Products

Product description 3DCultr Breast Cancer Organoid Growth Medium (Human) is a serum-free medium suitable for the establishment and long-term culture of breast cancer organoids derived from cells or tissues. Under conditions where extracellular matrix is present, this medium contains unique components and abundant cytokines that promote the rapid growth and formation of breast cancer cells into breast cancer organoids. The process of organoid formation is smooth and rapid, while maintaining high characteristics and vitality of breast cancer cells. This medium supports subsequent research in physiology, disease, and precision medicine based on breast cancer organoids. Specifications Catalog Number C231109E/C231109S/C231109M Specifications 50 mL/100 mL/500 mL Components Component Number Component Name C231109E C231109S C231109M C231109-A Intestinal Cancer Organoid Growth Medium 45 mL 90 mL 450 mL C231109-B Nutritional components (10×) 5 mL 10 mL 50 mL Storage Stored at -25℃~-15℃, valid for 1 year; when stored at 2~8℃, valid for 1 month. Documents: Manuals C231109-EN-Manual.pdf

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3DCultr Cell freezing medium for Organoid is a serum-free organoid freezing medium with independent intellectual property rights and a unique formula. It features a proprietary formulation with a clear chemical composition, free from bovine serum or other protein components. With its high efficiency, low toxicity, absence of exogenous factors, and ease of use, it is recommended for the cryopreservation of various types of organoids. Specification Catalog Number C231104E/C231104S Specification 30 mL/100 mL Components Component Number Component Name C231104E C231104S C231104 3DCultr Cell freezing medium for Organoid 30 mL 100 mL Storage Store at 2~8℃, use aseptically, and has a validity period of 12 months; store at -20℃, and have a validity period of 24 months. Documents: Manuals C231104-EN-Manual.pdf

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3DCultr Esophageal Cancer Organoid Growth Medium (Human) is a serum-free medium that can be used for the establishment and long-term culture of Esophageal Cancer organoids derived from cells or tissues. In the presence of extracellular matrix, The unique components and abundant cytokines contained in the culture medium can promote the rapid growth and formation of Esophageal Cancer organoids from Esophageal Cancer cells. The organoid formation process is smooth and rapid, while maintaining high characteristics and vitality of Esophageal Cancer cells, This provides support for subsequent physiological function studies, disease research, and precision medicine based on esophageal cancer organoids. Specification Product Name Cat# Size 3DCultr Esophageal Cancer Organoid Growth Medium (Human) C231111E 50 mL 3DCultr Esophageal Cancer Organoid Growth Medium (Human) C231111S 100 mL 3DCultr Esophageal Cancer Organoid Growth Medium (Human) C231111M 500 mL Components Contents No. Contents Name Catalog No./Specification C231111-A Esophageal Cancer Organoid Growth Medium(Human) 45 mL 90 mL 450 mL C231111-B Nutritional components 1(10×) 5 mL 10 mL 50 mL Storage stored at -25℃ ~-15℃, the validity period is 1 year; when stored at 2~8℃, the validity period is 1 month. Documents: Manuals C231111-EN-Manual.pdf

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Product description 3DCultr Gastric Cancer Organoid Growth Medium (Human) is a serum-free medium that can be used for the establishment and long-term culture of gastric cancer organoids derived from cells or tissues. In the presence of extracellular matrix, The unique components and abundant cytokines contained in the culture medium can promote the rapid growth and formation of gastric cancer organoids from gastric cancer cells. The organoid formation process is smooth and rapid, while maintaining high characteristics and vitality of gastric cancer cells, which provides a basis for subsequent support for physiological functions, disease research and precision medicine of gastric cancer organoids. Specifications Catalog Number C231107E/C231107S/C231107M Specifications 50 mL/100 mL/500 mL Components Component Number Component Name C231107E C231107S C231107M C231107-A Gastric Cancer Organoid Growth Medium 45 mL 90 mL 450 mL C231107-B Nutritional components（10×） 5 mL 10 mL 50 mL Storage Stored at -25℃~-15℃, valid for 1 year; when stored at 2~8℃, valid for 1 month. Documents: Manuals C231107-EN-Manual.pdf

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Product description 3DCultr Intestinal Cancer Organoid Growth Medium (Human) is a serum-free medium that can be used for the establishment and long-term culture of intestinal cancer organoids derived from cells or tissues. In the presence of extracellular matrix, The unique components and abundant cytokines contained in the culture medium can promote the rapid growth and formation of intestinal cancer organoids from intestinal cancer cells. The organoid formation process is smooth and rapid, while maintaining high characteristics and vitality of intestinal cancer cells, which provides a basis for subsequent Provide support for physiological functions, disease research and precision medicine of intestinal cancer organoids. Specifications Catalog Number C231106E/C231106S/C231106M Specifications 50 mL/100 mL/500 mL Components Component Number Component Name C231106E C231106S C231106M C231106-A Intestinal Cancer Organoid Growth Medium 45 mL 90 mL 450 mL C231106-B Nutritional components (10×) 5 mL 10 mL 50 mL Storage Stored at -25℃ ~-15℃, the validity period is 1 year; when stored at 2~8℃, the validity period is 1 month. Documents: Manuals C231106-EN-Manual.pdf

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Product description 3DCultr Intestinal Organoid Growth Medium (Mouse) is a serum-free medium that can be used for the establishment and long-term culture of mouse intestinal organoids derived from mouse intestinal stem cells (such as intestinal crypts). In the presence of extracellular matrix, the medium contains specific components and abundant growth factors that promote the rapid growth and formation of intestinal organoids from intestinal stem cells. The process of organoid formation is smooth and rapid, while maintaining high characteristics and vitality of intestinal stem cells. It supports subsequent research on intestinal physiological functions, diseases, and precision medicine based on organoids. Specifications Catalog Number C231105E/C231105S Specifications 100 mL/500 mL Components Component Number Component Name C231105E C231105S C231105-A Intestinal Organoid Growth Basal Medium 80 mL 400 mL C231105-B Nutritional components 1(10×) 10 mL 50 mL C231105-C Nutritional components 2(10×) 10 mL 50 mL Storage Store at -25℃~-15℃, valid for 1 year. Documents: Manuals C231105-EN-Manual.pdf

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Product description 3DCultr Liver Cancer Organoid Growth Medium (Human) is a serum-free medium that can be used for the establishment and long-term culture of liver cancer organoids derived from cells or tissues. Under the conditions of extracellular matrix, this medium contains unique components and abundant cytokines that promote the rapid growth and formation of liver cancer cells into liver cancer organoids.The process of organoid formation is smooth and rapid while maintaining the high characteristics and vitality of liver cancer cells, thereby providing support for subsequent studies on the physiological functions, disease research, and precision medicine based on liver cancer organoids. Specifications Catalog Number C231108E/C231108S/C231108M Specifications 50 mL/100 mL/500 mL Components Component Number Component Name C231108E C231108S C231108M C231108-A Liver Cancer Organoid Growth Medium 45 mL 90 mL 450 mL C231108-B Nutritional components（10×） 5 mL 10 mL 50 mL Storage Stored at -25℃~-15℃, valid for 1 year; when stored at 2~8℃, valid for 1 month. Documents: Manuals C231108-EN-Manual.pdf

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3DCultr Lung Cancer Organoid Growth Medium (Human) is a serum-free medium that can be used for the establishment and long-term culture of lung cancer organoids derived from cells or tissues. In the presence of extracellular matrix, The unique components and abundant cytokines contained in the culture medium can promote the rapid growth and formation of lung cancer organoids from lung cancer cells. The organoid formation process is smooth and rapid, while maintaining high characteristics and vitality of lung cancer cells, which provides a basis for subsequent support for physiological functions, disease research and precision medicine of lung cancer organoids. Specification Catalog Number C231110E/C231110S/C231110M Specification 50 mL/100 mL/500 mL Components Component Number Component Name C231110E C231110S C231110M C231110-A Lung Cancer Organoid Growth Medium 45 mL 90 T 450 mL C231110-B Nutritional components (10×) 5 mL 10 mL 50 mL Storage Stored at -25℃~-15℃, valid for 1 year; when stored at 2~8℃, valid for 1 month.  Documents: Manuals C231110-EN-Manual.pdf

