Mouse interferon-gamma (Mouse IFN-γ) is a soluble homodimeric cytokine and the sole member of the type II interferon family. It is mainly secreted by natural killer (NK) cells and natural killer T (NKT) cells, playing a crucial role in immune function. Interferon-gamma (IFN-γ) is essential for innate and adaptive immunity against viral, bacterial, and parasitic infections. It acts as a vital activator of macrophages and induces the expression of major histocompatibility complex class II (MHC II). Aberrant expression of IFN-γ is associated with many autoimmune and inflammatory diseases. Besides directly inhibiting viral replication, IFN-γ is important for immune stimulation and regulation. It can effectively inhibit cell proliferation and is used clinically to treat various conditions such as primary thrombocythemia, chronic myeloid leukemia, polycythemia vera, and primary myelofibrosis.
The Arcegen Mouse IFN-γ ELISA assay kit is an in vitro enzyme-linked immunosorbent assay (ELISA) kit used for quantitative measurement of mouse interferon-gamma (Mouse IFN-γ) in serum and plasma. Specific anti-mouse interferon-gamma antibodies are precoated on a high-affinity enzyme-linked immunosorbent assay (ELISA) plate. Standard samples and test samples are added to the wells of the ELISA plate, and after incubation, mouse interferon-gamma present in the samples binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for incubation, followed by washing. Streptavidin-horseradish peroxidase (HRP) conjugate is then added for incubation. After another washing step, a colorimetric substrateTMB is added for color development. The intensity of the color reaction is proportional to the concentration of mouse interferon-gamma in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength range of 570 - 630 nm).
Specification
|
Item Number
|
P162007S / P162007E |
| Specification |
48 T / 96 T |
| Detection Range |
31.25-2000 pg/mL |
| Detection Method |
Sandwich ELISA |
| Detection Time |
4.5 hours |
| Sensitivity |
5.65 pg/mL |
| Dilution Linearity |
82 - 120% |
| Recovery Rate |
79 - 108% |
| Intra-assay Variability |
4.5% |
| Intra-assay Variability |
6.4% |
Components
| Component Number |
Component Name |
Storage Temperature |
P162007S |
P162007E |
| P162007-A |
ELISA Plate |
2~8℃ |
48 T |
96 T |
| P162007-B |
Standard |
2~8℃ |
1 tube |
2 tubes |
| P162007-C |
Detection antibody |
2~8℃ |
120 μL |
240 μL |
| P162007-D |
Enzyme conjugate |
2~8℃(Avoid Light) |
30 μL |
60 μL |
| P162007-E |
Sample Dilution Buffer |
2~8℃ |
8 mL |
15 mL |
| P162007-F |
Antibody/Enzyme Dilution Buffer |
2~8℃ |
15 mL |
30 mL |
| P162007-G |
20x Wash Buffer |
2~8℃ |
25 mL |
50 mL |
| P162007-H |
Substrate Solution |
2~8℃(Avoid Light) |
8 mL |
15 mL |
| P162007-I |
Stop Solution |
Room Temperature |
5 mL |
10 mL |
| P162007-J |
Plate Sealant Film |
Room Temperature |
3 pieces |
5 pieces |
Storage
The kit can be stored at 2~8°C or according to the storage conditions provided for each component to avoid contamination and repeated freeze-thaw cycles. Diluted working concentration reagents should be used immediately and discarded after use; they should not be reused. The shelf life is 1 year.
Table 1 Reagent Storage Table After Initial Use
| Component Name
|
Storage Conditions |
| ELISA Plate |
Unused strips can be returned to the aluminum foil bag,tightly sealed,and stored at 2-8°C to avoid moisture absorption. |
| Standard |
Use within 48 hours after dissolution,store at 2-8°C to avoid contamination.
|
| Detection Antibody |
Use within 48 hours after dilution,store at 2~8°C to avoid contamination. |
| Enzyme Conjugate |
Use within 48 hours after dilution,store at 2~8°C to avoid contamination. |
| Sample Dilution Buffer |
Store at 2~8°C for 1 month,avoiding contamination. |
| Antibody/Enzyme Dilution Buffer |
Store at 2~8°C for 1 month,avoiding contamination. |
| 20x Wash Buffer |
Store at 2~8°C for 1 month,avoiding contamination. |
| Substrate Solution |
Store at 2~8°C for 1 month,avoiding light exposure. |
| Stop Solution |
Can be stored at room temperature. |
| Plate Sealant Film |
Can be stored at room temperature. |
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