during infection and inflammatory responses to maintain tissue integrity. Specification Item Number P162017S / P162017E Specification 48 T / 96 T Detection Range 31.25-2000 pg/mL Detection Method Sandwich ELISA Detection Species Mouse Detection Time 4.5 hours Sensitivity 8.22 pg/mL Dilution Linearity 91 - 120% Recovery Rate 91 - 115% Intra-assay Variability 3.7% Intra-assay Variability 6.1% Components Component Number Component Name Storage Temperature P162017S P162017E P162017-A ELISA Plate 2~8℃ 48 T 96 T P162017-B Standard 2~8℃ 1 tube 2 tubes P162017-C Detection Antibody 2~8℃ 60 μL 120 μL P162017-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL P162017-E 5x Dilution Buffer 2~8℃ 8 mL 15 mL P162017-F 20x Wash Buffer 2~8℃ 25 mL 50 mL P162017-G Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL P162017-H Stop Solution Room Temperature 5 mL 10 mL P162017-I Plate Sealers Room Temperature 3 pieces 5 pieces Storage The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year. Table 1 Reagent Storage Table After First Use  Material Name Storage Conditions Plate   Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption. Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination. Detection Antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination. Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination. 5×Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination. 20×Wash Buffer Store at 2~8°C for 1 month, avoiding contamination. Substrate solution Store at 2~8°C for 1 month, avoiding light exposure. Stop Solution Can be stored at room temperature. Plate Sealers Can be stored at room temperature.                                    Documents: Manuals P162017-EN-Manual.pdf

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Mouse IL-12p70 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of IL-12p70 in serum, plasma, and cell culture supernatants. Specific anti-IL-12p70 antibodies are pre-coated onto high-affinity enzyme plates. Standards and samples are added to the wells of the enzyme plate, and after incubation, IL-12p70 present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for binding and incubated, followed by washing, and then the enzyme complex (Streptavidin-HRP) is added for binding and incubated. After washing, a colorimetric substrateTMB is added for color development, avoiding light. The intensity of the color reaction is proportional to the concentration of IL-12p70 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm). Interleukin-12 (IL-12) is a heterodimeric pro-inflammatory cytokine best known for inducing IFN-γ production in T cells and NK cells. IL-12 consists of two disulfide-linked subunits (p35 and p40) that regulate the biosynthesis of IL-12p70. The effects of IL-12 are mediated through its high-affinity receptor that contains two subunits: IL-12Rβ1 and IL-12Rβ2. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells. Specification Item Number P162016S / P162016E Specification 48 T / 96 T Detection Range 31.25-2000 pg/mL Detection Method Sandwich ELISA Detection Species Mouse Detection Time 4.5 hours Sensitivity 7.19 pg/mL Dilution Linearity 96 - 124% Recovery Rate 82 - 122% Intra-assay Variability 3.2% Components Component Number Component Name Storage Temperature P162016S P162016E P162016-A Plate 2~8℃ 48 T 96 T P162016-B Standard 2~8℃ 1 tube 2 tubes P162016-C Detection Antibody 2~8℃ 120 μL 240 μL P162016-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL P162016-E 5× Dilution Buffer 2~8℃ 8 mL 15 mL P162016-F 20× Wash Buffer 2~8℃ 15 mL 30 mL P162016-G Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL P162016-H Stop Solution Room Temperature 5 mL 10 mL P162016-I Plate Sealers Room Temperature 3 pieces 5 pieces Storage The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year. Table 1 Reagent Storage Table After First Use  Material Name Storage Conditions Plate   Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption. Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination. Detection Antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination. Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination. 5×Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination. 20×Wash Buffer Store at 2~8°C for 1 month, avoiding contamination. Substrate solution Store at 2~8°C for 1 month, avoiding light exposure. Stop Solution Can be stored at room temperature. Plate Sealers Can be stored at room temperature.                                    Documents: Manuals P162016-EN-Manual.pdf

