Human Interleukin-10 (Human IL-10), also known as Cytokine Synthesis Inhibitory Factor (CSIF), can inhibit the activation of Th1 cells and the production of related cytokines (such as IL-1β and TNF-α). IL-10 is a 178 amino acid molecule containing two intrachain disulfide bridges and is expressed as a non-covalently associated homodimer with a molecular weight of 36 kDa. Mature human IL-10 shares 72% to 86% amino acid sequence identity with IL-10 from cow, dog, horse, cat, mouse, sheep, pig, and rat. IL-10 can target various leukocytes, attenuating excessive immune responses. It affects three important functions of monocytes/macrophages: antigen presentation, release of immune mediators, and phagocytosis, representing its inhibition of all functions of monocytes/macrophages. Similar to IL-4 and IL-5, IL-10 is also involved in the development of autoimmune diseases. IL-10's presence can be observed in inflammatory bowel disease (IBD); it plays a protective role in chronic liver inflammation.
The Arcegen Human IL-10 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay kit used for the quantitative measurement of IL-10 in serum, plasma, and cell culture supernatants. Specific anti-IL-10 antibodies are pre-coated on a high-affinity enzyme-linked immunosorbent assay plate. Standard samples and test samples are added to the wells of the plate, and after incubation, IL-10 present in the samples binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for incubation and binding, followed by washing. Then, a streptavidin-HRP enzyme conjugate is added for incubation and binding. After washing, aTMB color substrate is added for color development in the absence of light. The intensity of the color reaction is directly proportional to the concentration of IL-10 in the sample. The reaction is terminated by adding a stop solution, and the absorbance values are measured at 450 nm wavelength (reference wavelength 570-630 nm).
Specification
|
Item Number
|
P162010S/P162010E |
| Specification |
48 T / 96 T |
| Detection Range |
62.5-4000pg/mL |
| Detection Method |
Sandwich ELISA |
| Detection Time |
4.5 hours |
| Sensitivity |
27.5pg/mL |
| Dilution Linearity |
79-117% |
| Recovery Rate |
80-118% |
| Intra-assay Variability |
5.9% |
| Intra-assay Variability |
7.1% |
Components
| Component Number |
Component Name |
Storage Temperature |
P162004S |
P162004E |
| P162010-A |
ELISA Plate |
2~8℃ |
48 T |
96 T |
| P162010-B |
Standard |
2~8℃ |
1 tube |
2 tubes |
| P162010-C |
Detection antibody |
2~8℃ |
120 μL |
240 μL |
| P162010-D |
Enzyme Conjugate |
2~8℃(Avoid Light) |
30 μL |
60 μL |
| P162010-E |
Sample Dilution Buffer |
2~8℃ |
8 mL |
15 mL |
| P162010-F |
Antibody/Enzyme Dilution Buffer |
2~8℃ |
15 mL |
30 mL |
| P162010-G |
20x Wash Buffer |
2~8℃ |
25 mL |
50 mL |
| P162010-H |
Substrate Solution |
2~8℃(Avoid Light) |
8 mL |
15 mL |
| P162010-I |
Stop Solution |
Room Temperature |
5 mL |
10 mL |
| P162010-J |
Plate Sealant Film |
Room Temperature |
3 pieces |
5 pieces |
Storage
The kit can be stored at 2~8°C or according to the storage conditions specified for each component to avoid contamination and repeated freeze-thaw cycles. Diluted working concentration reagents should be prepared as needed and discarded after use; they should not be reused. The shelf life is 1 year.
Table 1 Reagent Storage Table After Initial Use
| Component Name
|
Storage Conditions |
| ELISA Plate |
Unused strips can be returned to the aluminum foil bag,tightly sealed,and stored at 2-8°C to avoid moisture absorption. |
| Standard |
Use within 48 hours after dissolution,store at 2-8°C to avoid contamination.
|
| Detection Antibody |
Use within 48 hours after dilution,store at 2~8°C to avoid contamination. |
| Enzyme Conjugate |
Use within 48 hours after dilution,store at 2~8°C to avoid contamination. |
| Sample Dilution Buffer |
Store at 2~8°C for 1 month,avoiding contamination. |
| Antibody/Enzyme Dilution Buffer |
Store at 2~8°C for 1 month,avoiding contamination. |
| 20x Wash Buffer |
Store at 2~8°C for 1 month,avoiding contamination. |
| Substrate Solution |
Store at 2~8°C for 1 month,avoiding light exposure.
|
| Stop Solution |
Can be stored at room temperature. |
| Plate Sealant Film |
Can be stored at room temperature. |
Documents:
