Interleukin-6 (IL-6) was initially identified as a B cell differentiation factor and is now known to be a multifunctional cytokine that regulates immune responses, hematopoiesis, acute-phase reactions, and inflammation. It has three receptor binding sites, including one specific receptor IL-6R binding site, and two gp130 binding sites. IL-6 is a pleiotropic, alpha-helical, 22~28 kDa glycoprotein that plays important roles in acute-phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. Human IL-6 shares 39% amino acid sequence identity with mouse and rat IL-6. It is a multifunctional cytokine that not only affects the immune system but also acts on various biological systems and physiological events in various organs, while also inducing the growth of bone marrow and plasma cell tumors.
The Arcegen Mouse IL-6 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for the quantitative determination of mouse interleukin-6 (Mouse IL-6) in serum and plasma. Specific antibodies against mouse interleukin-6 are pre-coated on a high-affinity enzyme immunoassay plate. Standard samples and test samples are added to the wells of the plate, and after incubation, the mouse interleukin-6 present in the samples binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added and incubated for binding. After washing, enzyme conjugate (Streptavidin-HRP) is added and incubated for binding. After washing, a color substrate TMB is added for color development in the dark. The intensity of the color reaction is proportional to the concentration of mouse interleukin-6 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570~630 nm).
Specification
|
Catalog Number
|
P162003S/P162003E |
| Specification |
48 T / 96 T |
| Detection Range |
15.63~1000 pg/mL |
| Detection Method |
ELISA |
| Detection Time |
4.5 hours |
| Sensitivity |
9.37 pg/mL |
| Dilution Linearity |
74~107% |
| Recovery Rate |
77~111% |
| Intra-assay Variability |
3.1% |
| Inter-assay Variability |
5.7% |
Components
| Component Number |
Component Name |
Storage Temperature |
P162003S |
P162003E |
| P162003-A |
ELISA Plate |
2~8℃ |
48 T |
96 T |
| P162003-B |
Standard |
2~8℃ |
1 tube |
2 tubes |
| P162003-C |
Detection Antibody |
2~8℃ |
120 μL |
240 μL |
| P162003-D |
Enzyme Conjugate |
2~8℃(Avoid Light) |
30 μL |
60 μL |
| P162003-E |
5×Dilution Buffer |
2~8℃ |
8 mL |
15 mL |
| P162003-F |
20×Wash Buffer |
2~8℃ |
25 mL |
50 mL |
| P162003-G |
Substrate Solution |
2~8℃(Avoid Light) |
8 mL |
15 mL |
| P162003-H |
Stop Solution |
Room Temperature |
5 mL |
10 mL |
| P162003-I |
Plate Sealant Film |
Room Temperature |
3 pieces |
5 pieces |
Shipping and Storage
Reagent Kit can be stored at 2~8°C or according to the storage conditions provided for each component to prevent contamination and repeated freeze-thaw cycles. Dilute reagents to working concentrations immediately before use and discard them afterward; they should not be reused. The shelf life is 1 year.
Table 1 Reagent Storage Table After Initial Use
| Material Name
|
Storage Conditions |
| Enzyme Plate |
Unused strips can be returned to aluminum foil pouch, tightly sealed, and stored at 2~8°C to avoid moisture absorption. |
| Standard |
Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
|
| Detection Antibody |
Use within 48 hours after dilution, store at 2~8°C to avoid contamination. |
| Enzyme Conjugate |
Use within 48 hours after dilution, store at 2~8°C to avoid contamination. |
| 5×Dilution Buffer |
Store at 2~8°C for 1 month, avoid contamination |
| 20×Wash Solution |
Store at 2~8°C for 1 month, avoid contamination |
| Substrate Solution |
Store at 2~8°C for 1 month, protect from light. |
| Stop Solution |
Can be stored at room temperature |
| Sealing Film |
Can be stored at room temperature |
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