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Human BMP-4 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of BMP-4 in serum, plasma, and cell culture supernatants. Specific anti-BMP-4 antibodies are pre-coated onto high-affinity enzyme plates. Standards and samples are added to the wells of the enzyme plate, and after incubation, BMP-4 present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for binding and incubated, followed by washing, and then the enzyme complex (Streptavidin-HRP) is added for binding and incubated. After washing, a colorimetric substrateTMB is added for color development, avoiding light. The intensity of the color reaction is proportional to the concentration of BMP-4 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm). Bone Morphogenetic Protein 4(BMP4) belongs to the TGF-β superfamily, playing essential roles in many developmental processes, including neurogenesis, vascular development, angiogenesis and osteogenesis. BMP-4 induces cartilage and bone formation. Also acts in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction. Specification Item Number P162015S / P162015E Specification 48 T / 96 T Detection Range 15.63-1000 pg/mL Detection Method Sandwich ELISA Detection Time 4.5 hours Sensitivity 11.2 pg/mL Dilution Linearity 86 - 127% Recovery Rate 75 - 126% Intra-assay Variability 4.0% Inter-assay Variability 5.0% Components Component Number Component Name Storage Temperature P162015S P162015E P162015-A Plate 2~8℃ 48 T 96 T P162015-B Standard 2~8℃ 1 tube 2 tubes P162015-C Detection Antibody 2~8℃ 60 μL 120 μL P162015-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL P162015-E Sample Dilution Buffer 2~8℃ 8 mL 15 mL P162015-F Antibody/Enzyme Dilution Buffer 2~8℃ 15 mL 30 mL P162015-G 20x Wash Buffer 2~8℃ 25 mL 50 mL P162015-H Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL P162015-I Stop Solution Room Temperature 5 mL 10 mL P162015-J Plate Sealers Room Temperature 3 pieces 5 pieces Storage The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year. Table 1 Reagent Storage Table After First Use  Material Name Storage Conditions Plate   Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption. Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination. Detection Antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination. Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination. Sample Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination. 20x Wash Buffer Store at 2~8°C for 1 month, avoiding contamination. Antibody/Enzyme Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination. Substrate solution Store at 2~8°C for 1 month, avoiding light exposure. Stop Solution Can be stored at room temperature. Plate Sealers Can be stored at room temperature.                                    Documents: Manuals P162015-EN-Manual.pdf

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Human Bone Morphogenetic Protein-7 (Human BMP-7) is a member of the TGF-β superfamily and is considered one of the most potent synthetic factors for chondrocytes. The growth factor domain of human BMP-7 shares 98% amino acid sequence identity with mouse and rat BMP-7. BMP-7 acts in various organ systems. It promotes new bone formation and the development of renal units, inhibits differentiation of prostate epithelium, and antagonizes epithelial-mesenchymal transition. Bone Morphogenetic Protein (BMP) signaling regulates a series of cellular processes and plays an important role in early embryonic patterning. It is known that BMP-7 expression has a protective effect on renal injury during diabetic nephropathy and disappears in the early stages of diabetic nephropathy progression. Potential clinical applications of BMP-7 include its neuroprotective effects in stroke animal models. The Arcegen Human BMP-7 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitatively measuring BMP-7 in serum, plasma, and cell culture supernatants. Specific anti-BMP-7 antibodies are precoated onto high-affinity enzyme plates. Standard samples and test samples are added to the enzyme plate wells and incubated. BMP-7 in the samples binds to the solid-phase antibodies. After washing away unbound substances, detection antibodies are added and incubated, followed by washing and addition of enzyme conjugate (Streptavidin-HRP) for incubation. After washing, TMB substrate solution is added for color development in the dark. The intensity of the color reaction is directly proportional to the concentration of BMP-7 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at a wavelength of 450 nm (with a reference wavelength of 570 - 630 nm). Specification Item Number P162014S / P162014E Specification 48 T / 96 T Detection Range 62.5-4000 pg/mL Detection Method Sandwich ELISA Detection Time 4.5 hours Sensitivity 44.6 pg/mL Dilution Linearity 86 - 127% Recovery Rate 81 - 126% Intra-assay Variability 3.3% Inter-assay Variability 4.2% Components Component Number Component Name Storage Temperature P162014S P162014E P162014-A ELISA Plate 2~8℃ 48 T 96 T P162014-B Standard 2~8℃ 1 tube 2 tubes P162014-C Detection Antibody 2~8℃ 60 μL 120 μL P162014-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL P162014-E Sample Dilution Buffer 2~8℃ 8 mL 15 mL P162014-F Antibody/Enzyme Dilution Buffer 2~8℃ 15 mL 30 mL P162014-G 20x Wash Buffer 2~8℃ 25 mL 50 mL P162014-H Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL P162014-I Stop Solution Room Temperature 5 mL 10 mL P162014-J Plate Sealant Film Room Temperature 3 pieces 5 pieces Storage The kit can be stored at 2~8℃ or according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working concentration reagents should be used immediately and discarded, and they should not be reused. The shelf life is 1 year. Table 1 Reagent Storage Table After First Use  Component Names Storage Conditions ELISA Plate   Unused strips can be returned to the aluminum foil bag,tightly sealed,and stored at 2-8°C to avoid moisture absorption. Standard Use within 48 hours after dissolution,store at 2-8°C to avoid contamination. Detection Antibody Use within 48 hours after dilution,store at 2~8°C to avoid contamination. Enzyme Conjugate Use within 48 hours after dilution,store at 2~8°C to avoid contamination. Sample Dilution Buffer Store at 2~8°C for 1 month,avoiding contamination. Antibody/Enzyme Dilution Buffer Store at 2~8°C for 1 month,avoiding contamination. 