Mouse interleukin 1β (Mouse IL-1β/IL-1F2), as a major member of the IL-1 family, is primarily produced by activated macrophages. It stimulates thymocyte proliferation through induction of IL-2 release, promotes B-cell maturation and proliferation, and activates fibroblast growth factor to stimulate thymocyte proliferation. It can also stimulate synovial cells to release prostaglandins and various collagenases. IL-1β plays a central role in physiological phenomena such as immunity and inflammation, bone remodeling, fever, and carbohydrate metabolism. Aberrant IL-1β signaling drives tumorigenesis by promoting epithelial-to-mesenchymal transition, increasing production of inflammatory cytokines and chemokines, inducing immune suppression and resistance to cell apoptosis, and increasing leukocyte adhesion. IL-1β-induced inflammation-related diseases include diabetes, arthritis, and atherosclerosis. Moreover, abnormal activation of IL-1β is also associated with poor prognosis in most cancer types, including colon cancer, lung cancer, and breast cancer, among others.
The Arcegen Mouse IL-1β/IL-1F2 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of Mouse interleukin 1β (Mouse IL-1β/IL-1F2) in serum and plasma. Specific antibodies against Mouse interleukin 1β are pre-coated on a high-affinity ELISA plate. Standard samples and test samples are added to the wells of the ELISA plate, and after incubation, Mouse interleukin 1β present in the samples binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for incubation and binding, followed by washing and addition of enzyme conjugate (Streptavidin-HRP) for incubation and binding. After washing, a colorimetric substrate TMB is added for color development under light-shielding conditions. The intensity of the color reaction is directly proportional to the concentration of Mouse interleukin 1β in the samples. The reaction is terminated by adding stop solution, and the absorbance is measured at 450 nm wavelength (reference wavelength 570~630 nm).
Specification
|
Catalog Number
|
P162004S / P162004E |
| Specification |
48 T / 96 T |
| Detection Range |
15.63~1000 pg/mL |
| Detection Method |
Sandwich ELISA |
| Detection Time |
4.5 hours |
| Sensitivity |
11.46 pg/mL |
| Dilution Linearity |
89~123% |
| Recovery Rate |
78~107% |
| Intra-assay Variability |
4.0% |
| Inter-assay Variability |
3.9% |
Components
| Component Number |
Component Name |
Storage Temperature |
P162004S |
P162004E |
| P162004-A |
ELISA Plate |
2~8℃ |
48 T |
96 T |
| P162004-B |
Standard |
2~8℃ |
1 tube |
2 tubes |
| P162004-C |
Detection Antibody |
2~8℃ |
120 μL |
240 μL |
| P162004-D |
Enzyme Conjugate |
2~8℃(Avoid Light) |
30 μL |
60 μL |
| P162004-E |
Sample Dilution Buffer |
2~8℃ |
8 mL |
15 mL |
| P162004-F |
Antibody/Enzyme Dilution Buffer |
2~8℃ |
15 mL |
30 mL |
| P162004-G |
20x Wash Buffer |
2~8℃ |
25 mL |
50 mL |
| P162004-H |
Substrate Solution |
2~8℃(Avoid Light) |
8 mL |
15 mL |
| P162004-I |
Stop Solution |
Room Temperature |
5 mL |
10 mL |
| P162004-J |
Plate Sealant Film |
Room Temperature |
3 pieces |
5 pieces |
Shipping and Storage
Reagent Kit can be stored at 2~8°C or according to the storage conditions provided for each component to prevent contamination and repeated freeze-thaw cycles. Dilute reagents to working concentrations immediately before use and discard them afterward; they should not be reused. The shelf life is 1 year.
Table 1 Reagent Storage Table After Initial Use
| Material Name
|
Storage Conditions |
| Enzyme-linked immunosorbent assay (ELISA) |
Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption. |
| plate Standard sample |
Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
|
| Detecting antibody |
Use within 48 hours after dilution, store at 2~8°C to avoid contamination. |
| Enzyme conjugate |
Use within 48 hours after dilution, store at 2~8°C to avoid contamination. |
| Sample diluent |
Store at 2~8°C for 1 month, avoiding contamination. |
| 20×Wash solution |
Store at 2~8°C for 1 month, avoiding contamination. |
| Antibody/enzyme diluent |
Store at 2~8°C for 1 month, avoiding light exposure. |
| Substrate solution |
Store at 2~8°C for 1 month, avoiding light exposure.
|
| Stop solution |
Can be stored at room temperature. |
| Plate seal film |
Can be stored at room temperature. |
Documents:
