Transmembrane protein
Recombinant Human Cystine/glutamate transporter (SLC7A11) (Active) _ C230783
Recombinant Human Cystine/glutamate transporter (SLC7A11) (Active) Name Catalog No. Size Recombinant Human Cystine/glutamate transporter (SLC7A11) C230783E 20 μg C230783S 100 μg C230783M 100 μg C230783L 1 mg Product Properties Synonyms (Amino acid transport system xc-)(Calcium channel blocker resistance protein CCBR1)(Solute carrier family 7 member 11)(xCT) Source In vitro E.coli expression system AA sequence Accession # Q9UPY5, Met1 - Leu501 MW 58.2KD Tag N-terminal 10xHis-tagged Purity >90% as determined by SDS-PAGE. Formulation Liquid: Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder: Tris/PBS-based buffer, 6% Trehalose. Activity ELISA: Immobilized Human SLC7A11 at 2 μg/mL can bind Anti-SLC7A11 recombinant antibody, the EC50 is 9.452-13.79 ng/mL. Applications ELISA, Kinetics (BLI), Kinetics (SPR), Immunization Dilution Please reconstitute protein in deionized sterile water or PBS to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. Figure MW and purity analysis by discontinuous SDS-PAGE (reduced). ELISA analysis of its binding ability. Immobilized Human SLC7A11 at 2μg/mL can bind Anti-SLC7A11 recombinant antibody, the EC50 is 9.452-13.79 ng/mL. Loaded Anti-Human SLC7A11 Antibody on 96-Flat plate, can bind Human SLC7A11, with an affinity constant of ＜1 pM as determined in BLI assay (Gator Prime). Storage Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Cautions Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
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