Vascular Endothelial Growth Factor (VEGF) is the most specific regulator of angiogenesis, providing a morphological basis for endothelial cell migration and tumor cell metastasis. As a foundation for tumor stroma and capillary network formation, VEGF can inhibit apoptosis of tumor cells, although the mechanism remains unclear.
The Arcegen Mouse VEGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative measurement of Mouse VEGF in mouse serum, plasma, and cell culture supernatants. High-affinity anti-VEGF antibodies are precoated on the enzyme-linked immunosorbent assay plate. Standards and test samples are added to the plate wells, and after incubation, VEGF present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, detection antibodies (biotin-labeled) are added and incubated to bind. After washing, enzyme conjugate (HRP-labeled streptavidin) is added and incubated to bind. Following washing, a colorimetric substrate, TMB, is added for color development in the absence of light. The intensity of the color reaction is directly proportional to the concentration of VEGF in the sample. The reaction is terminated by adding stop solution, and absorbance values are measured at 450 nm as the primary wavelength and 630 nm as the secondary wavelength.
Specification
|
Catalog Number
|
P162005S / P162005E |
| Specification |
48 T / 96 T |
| Detection Range |
6.25~400 pg/mL |
| Detection Method |
Sandwich ELISA |
| Species Detected |
Mouse |
| Detection Time |
4 hours 30 minutes |
| Sensitivity |
0.11 pg/mL |
| Dilution Linearity |
96.7~126.9% |
| Recovery Rate |
77.1~124% |
| Intra-assay Variability |
4.4% |
| Intra-assay Variability |
6.33% |
Components
| Component Number |
Component Name |
Storage Temperature |
P162005S |
P162005E |
| P162005-A |
ELISA Plate |
2~8℃ |
48 T |
96 T |
| P162005-B |
Standard |
2~8℃ |
1 tube |
2 tubes |
| P162005-C |
Detection Antibody |
2~8℃ |
120 μL |
240 μL |
| P162005-D |
Enzyme Conjugate |
2~8℃(Avoid Light) |
30 μL |
60 μL |
| P162005-E |
5×Dilution Buffer 1 |
2~8℃ |
12 mL |
25 mL |
| P162005-F |
5×Dilution Buffer 2 |
2~8℃ |
8 mL |
15 mL |
| P162005-G |
20×Wash Buffer |
2~8℃ |
25 mL |
50 mL |
| P162005-H |
Substrate Solution |
2~8℃(Avoid Light) |
8 mL |
15 mL |
| P162005-I |
Stop Solution |
Room Temperature |
5 mL |
10 mL |
| P162005-J |
Plate Sealing Film |
Room Temperature |
3 pieces |
5 pieces |
Storage
The kit can be stored at 2~8°C or according to the storage conditions provided for each component to avoid contamination and repeated freeze-thaw cycles. Diluted working concentration reagents should be used immediately and discarded; they should not be reused. The shelf life is 1 year.
Table 1 Reagent Storage Table After Initial Use
|
Component Name
|
Storage Conditions |
| ELISA Plate |
Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption. |
| Standard |
Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
|
| Detection Antibody |
Use within 48 hours after dilution, store at 2~8°C to avoid contamination. |
| Enzyme Conjugate |
Use within 48 hours after dilution, store at 2~8°C to avoid contamination. |
| 5×Dilution Buffer 1 |
Store at 2~8°C for 1 month, avoiding contamination. |
| 5×Dilution Buffer 2 |
Store at 2~8°C for 1 month, avoiding contamination. |
| 20×Wash Buffer |
Store at 2~8°C for 1 month, avoiding contamination. |
| Substrate Solution |
Store at 2~8°C for 1 month, avoiding light exposure. |
| Stop solution |
Can be stored at room temperature. |
| Plate Sealing Film |
Can be stored at room temperature. |
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