20x Wash Buffer Store at 2~8°C for 1 month,avoiding contamination. Substrate Solution Store at 2~8°C for 1 month,avoiding light exposure. Stop Solution Can be stored at room temperature. Plate Sealant Film Can be stored at room temperature.                                    Documents: Manuals P162014-EN-Manual.pdf

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Interleukin-6 (IL-6) was initially identified as a B cell differentiation factor. It is now known to be a multifunctional cytokine that regulates immune responses, hematopoiesis, acute-phase reactions, and inflammation. It has three receptor binding sites, including one specific receptor binding site for IL-6R and two binding sites for gp130. IL-6 is a multifunctional, α-helical, 22-28 kDa glycoprotein that plays important roles in acute-phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. Human IL-6 shares 39% amino acid sequence identity with mouse and rat IL-6. It is a pleiotropic cytokine that not only affects the immune system but also acts on various physiological events in other biological systems and organs. It can also induce the growth of myeloma and plasma cell tumors. The Arcegen Human IL-6 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitatively measuring Human Interleukin 6 (Human IL-6) in serum and plasma. Specific anti-Human Interleukin 6 antibodies are precoated onto high-affinity enzyme plates. Standard samples and test samples are added to the enzyme plate wells and incubated. Human Interleukin 6 in the samples binds to the solid-phase antibodies. After washing away unbound substances, detection antibodies are added and incubated, followed by washing and addition of enzyme conjugate (Streptavidin-HRP) for incubation. After washing, TMB substrate solution is added for color development in the dark. The intensity of the color reaction is directly proportional to the concentration of Human Interleukin 6 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at a wavelength of 450 nm (with a reference wavelength of 570 - 630 nm). Specification Item Number P162011S / P162011E Specification 48 T / 96 T Detection Range 7.81-500 pg/mL Detection Method Sandwich ELISA Species Detected human Detection Time 4.5 hours Sensitivity 2.53 pg/mL Dilution Linearity 75 - 116% Recovery Rate 75 - 108% Intra-assay Variability 6.1% Inter-assay Variability 7.6% Components Component Number Component Name Storage Temperature P162011S P162011E P162011-A ELISA Plate 2~8℃ 48 T 96 T P162011-B Standard sample 2~8℃ 1 tube 2 tubes P162011-C Detection antibody 2~8℃ 120 μL 240 μL P162011-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL P162011-E 5× dilution buffer 2~8℃ 8 mL 15 mL P162011-F 20× wash buffer 2~8℃ 25 mL 50 mL P162011-G Substrate solution 2~8℃(Avoid Light) 8 mL 15 mL P162011-H Stop solution Room Temperature 5 mL 10 mL P162011-I Plate sealing film Room Temperature 3 pieces 5 pieces Storage The kit can be stored at 2~8°C, or according to the storage conditions of individual components to avoid contamination and repeated freeze-thaw cycles. Diluted reagents prepared to working concentration should be used immediately and discarded; they should not be reused. The shelf life is 1 year. Table 1 Reagent Storage Table After Initial Use  Component Names Storage Conditions ELISA Plate   Unused strips can be returned to the aluminum foil bag,tightly sealed,and stored at 2-8°C to avoid moisture absorption. Standard sample Use within 48 hours after dissolution,store at 2-8°C to avoid contamination. Detection antibody Use within 48 hours after dilution,store at 2~8°C to avoid contamination. Enzyme conjugate Use within 48 hours after dilution,store at 2~8°C to avoid contamination. 5× dilution buffer Store at 2~8°C for 1 month,avoiding contamination 20× wash buffer Store at 2~8°C for 1 month,avoiding contamination Substrate solution Store at 2~8°C for 1 month,avoiding light exposure. Substrate Solution Store at 2~8°C for 1 month,avoiding light exposure. Stop Solution Can be stored at room temperature Plate sealing film Can be stored at room temperature                                    Documents: Manuals P162011-EN-Manual.pdf