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3DCultr Pancreatic cancer Organoid Growth Medium (Human)is a serum-free medium designed for the establishment and long-term culture of pancreatic cancer organoids derived from cells or tissues. Under conditions where extracellular matrix is present, the medium contains unique components and abundant cytokines that promote the rapid growth and formation of pancreatic cancer cells into pancreatic cancer organoids. The process of organoid formation is smooth and rapid while maintaining high characteristics and vitality of pancreatic cancer cells. This medium supports subsequent studies and precision medicine based on the physiological functions and disease research of pancreatic cancer organoids. Specification Product Name Cat# Size 3DCultr Pancreatic cancer Organoid Growth Medium (Human) C231113E 50 mL C231113S 100 mL C231113M 500 mL Components Contents No. Contents Name Catalog No./Specification C231113-A Pancreatic Cancer Organoid Growth Medium(Human) 45 mL 90 mL 450 mL C231113-B Nutritional components 1(10×) 5 mL 10 mL 50 mL Storage Stored at -20°C, the product has a shelf life of 1 year. When stored at 2~8°C, its shelf life is 1 month. Documents: Manuals C231113-EN-Manual.pdf

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N-2 supplement are chemically defined additives. N-2 supplement is primarily recommended for the growth and expression of anaphase neurons of neuroblastoma and peripheral nervous system (PNS) and central nervous system (CNS) origin. This product is available at 100X working concentration and can be used with serum-free neuronal basal medium or DMEM/F12 medium supplemented with growth factors such as bFGF and EGF.    Product Components Bovine Transferrin(HOLO)  Putrescine  Insulin  Sodium Selenite  Progesterone Storage The products are shipped with ice pack and can be stored at -25~-15 ℃ for 2 year.  Cautions 1. Please operate with lab coats and disposable gloves,for your safety.        2. This product is for research use only.  

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NSC-27, one of the most frequently cited neural cell culture supplements, is a serum-free supplement optimized to support the growth of embryonic, postnatal, and adult hippocampal neurons and other central nervous system (CNS) neurons. The NSC-27 Serum Free Supplement is supplied as a 50X working solution and is designed to be used with Neurobasal Medium, which is used for neuronal cell culture without the need for an astrocyte feeder layer. Use NSC-27 Supplement in Neuronal Basal Medium for culturing prenatal and embryonic neurons for optimized viability and long-term survival. NSC-27 can replace B-27 supplement.   Product Components Catalase T3 Putrescine Linoleic Acid Oleic acid Lpoic acid I-carnitine Putrescine Linolenic Acid Biotin Corticoseterone Ethanolamine Insulin D(+) Galactose Progesterone Pipecolic acid Bovine Transferrin(HOLO) Retinol acetate Storage The products are shipped with ice pack and can be stored at -25~-15℃ for 2 year.  Cautions 1. Please operate with lab coats and disposable gloves,for your safety.        2. This product is for research use only.  

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NSC-27, one of the most frequently cited neural cell culture supplements, is a serum-free supplement optimized to support the growth of embryonic, postnatal, and adult hippocampal neurons and other central nervous system (CNS) neurons. The NSC-27 Serum Free Supplement is supplied as a 50X working solution and is designed to be used with Neurobasal Medium, which is used for neuronal cell culture without the need for an astrocyte feeder layer. Use NSC-27 Supplement in Neuronal Basal Medium for culturing prenatal and embryonic neurons for optimized viability and long-term survival. NSC-27 can replace B-27 supplement.   Product Components Catalase T3 Putrescine Linoleic Acid DL-a-Tocopherol (Vitamin E) Oleic acid Lpoic acid Glutathione I-carnitine Putrescine Linolenic Acid DL-a-Tocopherol Acetate Biotin Superoxide Dismutase Insulin D(+) Galactose Corticoseterone Progesterone Pipecolic acid Bovine Transferrin(HOLO) Ethanolamine Storage The products are shipped with ice pack and can be stored at -25~-15℃ for 2 year.  Cautions 1. Please operate with lab coats and disposable gloves,for your safety.        2. This product is for research use only.  

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NSC-27, one of the most frequently cited neural cell culture supplements, is a serum-free supplement optimized to support the growth of embryonic, postnatal, and adult hippocampal neurons and other central nervous system (CNS) neurons. The NSC-27 Serum Free Supplement is supplied as a 50X working solution and is designed to be used with Neurobasal Medium, which is used for neuronal cell culture without the need for an astrocyte feeder layer. Use NSC-27 Supplement in Neuronal Basal Medium for culturing prenatal and embryonic neurons for optimized viability and long-term survival. NSC-27 can replace B-27 supplement.   Product Components Catalase T3 Putrescine Linoleic Acid DL-a-Tocopherol (Vitamin E) Oleic acid Lpoic acid Retinol acetate Glutathione I-carnitine Putrescine Linolenic Acid DL-a-Tocopherol Acetate Biotin Superoxide Dismutase Insulin D(+) Galactose Corticoseterone Progesterone Pipecolic acid Bovine Transferrin(HOLO) Ethanolamine Storage The products are shipped with ice pack and can be stored at -25~-15℃ for 2 year.  Cautions 1. Please operate with lab coats and disposable gloves,for your safety.        2. This product is for research use only.  

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Product description The Annexin V-FITC/PI Apoptosis Detection Kit uses FITC-labeled Annexin V as a probe to detect the onset of early apoptosis. The detection principle is as follows: in normal living cells, phosphatidylserine (PS) is located on the inner side of the cell membrane, but in early apoptotic cells, PS flips from the inner side of the cell membrane to the surface of the cell membrane and is exposed to the extracellular environment. Annexin V, a calcium-dependent phospholipid-binding protein with a molecular weight of 35-36 kDa, has high affinity for PS and can bind to the membrane of early apoptotic cells through the exposed PS on the outer side of the cell membrane.In addition, Propidium Iodide (PI) is provided in this kit to distinguish surviving early cells from necrotic or late apoptotic cells.PI is a nucleic acid dye that does not penetrate through the intact cell membranes of normal or early apoptotic cells, but can penetrate through the cell membranes of late apoptotic and necrotic cells and stain the nuclei red. Therefore, when Annexin V was used in combination with PI, PI was excluded from living cells (Annexin V-/PI-) and early apoptotic cells (Annexin V+/PI-), while late apoptotic and necrotic cells stained double-positively (Annexin V+/PI+) with both FITC and PI. This kit can be used for detection with flow cytometry or fluorescence microscopy. Specifications Components No. C331406E C331406S C331406M Size 20 T 50 T 100 T Shipping and Storage Transportation of ice packs: Store at -20°C in the dark to avoid repeated freezing and thawing, effective for one year. [Note]If multiple reuses are required within a short period, store in the dark at 4°C, effective for six months. Documents: Manuals C331406-EN-Manual.pdf

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Arcegel Matrix for Organoid culture, Phenol Red-Free, LDEV-Free is a soluble basement membrane preparation extracted from EHS mouse tumors rich in extracellular matrix proteins. Its main components are laminin, type IV collagen, heparan sulfate proteoglycan (HSPG), nestin as well as growth factors such as TGF-beta, EGF, IGF, FGF, tissue plasminogen activator and other growth factors contained in EHS tumors. At room temperature, it aggregates to form a biologically active three-dimensional matrix, which simulates the structure, composition, physical properties and functions of the cell basement membrane in vivo, which is beneficial to the culture and differentiation of cells in vitro. It can be used for studies of cell morphology, biochemical function, migration, invasion and gene expression. Arcegel Matrix is a sterile product, phenol red-free, LDEV-free, with a concentration of 8~12 mg/mL, and has been validated for organoid culture, meeting the requirements for organoid construction. Feature High Safety: No LDEV (lactate dehydrogenase elevating virus) Multiple Concentration: the concentration range from 8 to 20 mg/ml Batch Consistency: Strict process through the whole product life cycle including the production, quality, warehouse etc. Low Endotoxin: Endotoxin content <8 EU/ml Contamination Control: Adhere to the rigorous residue quality standards of mycoplasmas, bacteria, and fungi High yield:＞30L per single batch Wide Compatibility: Compatible with almost all types of cell culture medium Application 2D Culture Support Migration/Invasion Biochemical Research 3D & Organoid Culture Angiogenesis In Vivo Tumor Formation Specification Concentration 8~12 mg/mL Product Type Basement Membrane Matrix Endotoxin Level Low Transportation Conditions Dry Ice Transportation Product Line Arcegel  Form Frozen Phenol Red Indicator Contain Product specifications 5/10 mL Species EHS Mouse Tumors Classification Organoid Culture Serum Level None LDEV Detection None Components Components No. Name C231009E C231009S C231009 Arcegel Matrix for Organoid culture, Phenol Red-Free, LDEV-Free 5 mL 10 mL Shipping and Storage Transported on dry ice. Stored at -20°C with a shelf life of 2 years. Figures Cell application validation Figure 1. The application validation of Arcegel Matrix were as shown. The representative image of HepG2 cell stained with crystal violet after invasion (Figure 1A). The image of 3D culture of HepG2 cell for 4 days (Figure 1B). The representative bright field and fluorescent images of HUVEC cell angiogenesis (Figure 1C)                                   Figure 2. Intestinal organoids formation Documents: Manuals C231009-EN-Manual.pdf

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Arcegel Matrix GFR, LDEV-Free Matrigel is a soluble basement membrane preparation extracted from EHS mouse tumors rich in extracellular matrix proteins. Its main components are laminin, type IV collagen, heparan sulfate proteoglycan (HSPG), nestin as well as growth factors such as TGF-beta, EGF, IGF, FGF, tissue plasminogen activator and other growth factors contained in EHS tumors. At room temperature, it aggregates to form a biologically active three-dimensional matrix, which simulates the structure, composition, physical properties and functions of the cell basement membrane in vivo, which is beneficial to the culture and differentiation of cells in vitro. It can be used for studies of cell morphology, biochemical function, migration, invasion and gene expression. Arcegel Matrix is a sterile product with a concentration of 8~12 mg/mL, which meets a variety of experimental requirements. Feature High Safety: No LDEV (lactate dehydrogenase elevating virus) Multiple Concentration: the concentration range from 8 to 20 mg/ml Batch Consistency: Strict process through the whole product life cycle including the production, quality, warehouse etc. Low Endotoxin: Endotoxin content <8 EU/ml Contamination Control: Adhere to the rigorous residue quality standards of mycoplasmas, bacteria, and fungi High yield:＞30L per single batch Wide Compatibility: Compatible with almost all types of cell culture medium Application 2D Culture Support Migration/Invasion Biochemical Research 3D & Organoid Culture Angiogenesis In Vivo Tumor Formation Specification Concentration 8-12 mg/mL Product Type Basement Membrane Matrix Endotoxin Level Low Transportation Conditions Dry Ice Transportation Product Line Arcegel  Form Frozen Phenol Red Indicator Contain Product specifications 5/10 mL Species EHS Mouse Tumors Classification Basic Type Serum Level None LDEV Detection None Components Components No. Name C231003E C231003S C231003 Arcegel Matrix GFR, LDEV-Free 5 mL 10 mL Shipping and Storage Transported on dry ice. Stored at -20°C with a shelf life of 2 years. Figures Cell application validation Figure 1. The application validation of Arcegel Matrix were as shown. The representative image of HepG2 cell stained with crystal violet after invasion (Figure 1A). The image of 3D culture of HepG2 cell for 4 days (Figure 1B). The representative bright field and fluorescent images of HUVEC cell angiogenesis (Figure 1C)                                   Figure 2. Intestinal organoids formation Documents: Manuals C231003-EN-Manual.pdf

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Arcegel Matrix GFR, Phenol Red-Free, LDEV-Free is a soluble basement membrane preparation extracted from EHS mouse tumors rich in extracellular matrix proteins. Its main components are laminin, type IV collagen, heparan sulfate proteoglycan (HSPG), nestin as well as growth factors such as TGF-beta, EGF, IGF, FGF, tissue plasminogen activator and other growth factors contained in EHS tumors. At room temperature, it aggregates to form a biologically active three-dimensional matrix, which simulates the structure, composition, physical properties and functions of the cell basement membrane in vivo, which is beneficial to the culture and differentiation of cells in vitro. It can be used for studies of cell morphology, biochemical function, migration, invasion and gene expression. Arcegel Matrix is a sterile product and free of phenol red with a concentration of 8~12 mg/mL, which meets a variety of experimental requirements. Feature High Safety: No LDEV (lactate dehydrogenase elevating virus) Multiple Concentration: the concentration range from 8 to 20 mg/ml Batch Consistency: Strict process through the whole product life cycle including the production, quality, warehouse etc. Low Endotoxin: Endotoxin content <8 EU/ml Contamination Control: Adhere to the rigorous residue quality standards of mycoplasmas, bacteria, and fungi High yield:＞30L per single batch Wide Compatibility: Compatible with almost all types of cell culture medium Application 2D Culture Support Migration/Invasion Biochemical Research 3D & Organoid Culture Angiogenesis In Vivo Tumor Formation Specification Concentration 8-12 mg/mL Product Type Basement Membrane Matrix Endotoxin Level Low Transportation Conditions Dry Ice Transportation Product Line Arcegel  Form Frozen Phenol Red Indicator None Product specifications 5/10 mL Species EHS Mouse Tumors Classification Basic Type Serum Level None LDEV Detection None Components Components No. Name C231004E C231004S C231004 Arcegel Matrix GFR, Phenol Red-Free, LDEV-Free 5 mL 10 mL Shipping and Storage Stored at -20°C with a shelf life of 2 years. Transported on dry ice. Figures Cell application validation Figure 1. The application validation of Arcegel Matrix were as shown. The representative image of HepG2 cell stained with crystal violet after invasion (Figure 1A). The image of 3D culture of HepG2 cell for 4 days (Figure 1B). The representative bright field and fluorescent images of HUVEC cell angiogenesis (Figure 1C)                                   Figure 2. Intestinal organoids formation Documents: Manuals C231004-EN-Manual.pdf

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Arcegel Matrix hESC-Qualified, LDEV-Free is a soluble basement membrane preparation extracted from EHS mouse tumors rich in extracellular matrix proteins. Its main components are laminin, type IV collagen, heparan sulfate proteoglycan (HSPG), nestin as well as growth factors such as TGF-beta, EGF, IGF, FGF, tissue plasminogen activator and other growth factors contained in EHS tumors. At room temperature, it aggregates to form a biologically active three-dimensional matrix, which simulates the structure, composition, physical properties and functions of the cell basement membrane in vivo, which is beneficial to the culture and differentiation of cells in vitro. It can be used for studies of cell morphology, biochemical function, migration, invasion and gene expression. Arcegel Matrix is a sterile product, with a concentration of 8~12 mg/mL, validated for hESC cell culture to meet the requirements of stem cell growth. Feature High Safety: No LDEV (lactate dehydrogenase elevating virus) Multiple Concentration: the concentration range from 8 to 20 mg/ml Batch Consistency: Strict process through the whole product life cycle including the production, quality, warehouse etc. Low Endotoxin: Endotoxin content <8 EU/ml Contamination Control: Adhere to the rigorous residue quality standards of mycoplasmas, bacteria, and fungi High yield:＞30L per single batch Wide Compatibility: Compatible with almost all types of cell culture medium Application 2D Culture Support Migration/Invasion Biochemical Research 3D & Organoid Culture Angiogenesis In Vivo Tumor Formation Specification Concentration 8~12 mg/mL Product Type Basement Membrane Matrix Endotoxin Level Low Transportation Conditions Dry Ice Transportation Product Line Arcegel  Form Frozen Phenol Red Indicator Contain Product specifications 5/10 mL Species EHS Mouse Tumors Classification hESC Culture Serum Level None LDEV Detection None Components Components No. Name C231008E C231008S C231008 Arcegel Matrix hESC-Qualified, LDEV-Free 5 mL 10 mL Shipping and Storage Transported on dry ice. Stored at -20°C with a shelf life of 2 years. Figures Cell application validation Figure 1. The application validation of Arcegel Matrix were as shown. The representative image of HepG2 cell stained with crystal violet after invasion (Figure 1A). The image of 3D culture of HepG2 cell for 4 days (Figure 1B). The representative bright field and fluorescent images of HUVEC cell angiogenesis (Figure 1C)                                   Figure 2. Intestinal organoids formation Documents: Manuals C231008-EN-Manual.pdf

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Arcegel Matrix High Concentration, GFR, LDEV-Free is a soluble basement membrane preparation extracted from EHS mouse tumors rich in extracellular matrix proteins. Its main components are laminin, type IV collagen, heparan sulfate proteoglycan (HSPG), nestin as well as growth factors such as TGF-beta, EGF, IGF, FGF, tissue plasminogen activator and other growth factors contained in EHS tumors. At room temperature, it aggregates to form a biologically active three-dimensional matrix, which simulates the structure, composition, physical properties and functions of the cell basement membrane in vivo, which is beneficial to the culture and differentiation of cells in vitro. It can be used for studies of cell morphology, biochemical function, migration, invasion and gene expression. Arcegel Matrix is a sterile product with a high-concentration of more than 18 mg/mL, which meets a variety of experimental requirements. Feature High Safety: No LDEV (lactate dehydrogenase elevating virus) Multiple Concentration: the concentration range from 8 to 20 mg/ml Batch Consistency: Strict process through the whole product life cycle including the production, quality, warehouse etc. Low Endotoxin: Endotoxin content <8 EU/ml Contamination Control: Adhere to the rigorous residue quality standards of mycoplasmas, bacteria, and fungi High yield:＞30L per single batch Wide Compatibility: Compatible with almost all types of cell culture medium Application 2D Culture Support Migration/Invasion Biochemical Research 3D & Organoid Culture Angiogenesis In Vivo Tumor Formation Specification Concentration ≥18 mg/mL Product Type Basement Membrane Matrix Endotoxin Level Low Transportation Conditions Dry Ice Transportation Product Line Arcegel  Form Frozen Phenol Red Indicator Contain Product specifications 5/10 mL Species EHS Mouse Tumors Classification High Concentration Serum Level None LDEV Detection None Components Components No. Name C231007E C231007S C231007 Arcegel Matrix High Concentration, GFR, LDEV-Free 5 mL 10 mL Shipping and Storage Transported on dry ice. Stored at -20°C with a shelf life of 2 years. Figures Cell application validation Figure 1. The application validation of Arcegel Matrix were as shown. The representative image of HepG2 cell stained with crystal violet after invasion (Figure 1A). The image of 3D culture of HepG2 cell for 4 days (Figure 1B). The representative bright field and fluorescent images of HUVEC cell angiogenesis (Figure 1C)                                   Figure 2. Intestinal organoids formation Documents: Manuals C231007-EN-Manual.pdf

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Arcegel Matrix High Concentration, LDEV-Free is a soluble basement membrane preparation extracted from EHS mouse tumors rich in extracellular matrix proteins. Its main components are laminin, type IV collagen, heparan sulfate proteoglycan (HSPG), nestin as well as growth factors such as TGF-beta, EGF, IGF, FGF, tissue plasminogen activator and other growth factors contained in EHS tumors. At room temperature, it aggregates to form a biologically active three-dimensional matrix, which simulates the structure, composition, physical properties and functions of the cell basement membrane in vivo, which is beneficial to the culture and differentiation of cells in vitro. It can be used for studies of cell morphology, biochemical function, migration, invasion and gene expression. Arcegel Matrix is a sterile product with a concentration of more than 18 mg/mL, which meets a variety of experimental requirements, including in vivo tumorigenesis, angiogenesis research, tumor cell migration, 3D cell studies, and other applications. Feature High Safety: No LDEV (lactate dehydrogenase elevating virus) Multiple Concentration: the concentration range from 8 to 20 mg/ml Batch Consistency: Strict process through the whole product life cycle including the production, quality, warehouse etc. Low Endotoxin: Endotoxin content <8 EU/ml Contamination Control: Adhere to the rigorous residue quality standards of mycoplasmas, bacteria, and fungi High yield:＞30L per single batch Wide Compatibility: Compatible with almost all types of cell culture medium Application 2D Culture Support Migration/Invasion Biochemical Research 3D & Organoid Culture Angiogenesis In Vivo Tumor Formation Specification Concentration ≥18 mg/mL Product Type Basement Membrane Matrix Endotoxin Level Low Transportation Conditions Dry Ice Transportation Product Line Arcegel  Form Frozen Phenol Red Indicator Contain Product specifications 5/10 mL Species EHS Mouse Tumors Classification High Concentration Serum Level None LDEV Detection None Components Components No. Name C231005E C231005S C231005 Arcegel Matrix High Concentration, LDEV-Free 5 mL 10 mL Shipping and Storage Transported on dry ice. Stored at -20°C with a shelf life of 2 years. Figures Cell application validation Figure 1. The application validation of Arcegel Matrix were as shown. The representative image of HepG2 cell stained with crystal violet after invasion (Figure 1A). The image of 3D culture of HepG2 cell for 4 days (Figure 1B). The representative bright field and fluorescent images of HUVEC cell angiogenesis (Figure 1C)                                   Figure 2. Intestinal organoids formation Documents: Manuals C231005-EN-Manual.pdf

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Arcegel Matrix High Concentration, Phenol Red-Free, LDEV-Free is a soluble basement membrane preparation extracted from EHS mouse tumors rich in extracellular matrix proteins. Its main components are laminin, type IV collagen, heparan sulfate proteoglycan (HSPG), nestin as well as growth factors such as TGF-beta, EGF, IGF, FGF, tissue plasminogen activator and other growth factors contained in EHS tumors. At room temperature, it aggregates to form a biologically active three-dimensional matrix, which simulates the structure, composition, physical properties and functions of the cell basement membrane in vivo, which is beneficial to the culture and differentiation of cells in vitro. It can be used for studies of cell morphology, biochemical function, migration, invasion and gene expression. Arcegel Matrix is a sterile product with a concentration of more than 18 mg/mL, which meets a variety of experimental requirements, including in vivo tumorigenesis, angiogenesis studies, tumor cell migration, 3D cell studies and other applications. Feature High Safety: No LDEV (lactate dehydrogenase elevating virus) Multiple Concentration: the concentration range from 8 to 20 mg/ml Batch Consistency: Strict process through the whole product life cycle including the production, quality, warehouse etc. Low Endotoxin: Endotoxin content <8 EU/ml Contamination Control: Adhere to the rigorous residue quality standards of mycoplasmas, bacteria, and fungi High yield:＞30L per single batch Wide Compatibility: Compatible with almost all types of cell culture medium Application 2D Culture Support Migration/Invasion Biochemical Research 3D & Organoid Culture Angiogenesis In Vivo Tumor Formation Specification Concentration ≥18 mg/mL Product Type Basement Membrane Matrix Endotoxin Level Low Transportation Conditions Dry Ice Transportation Product Line Arcegel  Form Frozen Phenol Red Indicator None Product specifications 5/10 mL Species EHS Mouse Tumors Classification High Concentration Serum Level None LDEV Detection None Components Components No. Name C231006E C231006S C231006 Arcegel Matrix High Concentration, Phenol Red-Free, LDEV-Free 5 mL 10 mL Shipping and Storage Transported on dry ice. Stored at -20°C with a shelf life of 2 years. Figures Cell application validation Figure 1. The application validation of Arcegel Matrix were as shown. The representative image of HepG2 cell stained with crystal violet after invasion (Figure 1A). The image of 3D culture of HepG2 cell for 4 days (Figure 1B). The representative bright field and fluorescent images of HUVEC cell angiogenesis (Figure 1C)                                   Figure 2. Intestinal organoids formation Documents: Manuals C231006-EN-Manual.pdf

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Arcegel Matrix LDEV-Free is a soluble basement membrane preparation extracted from EHS mouse tumors rich in extracellular matrix proteins. Its main components consist of laminin, type IV collagen, heparan sulfate proteoglycan (HSPG), nestin as well as growth factors such as TGF-beta, EGF, IGF, FGF, tissue plasminogen activator and other growth factors contained in EHS tumors. At room temperature, it aggregates to form a biologically active three-dimensional matrix, which simulates the structure, composition, physical properties and functions of the cell basement membrane in vivo, which is beneficial to the culture and differentiation of cells in vitro. It can be used for studies of cell morphology, biochemical function, migration, invasion and gene expression. Arcegel Matrix is a sterile product with a concentration of 8~12 mg/mL, which meets a variety of experimental requirements, including angiogenesis studies and tumor cell migration. Feature High Safety: No LDEV (lactate dehydrogenase elevating virus) Multiple Concentration: the concentration range from 8 to 20 mg/ml Batch Consistency: Strict process through the whole product life cycle including the production, quality, warehouse etc. Low Endotoxin: Endotoxin content <8 EU/ml Contamination Control: Adhere to the rigorous residue quality standards of mycoplasmas, bacteria, and fungi High yield:＞30L per single batch Wide Compatibility: Compatible with almost all types of cell culture medium Application 2D Culture Support Migration/Invasion Biochemical Research 3D & Organoid Culture Angiogenesis In Vivo Tumor Formation Specification Concentration 8-12 mg/mL Product Type Basement Membrane Matrix Endotoxin Level Low Transportation Conditions Dry Ice Transportation Product Line Arcegel  Form Frozen Phenol Red Indicator Contain Product specifications 5/10 mL Species EHS Mouse Tumors Classification Basic Type Serum Level None LDEV Detection None Components Components No. Name C231001E C231001S C231001 Arcegel Matrix LDEV-Free 5 mL 10 mL Shipping and Storage Transported on dry ice. Stored at -20°C with a shelf life of 2 years. Figures Cell application validation Figure 1. The application validation of Arcegel Matrix were as shown. The representative image of HepG2 cell stained with crystal violet after invasion (Figure 1A). The image of 3D culture of HepG2 cell for 4 days (Figure 1B). The representative bright field and fluorescent images of HUVEC cell angiogenesis (Figure 1C)                                   Figure 2. Intestinal organoids formation Documents: Manuals C231001-EN-Manual.pdf

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Arcegel Matrix Phenol Red-Free, LDEV-Free is a soluble basement membrane preparation extracted from EHS mouse tumors rich in extracellular matrix proteins. Its main components are laminin, type IV collagen, heparan sulfate proteoglycan (HSPG), nestin as well as growth factors such as TGF-beta, EGF, IGF, FGF, tissue plasminogen activator and other growth factors contained in EHS tumors. At room temperature, it aggregates to form a biologically active three-dimensional matrix, which simulates the structure, composition, physical properties and functions of the cell basement membrane in vivo, which is beneficial to the culture and differentiation of cells in vitro. It can be used for studies of cell morphology, biochemical function, migration, invasion and gene expression. Arcegel Matrix is a sterile product, free of phenol red, with a concentration of 8~12 mg/mL, which meets a variety of experimental requirements, including experiments requiring color detection such as fluorescence assays. Feature High Safety: No LDEV (lactate dehydrogenase elevating virus) Multiple Concentration: the concentration range from 8 to 20 mg/ml Batch Consistency: Strict process through the whole product life cycle including the production, quality, warehouse etc. Low Endotoxin: Endotoxin content <8 EU/ml Contamination Control: Adhere to the rigorous residue quality standards of mycoplasmas, bacteria, and fungi High yield:＞30L per single batch Wide Compatibility: Compatible with almost all types of cell culture medium Application 2D Culture Support Migration/Invasion Biochemical Research 3D & Organoid Culture Angiogenesis In Vivo Tumor Formation Specification Concentration 8-12 mg/mL Product Type Basement Membrane Matrix Endotoxin Level Low Transportation Conditions Dry Ice Transportation Product Line Arcegel  Form Frozen Phenol Red Indicator None Product specifications 5/10 mL Species EHS Mouse Tumors Classification Basic Type Serum Level None LDEV Detection None Components Components No. Name C231002E C231002S C231002 Arcegel Matrix Phenol Red-Free, LDEV-Free 5 mL 10 mL Shipping and Storage Stored at -20°C with a shelf life of 2 years. Transported on dry ice. Figures Cell application validation Figure 1. The application validation of Arcegel Matrix were as shown. The representative image of HepG2 cell stained with crystal violet after invasion (Figure 1A). The image of 3D culture of HepG2 cell for 4 days (Figure 1B). The representative bright field and fluorescent images of HUVEC cell angiogenesis (Figure 1C)                                   Figure 2. Intestinal organoids formation Documents: Manuals C231002-EN-Manual.pdf

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Product description Caerulein is a decapeptide and an agonist of cholecystokinin (CCK) receptor. As a cholecystokinin analog, it can stimulate gastric, bile duct and pancreatic secretion, acting on pancreatic alveolar cells, leading to the secretion of large amounts of digestive enzymes and pancreatic juice, leading to acute edematous pancreatitis. Caerulein can be used to study signal transduction pathways mediated by NF-κB upregulated proteins such as intercellular adhesion molecule (ICAM-1), inflammation-related factors such as NADPH oxidase and Janus kinase. Cerulein can also be used to test gallbladder function and prevent gallbladder pain, renal colic and intermittent claudication pain. It is generally considered an antagonist of endogenous orphins and can be used in conjunction with LPS to establish animal models of pancreatitis. Cerulein has been successfully used to establish acute pancreatitis (AP) models in rats, mice, dogs, Syrian hamsters and other animals. The modeling mechanism is as follows: 1.Upregulate the expression of intercellular adhesion molecule (ICAM-1) in pancreatic acinar cells by stimulating intracellular NF-KB. Surface ICAM-1 in turn promotes neutrophil adhesion to acinar cells thereby enhancing pancreatic inflammatory effects; 2.Induction of pancreatic inflammation by disrupting digestive enzyme secretion and causing cytoplasmic vacuolization and acinar cell death, leading to pancreatic edema; 3.Activate inflammation-promoting factors. Specifications English Synonym Caerulein Sulfated; Cerulein, Ceruletide; [Tyr(SO3H)4]Caerulein CAS NO. 17650-98-5 Formula C58H73N13O21S2 Molecular Weight 1352.4 Appearance White powder Purity ≥97% Solubility Soluble in DMSO, insoluble in water Sequence pGlu-Gln-Asp-Tyr(SO3H)-Thr-Gly-Trp-Met-Asp-Phe-NH2 Components Components No. C331603E Size 1 mg Storage Dry ice shipping. Powder: -85℃~-65℃, valid for 2 years; -15℃~-25℃, valid for 1 year.Solution should be stored at -85°C to -65°C with a shelf life of 6 months; or at -15°C to -25°C with a shelf life of 1 month. Keep refrigerated and dry, avoiding repeated freeze-thaw cycles. Documents: Manuals C331603-EN-Manual.pdf

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Product description Cell Counting Kit-8, abbreviated as CCK-8, is a rapid and highly sensitive assay for cell proliferation and cytotoxicity based on WST-8 (chemical name: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfophenyl)-2H-tetrazolium monosodium salt), which is an upgraded version of MTT. WST-8 is an upgraded product of MTT, the working principle is: in the presence of electronically coupled reagents, it can be reduced by the dehydrogenase enzyme in the mitochondria to generate a highly water-soluble orange-yellow dirty product (formazan). The shade of color is directly proportional to cell proliferation and inversely proportional to cytotoxicity. The OD value is measured at 450 nm using an enzyme marker, indirectly reflecting the number of viable cells.CCK-8 method has a wide range of applications, such as drug screening, cell proliferation assay, cytotoxicity assay, tumor sensitivity assay, and the assessment of biological factor activity. Components Components No. C331301E C331301S C331301M C331301L C331301T Size 100 T 500 T 1000 T 3×1000 T 10×1000 T Shipping and Storage Ice packs shipping. 2~8℃ dry and lightproof storage, valid for one year.-20℃ dry and lightproof storage, valid for two years. Documents: Manuals C331301-EN-Manual.pdf

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Product description The 3DCultr Cell recovery solution for Organoid is designed for the detection, observation, and passaging of organoids cultured on extracellular matrix (ECM). It contains specific components that enable the separation of organoids from the matrix gel and digestion into small cell clusters or single-cell states. The entire process is gentle and rapid, while maintaining cell viability. The product does not contain any biological enzymes. Specifications Catalog Number C231131E/C231131S Specifications 30 mL/100 mL Components Component Number Component Name Storage Temperature C231131E C231131S C231131 3DCultr Cell recovery solution for Organoid 2~8℃ 30 mL 100 mL Storage Shipment with ice packs. Store at 2~8°C. Shelf life: 1 year.

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Product description Celthy Ultra Liposomal Transfection Reagent is a versatile and highly efficient liposomal transfection reagent developed based on the latest nanotechnology. It is suitable for transfecting DNA, RNA, and oligonucleotides. This reagent exerts a gentle cellular effect with low toxicity and demonstrates high transfection efficiency even in difficult-to-transfect cells. Moreover, it is not influenced by serum and can completely replace Lipo3000 transfection reagent. Specifications Product Number C130003S/C130003M Product Specifications 0.5 mL/1 mL Components Component Number Component Name C130003S C130003M C130003-A Solution A 0.5 mL 1 mL C130003-B Solution B 0.5 mL 1 mL Storage Store at 2~8°C, with a shelf life of 1 year. Do not freeze! Documents: Manuals C130003-EN-Manual.pdf  

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Product description Celthy Univer Liposomal Transfection Reagent is a versatile liposome transfection reagent, suitable for DNA, RNA and oligonucleotide transfection, with high transfection efficiency for most eukaryotic cells. Its unique formulation allows for direct addition to the culture medium without serum affecting transfection efficiency, thus reducing serum-related cell damage. There is no need to remove the nucleic acid-Celthy complex or replace with fresh medium after transfection, and it can also be removed after 4~6 hours. Celthy is supplied in sterile liquid form. Usually, for 24-well plate transfection, about 1.5 μL each time, 1 mL of Celthy can do about 660 transfections; for 6-well plate, about 6 μL each time, 1 mL of Celthy can do about 660 transfections. Specifications Catalog Number C130002S/C130002M Specifications 0.5 mL/1 mL Properties Form Liquid Serum Compatible Yes Cell Type Established Cell Lines Sample Type Plasmid DNA, Synthetic siRNA Transfection Technique Lipid-Based Transfection Storage Store at 2~8°C, with a shelf life of 1 year. Do not freeze! Documents: Manuals C130002-EN-Manual.pdf  

