Human Fibroblast Growth Factor 10 (FGF-10), also known as Keratinocyte Growth Factor 2 (KGF-2), belongs to the FGF family and is a approximately 20 kDa protein with a region rich in serine near its N-terminus. It shares 93% and 96% amino acid sequence identity with mouse and rat FGF-10, respectively. FGF-10 is considered important during embryonic development and also plays a significant role in adipogenesis. FGF-10 is expressed in the pre-second heart field and, together with Fibroblast Growth Factor 8 (FGF-8), promotes the proliferation of cardiac progenitor cells forming the arterial pole of the heart.
The Arcegen Human FGF-10 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of FGF-10 in serum, plasma, and cell culture supernatants. Specific anti-FGF-10 antibodies are pre-coated onto high-affinity enzyme plates. Standards and samples are added to the wells of the enzyme plate, and after incubation, FGF-10 present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for binding and incubated, followed by washing, and then the enzyme complex (Streptavidin-HRP) is added for binding and incubated. After washing, a colorimetric substrateTMB is added for color development, avoiding light. The intensity of the color reaction is proportional to the concentration of FGF-10 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570~630 nm).
Specification
|
Item Number
|
P162002E / P162002S |
| Specification |
48 T / 96 T |
| Detection Range |
1~64 ng/mL
|
| Detection Method |
Sandwich ELISA |
| Detection Time |
4.5 hours |
| Sensitivity |
0.69 ng/mL |
| Dilution Linearity |
77~122%
|
| Recovery Rate |
77~121%
|
| Intra-assay Variability |
5.7% |
| Inter-assay Variability |
9.2% |
Components
| Component Number |
Component Name |
Storage Temperature |
P162002E |
P162002S |
|
P162002-A
|
ELISA Plate |
2~8℃ |
48 T |
96 T |
| P162002-B |
Standard |
2~8℃ |
1 tube |
2 tubes |
| P162002-C |
Detection Antibody |
2~8℃ |
60 μL |
120 μL |
| P162002-D |
Enzyme Conjugate |
2~8℃(Avoid Light) |
30 μL |
60 μL |
| P162002-E |
Sample Dilution Buffer |
2~8℃ |
8 mL |
15 mL |
| P162002-F |
Antibody/Enzyme Dilution Buffer |
2~8℃ |
15 mL |
30 mL |
| P162002-G |
20x Wash Buffer |
2~8℃ |
25 mL |
50 mL |
| P162002-H |
Substrate Solution |
2~8℃(Avoid Light) |
8 mL |
15 mL |
| P162002-I |
Stop Solution |
Room Temperature |
5 mL |
10 mL |
| P162002-J |
Plate Sealant Film |
Room Temperature |
3 pieces |
5 pieces |
Shipping and Storage
The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year.
Table 1 Reagent Storage Table After Initial Use
| Material Name
|
Storage Conditions |
|
ELISA plate
|
Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption. |
| Standard sample |
Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
|
| Detecting antibody |
Use within 48 hours after dilution, store at 2~8°C to avoid contamination. |
| Enzyme conjugate |
Use within 48 hours after dilution, store at 2~8°C to avoid contamination. |
| Sample diluent |
Store at 2~8°C for 1 month, avoiding contamination. |
| 20×Wash solution |
Store at 2~8°C for 1 month, avoiding contamination. |
| Antibody/enzyme diluent |
Store at 2~8°C for 1 month, avoiding contamination. |
| Substrate solution |
Store at 2~8°C for 1 month, avoiding light exposure. |
| Stop solution |
Can be stored at room temperature. |
| Plate seal film |
Can be stored at room temperature. |
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