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Human Interleukin-10 (Human IL-10), also known as Cytokine Synthesis Inhibitory Factor (CSIF), can inhibit the activation of Th1 cells and the production of related cytokines (such as IL-1β and TNF-α). IL-10 is a 178 amino acid molecule containing two intrachain disulfide bridges and is expressed as a non-covalently associated homodimer with a molecular weight of 36 kDa. Mature human IL-10 shares 72% to 86% amino acid sequence identity with IL-10 from cow, dog, horse, cat, mouse, sheep, pig, and rat. IL-10 can target various leukocytes, attenuating excessive immune responses. It affects three important functions of monocytes/macrophages: antigen presentation, release of immune mediators, and phagocytosis, representing its inhibition of all functions of monocytes/macrophages. Similar to IL-4 and IL-5, IL-10 is also involved in the development of autoimmune diseases. IL-10's presence can be observed in inflammatory bowel disease (IBD); it plays a protective role in chronic liver inflammation. The Arcegen Human IL-10 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay kit used for the quantitative measurement of IL-10 in serum, plasma, and cell culture supernatants. Specific anti-IL-10 antibodies are pre-coated on a high-affinity enzyme-linked immunosorbent assay plate. Standard samples and test samples are added to the wells of the plate, and after incubation, IL-10 present in the samples binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for incubation and binding, followed by washing. Then, a streptavidin-HRP enzyme conjugate is added for incubation and binding. After washing, aTMB color substrate is added for color development in the absence of light. The intensity of the color reaction is directly proportional to the concentration of IL-10 in the sample. The reaction is terminated by adding a stop solution, and the absorbance values are measured at 450 nm wavelength (reference wavelength 570-630 nm). Specification Item Number P162010S/P162010E Specification 48 T / 96 T Detection Range 62.5-4000pg/mL Detection Method Sandwich ELISA Detection Time 4.5 hours Sensitivity 27.5pg/mL Dilution Linearity 79-117% Recovery Rate 80-118% Intra-assay Variability 5.9% Intra-assay Variability 7.1% Components Component Number Component Name Storage Temperature P162004S P162004E P162010-A ELISA Plate 2~8℃ 48 T 96 T P162010-B Standard 2~8℃ 1 tube 2 tubes P162010-C Detection antibody 2~8℃ 120 μL 240 μL P162010-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL P162010-E Sample Dilution Buffer 2~8℃ 8 mL 15 mL P162010-F Antibody/Enzyme Dilution Buffer 2~8℃ 15 mL 30 mL P162010-G 20x Wash Buffer 2~8℃ 25 mL 50 mL P162010-H Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL P162010-I Stop Solution Room Temperature 5 mL 10 mL P162010-J Plate Sealant Film Room Temperature 3 pieces 5 pieces Storage The kit can be stored at 2~8°C or according to the storage conditions specified for each component to avoid contamination and repeated freeze-thaw cycles. Diluted working concentration reagents should be prepared as needed and discarded after use; they should not be reused. The shelf life is 1 year. Table 1 Reagent Storage Table After Initial Use  Component Name Storage Conditions ELISA Plate   Unused strips can be returned to the aluminum foil bag,tightly sealed,and stored at 2-8°C to avoid moisture absorption. Standard Use within 48 hours after dissolution,store at 2-8°C to avoid contamination. Detection Antibody Use within 48 hours after dilution,store at 2~8°C to avoid contamination. Enzyme Conjugate Use within 48 hours after dilution,store at 2~8°C to avoid contamination. Sample Dilution Buffer Store at 2~8°C for 1 month,avoiding contamination. Antibody/Enzyme Dilution Buffer Store at 2~8°C for 1 month,avoiding contamination. 20x Wash Buffer Store at 2~8°C for 1 month,avoiding contamination. Substrate Solution Store at 2~8°C for 1 month,avoiding light exposure. Stop Solution Can be stored at room temperature. Plate Sealant Film Can be stored at room temperature.                                    Documents: Manuals P162010-EN-Manual.pdf

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Mouse interferon-gamma (Mouse IFN-γ) is a soluble homodimeric cytokine and the sole member of the type II interferon family. It is mainly secreted by natural killer (NK) cells and natural killer T (NKT) cells, playing a crucial role in immune function. Interferon-gamma (IFN-γ) is essential for innate and adaptive immunity against viral, bacterial, and parasitic infections. It acts as a vital activator of macrophages and induces the expression of major histocompatibility complex class II (MHC II). Aberrant expression of IFN-γ is associated with many autoimmune and inflammatory diseases. Besides directly inhibiting viral replication, IFN-γ is important for immune stimulation and regulation. It can effectively inhibit cell proliferation and is used clinically to treat various conditions such as primary thrombocythemia, chronic myeloid leukemia, polycythemia vera, and primary myelofibrosis. The Arcegen Mouse IFN-γ ELISA assay kit is an in vitro enzyme-linked immunosorbent assay (ELISA) kit used for quantitative measurement of mouse interferon-gamma (Mouse IFN-γ) in serum and plasma. Specific anti-mouse interferon-gamma antibodies are precoated on a high-affinity enzyme-linked immunosorbent assay (ELISA) plate. Standard samples and test samples are added to the wells of the ELISA plate, and after incubation, mouse interferon-gamma present in the samples binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for incubation, followed by washing. Streptavidin-horseradish peroxidase (HRP) conjugate is then added for incubation. After another washing step, a colorimetric substrateTMB is added for color development. The intensity of the color reaction is proportional to the concentration of mouse interferon-gamma in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength range of 570 - 630 nm). Specification Item Number P162007S / P162007E Specification 48 T / 96 T Detection Range 31.25-2000 pg/mL Detection Method Sandwich ELISA Detection Time 4.5 hours Sensitivity 5.65 pg/mL Dilution Linearity 82 - 120% Recovery Rate 79 - 108% Intra-assay Variability 4.5% Intra-assay Variability 6.4% Components Component Number Component Name Storage Temperature P162007S P162007E P162007-A ELISA Plate 2~8℃ 48 T 96 T P162007-B Standard 2~8℃ 1 tube 2 tubes P162007-C Detection antibody 2~8℃ 120 μL 240 μL P162007-D Enzyme conjugate 2~8℃(Avoid Light) 30 μL 60 μL P162007-E Sample Dilution Buffer 2~8℃ 8 mL 15 mL P162007-F Antibody/Enzyme Dilution Buffer 2~8℃ 15 mL 30 mL P162007-G 20x Wash Buffer 2~8℃ 25 mL 50 mL P162007-H Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL P162007-I Stop Solution Room Temperature 5 mL 10 mL P162007-J Plate Sealant Film Room Temperature 3 pieces 5 pieces Storage The kit can be stored at 2~8°C or according to the storage conditions provided for each component to avoid contamination and repeated freeze-thaw cycles. Diluted working concentration reagents should be used immediately and discarded after use; they should not be reused. The shelf life is 1 year. Table 1 Reagent Storage Table After Initial Use  Component Name Storage Conditions ELISA Plate   Unused strips can be returned to the aluminum foil bag,tightly sealed,and stored at 2-8°C to avoid moisture absorption. Standard Use within 48 hours after dissolution,store at 2-8°C to avoid contamination. Detection Antibody Use within 48 hours after dilution,store at 2~8°C to avoid contamination. Enzyme Conjugate Use within 48 hours after dilution,store at 2~8°C to avoid contamination. Sample Dilution Buffer Store at 2~8°C for 1 month,avoiding contamination. Antibody/Enzyme Dilution Buffer Store at 2~8°C for 1 month,avoiding contamination. 20x Wash Buffer Store at 2~8°C for 1 month,avoiding contamination. Substrate Solution Store at 2~8°C for 1 month,avoiding light exposure. Stop Solution Can be stored at room temperature. Plate Sealant Film Can be stored at room temperature.                                    Documents: Manuals P162007-EN-Manual.pdf

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Tumor necrosis factor-alpha (TNF-α), also known as cachexin or TNFSF1A, is a multifunctional cytokine that plays a central role in inflammation, apoptosis, and immune system development. It is produced by various immune cells, epithelial cells, endothelial cells, and tumor cells. TNF-α exerts its effects through receptors TNFR1 and TNFR2, activating signaling pathways involving caspase 8, transcription factor NF-κB, and kinase JNK. Human TNF-α shares 79% amino acid homology with mouse TNF-α, suggesting that its biological functions may not exhibit significant species specificity. TNF-α has been shown to confer resistance against certain types of infections while paradoxically inducing inflammation in pathological processes. It may also affect glucose uptake and insulin resistance. Additionally, TNF-α plays a critical role in tumor proliferation, migration, invasion, and angiogenesis. The Arcegen Mouse TNF-α ELISA assay kit is an in vitro enzyme-linked immunosorbent assay (ELISA) kit designed to quantitatively measure mouse TNF-α in serum and plasma samples. The assay utilizes high-affinity anti-mouse TNF-α antibodies pre-coated onto an ELISA plate. Standard samples and test samples are added to the plate wells and incubated, allowing mouse TNF-α present in the samples to bind to the solid-phase antibodies. After washing to remove unbound substances, a detection antibody is added for incubation, followed by addition of enzyme conjugate (Streptavidin-HRP). After washing again, a colorimetric substrate (TMB) is added for color development, and the intensity of the color reaction is proportional to the concentration of mouse TNF-α in the samples. The reaction is terminated, and absorbance is measured at 450 nm wavelength (with a reference wavelength of 570-630 nm). Specification Item Number P162006S / P162006E Specification 48 T / 96 T Detection Range 31.25-2000 pg/mL Detection Method Sandwich ELISA Species Detected Mouse Detection Time 4.5 hours Sensitivity 7.82 pg/mL Dilution Linearity 84 - 124% Recovery Rate 81 - 119% Intra-assay Variability 3.8% Intra-assay Variability 5.3% Components Component Number Component Name Storage Temperature P162006S P162006E P162006-A ELISA Plate 2~8℃ 48 T 96 T P162006-B Standard sample 2~8℃ 1 tube 2 tubes P162006-C Detection antibody 2~8℃ 120 μL 240 μL P162006-D Enzyme conjugate 2~8℃(Avoid Light) 30 μL 60 μL P162006-E 5× dilution buffer 2~8℃ 8 mL 15 mL P162006-F 20× wash buffer 2~8℃ 25 mL 50 mL P162006-G Substrate solution 2~8℃(Avoid Light) 8 mL 15 mL P162006-H Stop solution Room Temperature 5 mL 10 mL P162006-I Plate sealing film Room Temperature 3 pieces 5 pieces Storage The kit can be stored at 2~8°C, or according to the storage conditions of individual components to avoid contamination and repeated freeze-thaw cycles. Diluted reagents prepared to working concentration should be used immediately and discarded; they should not be reused. The shelf life is 1 year. Table 1 Reagent Storage Table After Initial Use  Component Name Storage Conditions ELISA Plate   Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption. Standard sample Use within 48 hours after dissolution, store at 2~8°C to avoid contamination. Detection antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination. Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination. 5× dilution buffer Store at 2~8°C for 1 month, avoiding contamination. 20× wash buffer Store at 2~8°C for 1 month, avoiding contamination. Substrate solution Store at 2~8°C for 1 month, avoiding light exposure. Stop solution Can be stored at room temperature. Plate sealing film Can be stored at room temperature.                                    Documents: Manuals P162006-EN-Manual.pdf

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Vascular Endothelial Growth Factor (VEGF) is the most specific regulator of angiogenesis, providing a morphological basis for endothelial cell migration and tumor cell metastasis. As a foundation for tumor stroma and capillary network formation, VEGF can inhibit apoptosis of tumor cells, although the mechanism remains unclear. The Arcegen Mouse VEGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative measurement of Mouse VEGF in mouse serum, plasma, and cell culture supernatants. High-affinity anti-VEGF antibodies are precoated on the enzyme-linked immunosorbent assay plate. Standards and test samples are added to the plate wells, and after incubation, VEGF present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, detection antibodies (biotin-labeled) are added and incubated to bind. After washing, enzyme conjugate (HRP-labeled streptavidin) is added and incubated to bind. Following washing, a colorimetric substrate, TMB, is added for color development in the absence of light. The intensity of the color reaction is directly proportional to the concentration of VEGF in the sample. The reaction is terminated by adding stop solution, and absorbance values are measured at 450 nm as the primary wavelength and 630 nm as the secondary wavelength. Specification Catalog Number P162005S / P162005E Specification 48 T / 96 T Detection Range 6.25~400 pg/mL Detection Method Sandwich ELISA Species Detected Mouse Detection Time 4 hours 30 minutes Sensitivity 0.11 pg/mL Dilution Linearity 96.7~126.9% Recovery Rate 77.1~124% Intra-assay Variability 4.4% Intra-assay Variability 6.33% Components Component Number Component Name Storage Temperature P162005S P162005E P162005-A ELISA Plate 2~8℃ 48 T 96 T P162005-B Standard 2~8℃ 1 tube 2 tubes P162005-C Detection Antibody 2~8℃ 120 μL 240 μL P162005-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL P162005-E 5×Dilution Buffer 1 2~8℃ 12 mL 25 mL P162005-F 5×Dilution Buffer 2 2~8℃ 8 mL 15 mL P162005-G 20×Wash Buffer 2~8℃ 25 mL 50 mL P162005-H Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL P162005-I Stop Solution Room Temperature 5 mL 10 mL P162005-J Plate Sealing Film Room Temperature 3 pieces 5 pieces Storage The kit can be stored at 2~8°C or according to the storage conditions provided for each component to avoid contamination and repeated freeze-thaw cycles. Diluted working concentration reagents should be used immediately and discarded; they should not be reused. The shelf life is 1 year. Table 1 Reagent Storage Table After Initial Use Component Name Storage Conditions ELISA Plate   Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption. Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination. Detection Antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination. Enzyme Conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination. 5×Dilution Buffer 1 Store at 2~8°C for 1 month, avoiding contamination. 5×Dilution Buffer 2 Store at 2~8°C for 1 month, avoiding contamination. 20×Wash Buffer Store at 2~8°C for 1 month, avoiding contamination. Substrate Solution Store at 2~8°C for 1 month, avoiding light exposure. Stop solution Can be stored at room temperature. Plate Sealing Film Can be stored at room temperature.                                    Documents: Manuals P162005-EN-Manual.pdf