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Product description D-Luciferin is a commonly used substrate for luciferase in the field of biotechnology, particularly in in vivo live imaging techniques. When fluorescein is in excess, the quantum number of light produced is equal to Luciferase concentrations were positively correlated (see figure below). Plasmids carrying luciferase encoding gene (Luc) were transfected into cells and introduced into study animals such as rats and mice. Subsequently, D-luciferin is injected, and changes in light intensity are detected using bioluminescence imaging (BLI) to monitor disease progression or therapeutic efficacy of drugs in real-time. Alternatively, the impact of ATP on this reaction system can be utilized to indicate changes in energy or vital signs based on variations in bioluminescence intensity. D-Luciferin is also commonly used in in vitro research, including luciferase and ATP level analysis; reporter gene analysis; high-throughput sequencing; and various contamination detections. Currently, there are three forms of the product: D-luciferin (free acid), D-luciferin salt (sodium salt and potassium salt). The main difference lies in their dissolution properties: the former has weaker water solubility and solubility in buffer systems, unless dissolved in weak bases such as low concentration NaOH and KOH solutions. It can be dissolved in methanol and DMSO; the latter can be easily dissolved in water or buffer solutions, making it convenient to use, with non-toxic solvents, especially suitable for in vivo experiments. After being prepared into solutions, these three forms of the product have no substantial differences in most applications. Specifications English synonym (S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid potassium salt; D-Luciferin firefly, potassium salt CAS NO. 115144-35-9 Formula C11H7N2O3S2K Molecular weight 318.42 g/mol Appearance Light yellow powder Solubility Soluble in water(60 mg/mL) Components Components No. C331501E C331501S C331501M C331501L Size 100 mg 500 mg 1 g 5 g Storage Store at -20°C in a dry and dark place. Shelf life is 1 year. Documents: Manuals C331501-EN-Manual.pdf

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Product description D-luciferin is a common substrate for Luciferase and is widely used throughout biotechnology, especially in vivo imaging technology. Its mechanism of action involves oxidation and luminescence of luciferin (substrate) in the presence of ATP and luciferase enzyme. When luciferin is present in excess, the number of light photons produced is directly proportional to the concentration of luciferase enzyme (as illustrated in the diagram below). Plasmids carrying luciferase encoding gene (Luc) were transfected into cells and introduced into study animals such as rats and mice In vivo, Subsequent injection of luciferin allows for real-time monitoring of changes in light intensity using bioluminescence imaging (BLI), enabling the real-time monitoring of disease progression or the therapeutic efficacy of drugs. Additionally, the impact of ATP on this reaction system can be utilized, with changes in bioluminescence intensity indicating energy levels or vital signs. D-luciferin is also commonly used in vitro for various research purposes, including analysis of luciferase activity and ATP levels, reporter gene assays, high-throughput sequencing, and various contamination detection assays.D-luciferin (free acid) has weak solubility in water and in buffered solutions unless dissolved in weak bases such as NaOH and KOH solutions. It is soluble in methanol (10 mg/mL) and DMSO (50 mg/mL). However, D-luciferin sodium salt and potassium salt forms can be easily and rapidly dissolved in water or buffered solutions, making them convenient to use. These solvents are non-toxic and particularly suitable for in vivo experiments. In most applications, there are no substantial differences among these three forms of D-luciferin once they are dissolved in solution. Specifications English synonym (S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid sodium salt; D-Luciferin firefly, sodium salt monohydrate; CAS NO. 103404-75-7 Formula NaC11H7N2O3S2·H2O Molecular weight 320.32 g/mol Appearance Light yellow powder Solubility Solube in water(100 mg/mL) Purity (HPLC) ≥95% Components Components No. C331502E C331502S C331502M C331502L C331502T Size 100 mg 500 mg 1 g 5 g 10 g Storage Dry ice shipping. -20℃ storage, valid for one year. Documents: Manuals C331502-EN-Manual.pdf

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Product description Dextran sulfate sodium(DSS) is a polyanionic derivative of dextran, formed by the esterification reaction of dextran and chlorosulfonic acid. It is commonly used in colitis modeling research. DSS has several characteristics:1) It is a polyanionic compound soluble in water, forming a colorless aqueous solution; 2) It exhibits high purity and good stability; 3) It is naturally degradable. Inflammatory bowel disease (IBD) is a chronic, relapsing gastrointestinal infection that increases the risk of intestinal tumors, mainly including ulcerative colitis (UC) and Crohn’s disease (CD). Since its first reported use in 1985 for inducing ulcerative colitis-like symptoms in mice, using dextran sulfate sodium (DSS), numerous studies have confirmed the similarity of the DSS colitis model to human ulcerative colitis.The histological features, clinical manifestations, the disease site and cytokine proliferation of DSS colitis model are very similar to human ulcerative colitis (UC). The modeling conditions and operation methods of this model are simple, cost-effective, reproducible, easy to master, and can be easily adapted and standardized. Depending on the experimental objectives, the DSS concentration and administration timing can be adjusted to establish acute, chronic, or alternate acute-chronic models. Specifications English Synonym Dextran Sulfate Sodium Salt, DSS; Dextran Sodium sulfate CAS NO. 9011-18-1 Formula (C6H7Na3O14S3) n Molecular Weight 36, 000 ~50, 000 Da Appearance White or off-white powder Solubility Soluble in water, slightly soluble in ethanol. Components Components No. C331601E C331601S C331601M C331601L Size 25 g 100 g 500 g 1 kg Storage The product is shipped and stored at room temperature, valid for 2 years. Documents: Manuals C331601-EN-Manual.pdf

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Product description Regular monitoring during cell culture is effective in preventing mycoplasma contamination. However, if cells are already contaminated with mycoplasma, prompt treatment is necessary. The optimal treatment approach involves high-pressure sterilization of contaminated cells followed by disposal to prevent contamination of other clean cell lines. If contaminated cells are valuable, mycoplasma contamination must be removed. Since mycoplasma lacks a cell wall, traditional antibiotics are generally ineffective against them. This product is an improved mixture specifically designed for mycoplasma removal. It achieves excellent mycoplasma clearance by inhibiting the synthesis of essential proteins required for DNA and mycoplasma growth. It is non-toxic to cells, thus maximizing the preservation of your valuable cells and minimizing losses caused by mycoplasma contamination. Specifications Catalog Number C230102S/C230102M Specifications 1 mL/5×1 mL Storage Transportation with Ice Packs. Store in a dark place at -20°C. Shelf life is 18 months. If not in use for an extended period, store in a dark place. Notes 1. Before using this reagent, please read the instruction manual carefully. 2. Standardized operations, including the preparation of the reaction system, sample handling, and sample addition, should be followed throughout the experiment. 3. For your safety and health, wear laboratory coats and disposable gloves during operation. 4. For research use only. Instructions 1. Before use, ensure the bottle cap is tightly sealed, thaw the solution to room temperature, gently vortex to mix thoroughly, and wipe the surface of the bottle with 70% ethanol before placing it in a laminar flow hood. 2. Follow the instructions below when using DfCell reagent to treat cells 3. After splitting contaminated cells, add 10 µL of DfCell reagent solution to 10 mL of culture medium and culture for 7 days. During this period, when changing the medium, add DfCell reagent in the same proportion. 4. After 7 days of cell culture, switch to a medium without mycoplasma elimination reagent and culture for 2 days. Take 10 µL of cell culture supernatant for mycoplasma detection to assess the clearance effect. 【Note】 a. Due to variations in cell tolerance to this product (in most cases, low cytotoxicity), if the product exhibits toxicity to cells or slows cell growth during use, it can be diluted at a ratio of 1:2000 before use. b. If mycoplasma contamination is severe and 7 days of treatment with the elimination reagent does not completely clear the mycoplasma, the treatment time can be extended appropriately. c. After treatment, it is recommended to use the DfCell Mycoplasma LAMP Detection Kit(Cat#C230105) or DfCell Mycoplasma RT-qPCR Detection Kit (Cat#C230106) to assess the removal effect. Documents: Manuals C230102-EN-Manual.pdf

