Human MMP-9 ELISA Kit is an in vitro enzyme-linked immunosorbent assay kit used for the quantitative determination of Human Matrix Metalloproteinase-9 (MMP-9) in serum, plasma, and cell culture supernatants. Specific antibodies against Human MMP-9 are pre-coated on high-affinity enzyme plates. Standard samples and test samples are added to the wells of the enzyme plate and, after incubation, the MMP-9 present in the samples binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added and incubated to bind. Following another wash, an enzyme conjugate (Streptavidin-HRP) is added and incubated to bind. After washing, a TMB chromogenic substrate is added for color development in the absence of light. The intensity of the color reaction is proportional to the concentration of MMP-9 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm).
MMP-9, also known as Gelatinase B, 92 kDa type IV collagenase, 92 kDa gelatinase, or type V collagenase, is a glycosylated proenzyme. MMP-9 degrades components of the extracellular matrix (ECM) with high activity against denatured collagen (gelatin). It cleaves natural collagens of types III, IV, V, and XI, as well as elastin, nidogen-1, and vitronectin. MMP-9 can also cleave various chemokines and growth factors (such as IL-1β, CXCL 8/IL-8, CXCL 7, CXCL 4, CXCL 1, latent transforming growth factor-β, membrane-bound tumor necrosis factor-α, vascular endothelial growth factor, and basic fibroblast growth factor), amyloid precursor protein, substance P, and myelin basic protein. This activity can either increase or decrease the biological activity of soluble factors and can also release them from their association with the ECM.
Specification
|
Item Number
|
P162025S / P162025E |
| Specification |
48 T / 96 T |
| Detection Range |
31.25-2000 pg/mL
|
| Detection Method |
Sandwich ELISA |
| Detection Species |
Human |
| Detection Time |
5 hours
|
| Sensitivity |
7.44 pg/mL
|
| Dilution Linearity |
76.7 -105.9 %
|
| Recovery Rate |
76.1 - 119.2 %
|
| Intra-assay Variability |
4.6 %
|
| Intra-assay Variability |
5.1 %
|
Components
| Component Number |
Component Name |
Storage Temperature |
P162025S |
P162025E |
| P162025-A |
Plate |
2~8℃ |
48 T |
96 T |
| P162025-B |
Standard |
2~8℃ |
1 tube |
2 tubes |
| P162025-C |
Detection Antibody |
2~8℃ |
1 tube |
2 tubes |
| P162025-D |
Enzyme Conjugate |
2~8℃(Avoid Light) |
170 μL |
350 μL |
| P162025-E |
5x Dilution Buffer |
2~8℃ |
12 mL |
25 mL |
| P162025-F |
20x Wash Buffer
|
2~8℃ |
25 mL |
50 mL |
| P162025-G |
Substrate Solution
|
2~8℃(Avoid Light) |
8 mL |
15 mL |
| P162025-H |
Stop Solution |
Room Temperature
|
5 mL |
10 mL |
| P162025-I |
Plate Sealers
|
Room Temperature |
3 pieces
|
5 pieces
|
Storage
The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year.
Table 1 Reagent Storage Table After Initial Use
| Material Name
|
Storage Conditions |
| Plate |
Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption.
|
| Standard |
Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
|
| Detection Antibody |
Use within 48 hours after dissolution, store at 2~8°C to avoid contamination. |
| Enzyme conjugate |
Use within 48 hours after dilution, store at 2~8°C to avoid contamination. |
| 5×Dilution Buffer |
Store at 2~8°C for 1 month, avoiding contamination. |
| 20×Wash Buffer |
Store at 2~8°C for 1 month, avoiding contamination. |
| Substrate solution |
Store at 2~8°C for 1 month, avoiding light exposure.
|
| Stop Solution |
Can be stored at room temperature. |
| Plate sealing film |
Can be stored at room temperature. |
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