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Mouse interleukin 1β (Mouse IL-1β/IL-1F2), as a major member of the IL-1 family, is primarily produced by activated macrophages. It stimulates thymocyte proliferation through induction of IL-2 release, promotes B-cell maturation and proliferation, and activates fibroblast growth factor to stimulate thymocyte proliferation. It can also stimulate synovial cells to release prostaglandins and various collagenases. IL-1β plays a central role in physiological phenomena such as immunity and inflammation, bone remodeling, fever, and carbohydrate metabolism. Aberrant IL-1β signaling drives tumorigenesis by promoting epithelial-to-mesenchymal transition, increasing production of inflammatory cytokines and chemokines, inducing immune suppression and resistance to cell apoptosis, and increasing leukocyte adhesion. IL-1β-induced inflammation-related diseases include diabetes, arthritis, and atherosclerosis. Moreover, abnormal activation of IL-1β is also associated with poor prognosis in most cancer types, including colon cancer, lung cancer, and breast cancer, among others. The Arcegen Mouse IL-1β/IL-1F2 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of Mouse interleukin 1β (Mouse IL-1β/IL-1F2) in serum and plasma. Specific antibodies against Mouse interleukin 1β are pre-coated on a high-affinity ELISA plate. Standard samples and test samples are added to the wells of the ELISA plate, and after incubation, Mouse interleukin 1β present in the samples binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for incubation and binding, followed by washing and addition of enzyme conjugate (Streptavidin-HRP) for incubation and binding. After washing, a colorimetric substrate TMB is added for color development under light-shielding conditions. The intensity of the color reaction is directly proportional to the concentration of Mouse interleukin 1β in the samples. The reaction is terminated by adding stop solution, and the absorbance is measured at 450 nm wavelength (reference wavelength 570~630 nm). Specification Catalog Number P162004S / P162004E Specification 48 T / 96 T Detection Range 15.63~1000 pg/mL Detection Method Sandwich ELISA Detection Time 4.5 hours Sensitivity 11.46 pg/mL Dilution Linearity 89~123% Recovery Rate 78~107% Intra-assay Variability 4.0% Inter-assay Variability 3.9% Components Component Number Component Name Storage Temperature P162004S P162004E P162004-A ELISA Plate 2~8℃ 48 T 96 T P162004-B Standard 2~8℃ 1 tube 2 tubes P162004-C Detection Antibody 2~8℃ 120 μL 240 μL P162004-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL P162004-E Sample Dilution Buffer 2~8℃ 8 mL 15 mL P162004-F Antibody/Enzyme Dilution Buffer 2~8℃ 15 mL 30 mL P162004-G 20x Wash Buffer 2~8℃ 25 mL 50 mL P162004-H Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL P162004-I Stop Solution Room Temperature 5 mL 10 mL P162004-J Plate Sealant Film Room Temperature 3 pieces 5 pieces Shipping and Storage Reagent Kit can be stored at 2~8°C or according to the storage conditions provided for each component to prevent contamination and repeated freeze-thaw cycles. Dilute reagents to working concentrations immediately before use and discard them afterward; they should not be reused. The shelf life is 1 year. Table 1 Reagent Storage Table After Initial Use  Material Name Storage Conditions Enzyme-linked immunosorbent assay (ELISA)    Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption. plate Standard sample Use within 48 hours after dissolution, store at 2~8°C to avoid contamination. Detecting antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination. Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination. Sample diluent Store at 2~8°C for 1 month, avoiding contamination. 20×Wash solution Store at 2~8°C for 1 month, avoiding contamination. Antibody/enzyme diluent Store at 2~8°C for 1 month, avoiding light exposure. Substrate solution Store at 2~8°C for 1 month, avoiding light exposure. Stop solution Can be stored at room temperature. Plate seal film Can be stored at room temperature.                                    Documents: Manuals P162004-EN-Manual.pdf

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Interleukin-6 (IL-6) was initially identified as a B cell differentiation factor and is now known to be a multifunctional cytokine that regulates immune responses, hematopoiesis, acute-phase reactions, and inflammation. It has three receptor binding sites, including one specific receptor IL-6R binding site, and two gp130 binding sites. IL-6 is a pleiotropic, alpha-helical, 22~28 kDa glycoprotein that plays important roles in acute-phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. Human IL-6 shares 39% amino acid sequence identity with mouse and rat IL-6. It is a multifunctional cytokine that not only affects the immune system but also acts on various biological systems and physiological events in various organs, while also inducing the growth of bone marrow and plasma cell tumors. The Arcegen Mouse IL-6 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for the quantitative determination of mouse interleukin-6 (Mouse IL-6) in serum and plasma. Specific antibodies against mouse interleukin-6 are pre-coated on a high-affinity enzyme immunoassay plate. Standard samples and test samples are added to the wells of the plate, and after incubation, the mouse interleukin-6 present in the samples binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added and incubated for binding. After washing, enzyme conjugate (Streptavidin-HRP) is added and incubated for binding. After washing, a color substrate TMB is added for color development in the dark. The intensity of the color reaction is proportional to the concentration of mouse interleukin-6 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570~630 nm). Specification Catalog Number