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Product description The DfCell  Mycoplasma LAMP Detection Kit is a rapid detection product developed using Arcegen's unique isothermal amplification technology for detecting mycoplasma contamination in cell culture fluids. The main principle is that if cell cultures are contaminated with mycoplasma, the conserved sequence of mycoplasma DNA will be amplified rapidly and abundantly, causing the reaction mixture to change from blue-purple to sky blue, which can be visually distinguished without the need for electrophoresis. The DfCell Mycoplasma LAMP Detection Kit can detect multiple types of mycoplasma, including the 8 common mycoplasma strains commonly found in cell culture. Traditional nested PCR mycoplasma detection methods are prone to false negative results due to the presence of inhibitors in the cell culture supernatant and require opening the lid for electrophoresis after the reaction, increasing the risk of false positive results due to contamination. The DfCell LAMP Kit completely eliminates these drawbacks and has high sensitivity and accuracy.  Specifications Catalog Number C230105S/C230105M Specifications 25 T/100 T Components Component Number Component Name C230105S C230105M C230105-A LAMP Mix 600 μL 600 μL×4 C230105-B LAMP Primer 25 μL 25 μL×4 C230105-C Positive Control 10 μL 10 μL×4 C230105-D Mineral Oil 500 μL 500 μL×4 Storage Transportation with Ice Packs. Store in a dark place at -20°C. Shelf life is 18 months. If not in use for an extended period, please store in a dark place. Documents: Manuals C230105-EN-Manual.pdf  

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Product description DfCell Mycoplasma Off Spray effectively inhibits the growth and contamination of fungi (including spores), bacteria (including spores), mycoplasma, and viruses (including HIV and Hepatitis B). Its active ingredient is a quaternary ammonium derivative. It is free from mercury, formaldehyde, phenol, alcohol, and other irritants, non-toxic, and biodegradable. It does not cause any damage to general work surfaces and equipment. This product is a ready-to-use solution, to be used directly. Specifications Catalog Number C230101E/C230101S/C230101M Specifications 500 mL /2×500 mL/10×500 mL Storage Store and transport at room temperature. Shelf life is 18 months. Notes 1. Please read the entire instruction manual carefully before using this reagent. 2. Standardized operations, including the preparation of the reaction system, sample handling, and sample addition, should be followed throughout the experiment. 3. For your safety and health, wear laboratory coats and disposable gloves during operation. 4. For research use only. Instructions 1. Spray the CO2 incubator once every two weeks. There is no need to empty the incubator before spraying. 2. Spray the laminar flow hood before each use and allow it to air dry naturally. Documents: Manuals C230101-EN-Manual.pdf

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Product description DfCell Mycoplasma qPCR Detection Kit is a rapid qualitative test based on Nucleic Acid Amplification Techniques (NAT) designed to detect potential Mycoplasma contamination in raw materials, cell banks, virus seeds, virus or cell harvests, and therapeutic cell products. This assay kit utilizes quantitative PCR technology employing a multiplex PCR approach with two fluorescent probes, FAM and VIC, to respectively detect the target sequence and internal reference. It covers over 100 Mycoplasma DNA sequences and has undergone rigorous validation for specificity, detection limit, and durability according to EP 2.6.7 standards. The detection limit meets the requirement of ≤10 CFU/mL, demonstrating high sensitivity, excellent specificity, and safety. Specifications Product Number C230106E / C230106S Specifications 25 T / 100 T Components Component Number Component Name C230106E C230106S C230106-A 4×qPCR Reaction Buffer 250 μL 1 mL C230106-B Primer & Probe Mix 25 μL 100 μL C230106-C Internal Control (IC) 25 μL 100 μL C230106-D* Positive Control (PC) 500 μL 2 mL C230106-E** DNA Dilution Buffer 1 mL 4×1 mL C230106-F*** ddH2O 500 μL 2×1 mL *PC：Positive control solution, with a concentration of 1, 000 copies/µL; **DNA Dilution Buffer: Used for sample and IC dilution, as well as template for NTC and NC; ***ddH2O: Used for preparing qPCR Mix system. Storage Store at temperatures ranging from -15°C to -25°C with a shelf life of one year. Please note that component C230106-B should be stored protected from light. Documents: Manuals C230106-EN-Manual.pdf

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Product description Fetal bovine serum is taken from cardiac blood sampling from fetal bovines born by caesarean section. After removing cells, fibrin, and clotting factors, serum contains a large number of nutrients and macromolecular factors that are essential for cell growth. Bovine serum albumin is the most important ingredient, along with growth factors and small molecules such as amino acids, sugars, lipids, and hormones, which are essential for the maintenance and growth of cultured cells. Arcegen's Fetal Bovine Serum Platinum is sourced from Uruguay, the only South American country recognized for exporting bovine serum to China. Fully automated canning after 3 rounds of 100 nm filtration, mycoplasma, virus screening, and rigorous inspection and testing at the place of origin. Standardized production, quality assurance, rich in nutrients required for cell growth, suitable for cultivating a variety of cell lines. Components Components No. C230211S Size 500 mL Storage Dry ice shipping. -20℃ storage, valid for five years. Documents: Manuals C230211-EN-Manual.pdf

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Human BMP-4 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of BMP-4 in serum, plasma, and cell culture supernatants. Specific anti-BMP-4 antibodies are pre-coated onto high-affinity enzyme plates. Standards and samples are added to the wells of the enzyme plate, and after incubation, BMP-4 present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for binding and incubated, followed by washing, and then the enzyme complex (Streptavidin-HRP) is added for binding and incubated. After washing, a colorimetric substrateTMB is added for color development, avoiding light. The intensity of the color reaction is proportional to the concentration of BMP-4 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm). Bone Morphogenetic Protein 4(BMP4) belongs to the TGF-β superfamily, playing essential roles in many developmental processes, including neurogenesis, vascular development, angiogenesis and osteogenesis. BMP-4 induces cartilage and bone formation. Also acts in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction. Specification Item Number P162015S / P162015E Specification 48 T / 96 T Detection Range 15.63-1000 pg/mL Detection Method Sandwich ELISA Detection Time 4.5 hours Sensitivity 11.2 pg/mL Dilution Linearity 86 - 127% Recovery Rate 75 - 126% Intra-assay Variability 4.0% Inter-assay Variability 5.0% Components Component Number Component Name Storage Temperature P162015S P162015E P162015-A Plate 2~8℃ 48 T 96 T P162015-B Standard 2~8℃ 1 tube 2 tubes P162015-C Detection Antibody 2~8℃ 60 μL 120 μL P162015-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL P162015-E Sample Dilution Buffer 2~8℃ 8 mL 15 mL P162015-F Antibody/Enzyme Dilution Buffer 2~8℃ 15 mL 30 mL P162015-G 20x Wash Buffer 2~8℃ 25 mL 50 mL P162015-H Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL P162015-I Stop Solution Room Temperature 5 mL 10 mL P162015-J Plate Sealers Room Temperature 3 pieces 5 pieces Storage The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year. Table 1 Reagent Storage Table After First Use  Material Name Storage Conditions Plate   Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption. Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination. Detection Antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination. Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination. Sample Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination. 20x Wash Buffer Store at 2~8°C for 1 month, avoiding contamination. Antibody/Enzyme Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination. Substrate solution Store at 2~8°C for 1 month, avoiding light exposure. Stop Solution Can be stored at room temperature. Plate Sealers Can be stored at room temperature.                                    Documents: Manuals P162015-EN-Manual.pdf

