Mouse IL-12p70 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of IL-12p70 in serum, plasma, and cell culture supernatants. Specific anti-IL-12p70 antibodies are pre-coated onto high-affinity enzyme plates. Standards and samples are added to the wells of the enzyme plate, and after incubation, IL-12p70 present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for binding and incubated, followed by washing, and then the enzyme complex (Streptavidin-HRP) is added for binding and incubated. After washing, a colorimetric substrateTMB is added for color development, avoiding light. The intensity of the color reaction is proportional to the concentration of IL-12p70 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm).
Interleukin-12 (IL-12) is a heterodimeric pro-inflammatory cytokine best known for inducing IFN-γ production in T cells and NK cells. IL-12 consists of two disulfide-linked subunits (p35 and p40) that regulate the biosynthesis of IL-12p70. The effects of IL-12 are mediated through its high-affinity receptor that contains two subunits: IL-12Rβ1 and IL-12Rβ2. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.
Specification
|
Item Number
|
P162016S / P162016E |
| Specification |
48 T / 96 T |
| Detection Range |
31.25-2000 pg/mL |
| Detection Method |
Sandwich ELISA |
| Detection Species |
Mouse |
| Detection Time |
4.5 hours |
| Sensitivity |
7.19 pg/mL |
| Dilution Linearity |
96 - 124% |
| Recovery Rate |
82 - 122% |
| Intra-assay Variability |
3.2% |
Components
| Component Number |
Component Name |
Storage Temperature |
P162016S |
P162016E |
| P162016-A |
Plate |
2~8℃ |
48 T |
96 T |
| P162016-B |
Standard |
2~8℃ |
1 tube |
2 tubes |
| P162016-C |
Detection Antibody |
2~8℃ |
120 μL |
240 μL |
| P162016-D |
Enzyme Conjugate |
2~8℃(Avoid Light) |
30 μL |
60 μL |
| P162016-E |
5× Dilution Buffer |
2~8℃ |
8 mL |
15 mL |
| P162016-F |
20× Wash Buffer |
2~8℃ |
15 mL |
30 mL |
| P162016-G |
Substrate Solution |
2~8℃(Avoid Light) |
8 mL |
15 mL |
| P162016-H |
Stop Solution |
Room Temperature |
5 mL |
10 mL |
| P162016-I |
Plate Sealers |
Room Temperature |
3 pieces |
5 pieces |
Storage
The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year.
Table 1 Reagent Storage Table After First Use
| Material Name
|
Storage Conditions |
| Plate |
Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption. |
| Standard |
Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
|
| Detection Antibody |
Use within 48 hours after dilution, store at 2~8°C to avoid contamination. |
| Enzyme conjugate |
Use within 48 hours after dilution, store at 2~8°C to avoid contamination. |
| 5×Dilution Buffer |
Store at 2~8°C for 1 month, avoiding contamination. |
| 20×Wash Buffer |
Store at 2~8°C for 1 month, avoiding contamination. |
| Substrate solution |
Store at 2~8°C for 1 month, avoiding light exposure. |
| Stop Solution |
Can be stored at room temperature. |
| Plate Sealers |
Can be stored at room temperature. |
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