Interleukin-6 (IL-6) was initially identified as a B cell differentiation factor. It is now known to be a multifunctional cytokine that regulates immune responses, hematopoiesis, acute-phase reactions, and inflammation. It has three receptor binding sites, including one specific receptor binding site for IL-6R and two binding sites for gp130. IL-6 is a multifunctional, α-helical, 22-28 kDa glycoprotein that plays important roles in acute-phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. Human IL-6 shares 39% amino acid sequence identity with mouse and rat IL-6. It is a pleiotropic cytokine that not only affects the immune system but also acts on various physiological events in other biological systems and organs. It can also induce the growth of myeloma and plasma cell tumors.
The Arcegen Human IL-6 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitatively measuring Human Interleukin 6 (Human IL-6) in serum and plasma. Specific anti-Human Interleukin 6 antibodies are precoated onto high-affinity enzyme plates. Standard samples and test samples are added to the enzyme plate wells and incubated. Human Interleukin 6 in the samples binds to the solid-phase antibodies. After washing away unbound substances, detection antibodies are added and incubated, followed by washing and addition of enzyme conjugate (Streptavidin-HRP) for incubation. After washing, TMB substrate solution is added for color development in the dark. The intensity of the color reaction is directly proportional to the concentration of Human Interleukin 6 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at a wavelength of 450 nm (with a reference wavelength of 570 - 630 nm).
Specification
|
Item Number
|
P162011S / P162011E |
| Specification |
48 T / 96 T |
| Detection Range |
7.81-500 pg/mL |
| Detection Method |
Sandwich ELISA |
| Species Detected |
human |
| Detection Time |
4.5 hours |
| Sensitivity |
2.53 pg/mL |
| Dilution Linearity |
75 - 116% |
| Recovery Rate |
75 - 108% |
| Intra-assay Variability |
6.1% |
| Inter-assay Variability |
7.6% |
Components
| Component Number |
Component Name |
Storage Temperature |
P162011S |
P162011E |
| P162011-A |
ELISA Plate |
2~8℃ |
48 T |
96 T |
| P162011-B |
Standard sample |
2~8℃ |
1 tube |
2 tubes |
| P162011-C |
Detection antibody |
2~8℃ |
120 μL |
240 μL |
| P162011-D |
Enzyme Conjugate |
2~8℃(Avoid Light) |
30 μL |
60 μL |
| P162011-E |
5× dilution buffer |
2~8℃ |
8 mL |
15 mL |
| P162011-F |
20× wash buffer |
2~8℃ |
25 mL |
50 mL |
| P162011-G |
Substrate solution |
2~8℃(Avoid Light) |
8 mL |
15 mL |
| P162011-H |
Stop solution |
Room Temperature |
5 mL |
10 mL |
| P162011-I |
Plate sealing film |
Room Temperature |
3 pieces |
5 pieces |
Storage
The kit can be stored at 2~8°C, or according to the storage conditions of individual components to avoid contamination and repeated freeze-thaw cycles. Diluted reagents prepared to working concentration should be used immediately and discarded; they should not be reused. The shelf life is 1 year.
Table 1 Reagent Storage Table After Initial Use
| Component Names
|
Storage Conditions |
| ELISA Plate |
Unused strips can be returned to the aluminum foil bag,tightly sealed,and stored at 2-8°C to avoid moisture absorption. |
| Standard sample |
Use within 48 hours after dissolution,store at 2-8°C to avoid contamination.
|
| Detection antibody |
Use within 48 hours after dilution,store at 2~8°C to avoid contamination. |
| Enzyme conjugate |
Use within 48 hours after dilution,store at 2~8°C to avoid contamination. |
| 5× dilution buffer |
Store at 2~8°C for 1 month,avoiding contamination |
| 20× wash buffer |
Store at 2~8°C for 1 month,avoiding contamination |
| Substrate solution |
Store at 2~8°C for 1 month,avoiding light exposure. |
| Substrate Solution |
Store at 2~8°C for 1 month,avoiding light exposure.
|
| Stop Solution |
Can be stored at room temperature |
| Plate sealing film |
Can be stored at room temperature |
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