Apoptosis & Proliferation Detection

Product description The Annexin V-FITC/PI Apoptosis Detection Kit uses FITC-labeled Annexin V as a probe to detect the onset of early apoptosis. The detection principle is as follows: in normal living cells, phosphatidylserine (PS) is located on the inner side of the cell membrane, but in early apoptotic cells, PS flips from the inner side of the cell membrane to the surface of the cell membrane and is exposed to the extracellular environment. Annexin V, a calcium-dependent phospholipid-binding protein with a molecular weight of 35-36 kDa, has high affinity for PS and can bind to the membrane of early apoptotic cells through the exposed PS on the outer side of the cell membrane.In addition, Propidium Iodide (PI) is provided in this kit to distinguish surviving early cells from necrotic or late apoptotic cells.PI is a nucleic acid dye that does not penetrate through the intact cell membranes of normal or early apoptotic cells, but can penetrate through the cell membranes of late apoptotic and necrotic cells and stain the nuclei red. Therefore, when Annexin V was used in combination with PI, PI was excluded from living cells (Annexin V-/PI-) and early apoptotic cells (Annexin V+/PI-), while late apoptotic and necrotic cells stained double-positively (Annexin V+/PI+) with both FITC and PI. This kit can be used for detection with flow cytometry or fluorescence microscopy. Specifications Components No. C331406E C331406S C331406M Size 20 T 50 T 100 T Shipping and Storage Transportation of ice packs: Store at -20°C in the dark to avoid repeated freezing and thawing, effective for one year. [Note]If multiple reuses are required within a short period, store in the dark at 4°C, effective for six months. Documents: Manuals C331406-EN-Manual.pdf

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Product description Cell Counting Kit-8, abbreviated as CCK-8, is a rapid and highly sensitive assay for cell proliferation and cytotoxicity based on WST-8 (chemical name: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfophenyl)-2H-tetrazolium monosodium salt), which is an upgraded version of MTT. WST-8 is an upgraded product of MTT, the working principle is: in the presence of electronically coupled reagents, it can be reduced by the dehydrogenase enzyme in the mitochondria to generate a highly water-soluble orange-yellow dirty product (formazan). The shade of color is directly proportional to cell proliferation and inversely proportional to cytotoxicity. The OD value is measured at 450 nm using an enzyme marker, indirectly reflecting the number of viable cells.CCK-8 method has a wide range of applications, such as drug screening, cell proliferation assay, cytotoxicity assay, tumor sensitivity assay, and the assessment of biological factor activity. Components Components No. C331301E C331301S C331301M C331301L C331301T Size 100 T 500 T 1000 T 3×1000 T 10×1000 T Shipping and Storage Ice packs shipping. 2~8℃ dry and lightproof storage, valid for one year.-20℃ dry and lightproof storage, valid for two years. Documents: Manuals C331301-EN-Manual.pdf

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