P162003S/P162003E Specification 48 T / 96 T Detection Range 15.63~1000 pg/mL Detection Method ELISA Detection Time 4.5 hours Sensitivity 9.37 pg/mL Dilution Linearity 74~107% Recovery Rate 77~111% Intra-assay Variability 3.1% Inter-assay Variability 5.7% Components Component Number Component Name Storage Temperature P162003S P162003E P162003-A ELISA Plate 2~8℃ 48 T 96 T P162003-B Standard 2~8℃ 1 tube 2 tubes P162003-C Detection Antibody 2~8℃ 120 μL 240 μL P162003-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL P162003-E 5×Dilution Buffer 2~8℃ 8 mL 15 mL P162003-F 20×Wash Buffer 2~8℃ 25 mL 50 mL P162003-G Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL P162003-H Stop Solution Room Temperature 5 mL 10 mL P162003-I Plate Sealant Film Room Temperature 3 pieces 5 pieces Shipping and Storage Reagent Kit can be stored at 2~8°C or according to the storage conditions provided for each component to prevent contamination and repeated freeze-thaw cycles. Dilute reagents to working concentrations immediately before use and discard them afterward; they should not be reused. The shelf life is 1 year. Table 1 Reagent Storage Table After Initial Use  Material Name Storage Conditions Enzyme Plate  Unused strips can be returned to aluminum foil pouch, tightly sealed, and stored at 2~8°C to avoid moisture absorption. Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination. Detection Antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination. Enzyme Conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination. 5×Dilution Buffer Store at 2~8°C for 1 month, avoid contamination 20×Wash Solution Store at 2~8°C for 1 month, avoid contamination Substrate Solution Store at 2~8°C for 1 month, protect from light. Stop Solution Can be stored at room temperature Sealing Film Can be stored at room temperature                                    Documents: Manuals P162003-EN-Manual.pdf

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Human Fibroblast Growth Factor 10 (FGF-10), also known as Keratinocyte Growth Factor 2 (KGF-2), belongs to the FGF family and is a approximately 20 kDa protein with a region rich in serine near its N-terminus. It shares 93% and 96% amino acid sequence identity with mouse and rat FGF-10, respectively. FGF-10 is considered important during embryonic development and also plays a significant role in adipogenesis. FGF-10 is expressed in the pre-second heart field and, together with Fibroblast Growth Factor 8 (FGF-8), promotes the proliferation of cardiac progenitor cells forming the arterial pole of the heart. The Arcegen Human FGF-10 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of FGF-10 in serum, plasma, and cell culture supernatants. Specific anti-FGF-10 antibodies are pre-coated onto high-affinity enzyme plates. Standards and samples are added to the wells of the enzyme plate, and after incubation, FGF-10 present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for binding and incubated, followed by washing, and then the enzyme complex (Streptavidin-HRP) is added for binding and incubated. After washing, a colorimetric substrateTMB is added for color development, avoiding light. The intensity of the color reaction is proportional to the concentration of FGF-10 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570~630 nm). Specification Item Number P162002E / P162002S Specification 48 T / 96 T Detection Range 1~64 ng/mL Detection Method Sandwich ELISA Detection Time 4.5 hours Sensitivity 0.69 ng/mL Dilution Linearity 77~122% Recovery Rate 77~121% Intra-assay Variability 5.7% Inter-assay Variability 9.2% Components Component Number Component Name Storage Temperature P162002E P162002S P162002-A ELISA Plate 2~8℃ 48 T 96 T P162002-B Standard 2~8℃ 1 tube 2 tubes P162002-C Detection Antibody 2~8℃ 60 μL 120 μL P162002-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL P162002-E Sample Dilution Buffer 2~8℃ 8 mL 15 mL P162002-F Antibody/Enzyme Dilution Buffer 2~8℃ 15 mL 30 mL P162002-G 20x Wash Buffer 2~8℃ 25 mL 50 mL P162002-H Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL P162002-I Stop Solution Room Temperature 5 mL 10 mL P162002-J Plate Sealant Film Room Temperature 3 pieces 5 pieces Shipping and Storage The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year. Table 1 Reagent Storage Table After Initial Use  Material Name Storage Conditions ELISA plate  Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption. Standard sample Use within 48 hours after dissolution, store at 2~8°C to avoid contamination. Detecting antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination. Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination. Sample diluent Store at 2~8°C for 1 month, avoiding contamination. 20×Wash solution Store at 2~8°C for 1 month, avoiding contamination. Antibody/enzyme diluent Store at 2~8°C for 1 month, avoiding contamination. Substrate solution Store at 2~8°C for 1 month, avoiding light exposure. Stop solution Can be stored at room temperature. Plate seal film Can be stored at room temperature.                                    Documents: Manuals P162002-EN-Manual.pdf

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