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Human Bone Morphogenetic Protein-7 (Human BMP-7) is a member of the TGF-β superfamily and is considered one of the most potent synthetic factors for chondrocytes. The growth factor domain of human BMP-7 shares 98% amino acid sequence identity with mouse and rat BMP-7. BMP-7 acts in various organ systems. It promotes new bone formation and the development of renal units, inhibits differentiation of prostate epithelium, and antagonizes epithelial-mesenchymal transition. Bone Morphogenetic Protein (BMP) signaling regulates a series of cellular processes and plays an important role in early embryonic patterning. It is known that BMP-7 expression has a protective effect on renal injury during diabetic nephropathy and disappears in the early stages of diabetic nephropathy progression. Potential clinical applications of BMP-7 include its neuroprotective effects in stroke animal models. The Arcegen Human BMP-7 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitatively measuring BMP-7 in serum, plasma, and cell culture supernatants. Specific anti-BMP-7 antibodies are precoated onto high-affinity enzyme plates. Standard samples and test samples are added to the enzyme plate wells and incubated. BMP-7 in the samples binds to the solid-phase antibodies. After washing away unbound substances, detection antibodies are added and incubated, followed by washing and addition of enzyme conjugate (Streptavidin-HRP) for incubation. After washing, TMB substrate solution is added for color development in the dark. The intensity of the color reaction is directly proportional to the concentration of BMP-7 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at a wavelength of 450 nm (with a reference wavelength of 570 - 630 nm). Specification Item Number P162014S / P162014E Specification 48 T / 96 T Detection Range 62.5-4000 pg/mL Detection Method Sandwich ELISA Detection Time 4.5 hours Sensitivity 44.6 pg/mL Dilution Linearity 86 - 127% Recovery Rate 81 - 126% Intra-assay Variability 3.3% Inter-assay Variability 4.2% Components Component Number Component Name Storage Temperature P162014S P162014E P162014-A ELISA Plate 2~8℃ 48 T 96 T P162014-B Standard 2~8℃ 1 tube 2 tubes P162014-C Detection Antibody 2~8℃ 60 μL 120 μL P162014-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL P162014-E Sample Dilution Buffer 2~8℃ 8 mL 15 mL P162014-F Antibody/Enzyme Dilution Buffer 2~8℃ 15 mL 30 mL P162014-G 20x Wash Buffer 2~8℃ 25 mL 50 mL P162014-H Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL P162014-I Stop Solution Room Temperature 5 mL 10 mL P162014-J Plate Sealant Film Room Temperature 3 pieces 5 pieces Storage The kit can be stored at 2~8℃ or according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working concentration reagents should be used immediately and discarded, and they should not be reused. The shelf life is 1 year. Table 1 Reagent Storage Table After First Use  Component Names Storage Conditions ELISA Plate   Unused strips can be returned to the aluminum foil bag,tightly sealed,and stored at 2-8°C to avoid moisture absorption. Standard Use within 48 hours after dissolution,store at 2-8°C to avoid contamination. Detection Antibody Use within 48 hours after dilution,store at 2~8°C to avoid contamination. Enzyme Conjugate Use within 48 hours after dilution,store at 2~8°C to avoid contamination. Sample Dilution Buffer Store at 2~8°C for 1 month,avoiding contamination. Antibody/Enzyme Dilution Buffer Store at 2~8°C for 1 month,avoiding contamination. 20x Wash Buffer Store at 2~8°C for 1 month,avoiding contamination. Substrate Solution Store at 2~8°C for 1 month,avoiding light exposure. Stop Solution Can be stored at room temperature. Plate Sealant Film Can be stored at room temperature.                                    Documents: Manuals P162014-EN-Manual.pdf

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Human CXCL10 ELISA Kit is an in vitro enzyme-linked immunosorbent assay kit used for the quantitative determination of Human CXCL10 (Interferon-γ-induced Protein 10) in serum, plasma, and cell culture supernatants. Specific antibodies against Human CXCL10 are pre-coated on high-affinity enzyme plates. Standard samples and test samples are added to the wells of the enzyme plate and, after incubation, the CXCL10 present in the samples binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody (biotin-labeled) is added and incubated to bind. Following another wash, an enzyme conjugate (HRP-labeled Streptavidin) is added and incubated to bind. After washing, aTMB chromogenic substrate is added for color development in the absence of light. The intensity of the color reaction is proportional to the concentration of CXCL10 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm (primary wavelength) and 630 nm (secondary wavelength). IP-10 was initially identified as an IFN-γ-induced gene in monocytes, fibroblasts, and endothelial cells. The mouse homolog of human IP-10, CRG-2, shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of IP-10 determines its membership in the CXC chemokine subfamily. Specification Item Number P162021S / P162021E Specification 48 T / 96 T Detection Range 15.63-1000 pg/mL Detection Method Sandwich ELISA Detection Species Human Detection Time 4.75 hours Sensitivity 4.460 pg/mL Dilution Linearity 72.2 - 119.4% Recovery Rate 73.3 - 129.6% Intra-assay Variability 9.2% Intra-assay Variability 6.5% Components Component Number Component Name Storage Temperature P162021S P162021E P162021-A ELISA Plate 2~8℃ 48 T 96 T P162021-B Standard 2~8℃ 1 tube 2 tubes P162021-C Detection Antibody 2~8℃ 1 tube 2 tubes P162021-D Enzyme Conjugate 2~8℃(Avoid Light) 150 μL 300 μL P162021-E 5x Dilution Buffer 2~8℃ 12 mL 25 mL P162021-F 20x Wash Buffer 2~8℃ 25 mL 50 mL P162021-G Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL P162021-H Stop Solution Room Temperature 5 mL 10 mL P162021-I Plate Sealers Room Temperature 3 pieces 5 pieces Storage The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year. Table 1 Reagent Storage Table After Initial Use  Material Name Storage Conditions Plate   Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption. Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination. Detection Antibody Use within 48 hours after dissolution, store at 2~8°C to avoid contamination. Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination. 5×Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination. 20×Wash Buffer Store at 2~8°C for 1 month, avoiding contamination. Substrate solution Store at 2~8°C for 1 month, avoiding light exposure. Stop Solution Can be stored at room temperature. Plate Sealers Can be stored at room temperature.                                    Documents: Manuals P162021-EN-Manual.pdf

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Human Fibroblast Growth Factor 10 (FGF-10), also known as Keratinocyte Growth Factor 2 (KGF-2), belongs to the FGF family and is a approximately 20 kDa protein with a region rich in serine near its N-terminus. It shares 93% and 96% amino acid sequence identity with mouse and rat FGF-10, respectively. FGF-10 is considered important during embryonic development and also plays a significant role in adipogenesis. FGF-10 is expressed in the pre-second heart field and, together with Fibroblast Growth Factor 8 (FGF-8), promotes the proliferation of cardiac progenitor cells forming the arterial pole of the heart. The Arcegen Human FGF-10 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of FGF-10 in serum, plasma, and cell culture supernatants. Specific anti-FGF-10 antibodies are pre-coated onto high-affinity enzyme plates. Standards and samples are added to the wells of the enzyme plate, and after incubation, FGF-10 present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for binding and incubated, followed by washing, and then the enzyme complex (Streptavidin-HRP) is added for binding and incubated. After washing, a colorimetric substrateTMB is added for color development, avoiding light. The intensity of the color reaction is proportional to the concentration of FGF-10 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570~630 nm). Specification Item Number P162002E / P162002S Specification 48 T / 96 T Detection Range 1~64 ng/mL Detection Method Sandwich ELISA Detection Time 4.5 hours Sensitivity 0.69 ng/mL Dilution Linearity 77~122% Recovery Rate 77~121% Intra-assay Variability 5.7% Inter-assay Variability 9.2% Components Component Number Component Name Storage Temperature P162002E P162002S P162002-A ELISA Plate 2~8℃ 48 T 96 T P162002-B Standard 2~8℃ 1 tube 2 tubes P162002-C Detection Antibody 2~8℃ 60 μL 120 μL P162002-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL P162002-E Sample Dilution Buffer 2~8℃ 8 mL 15 mL P162002-F Antibody/Enzyme Dilution Buffer 2~8℃ 15 mL 30 mL P162002-G 20x Wash Buffer 2~8℃ 25 mL 50 mL P162002-H Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL P162002-I Stop Solution Room Temperature 5 mL 10 mL P162002-J Plate Sealant Film Room Temperature 3 pieces 5 pieces Shipping and Storage The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year. Table 1 Reagent Storage Table After Initial Use  Material Name Storage Conditions ELISA plate  Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption. Standard sample Use within 48 hours after dissolution, store at 2~8°C to avoid contamination. Detecting antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination. Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination. Sample diluent Store at 2~8°C for 1 month, avoiding contamination. 20×Wash solution Store at 2~8°C for 1 month, avoiding contamination. Antibody/enzyme diluent Store at 2~8°C for 1 month, avoiding contamination. Substrate solution Store at 2~8°C for 1 month, avoiding light exposure. Stop solution Can be stored at room temperature. Plate seal film Can be stored at room temperature.                                    Documents: Manuals P162002-EN-Manual.pdf

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Human Interleukin-10 (Human IL-10), also known as Cytokine Synthesis Inhibitory Factor (CSIF), can inhibit the activation of Th1 cells and the production of related cytokines (such as IL-1β and TNF-α). IL-10 is a 178 amino acid molecule containing two intrachain disulfide bridges and is expressed as a non-covalently associated homodimer with a molecular weight of 36 kDa. Mature human IL-10 shares 72% to 86% amino acid sequence identity with IL-10 from cow, dog, horse, cat, mouse, sheep, pig, and rat. IL-10 can target various leukocytes, attenuating excessive immune responses. It affects three important functions of monocytes/macrophages: antigen presentation, release of immune mediators, and phagocytosis, representing its inhibition of all functions of monocytes/macrophages. Similar to IL-4 and IL-5, IL-10 is also involved in the development of autoimmune diseases. IL-10's presence can be observed in inflammatory bowel disease (IBD); it plays a protective role in chronic liver inflammation. The Arcegen Human IL-10 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay kit used for the quantitative measurement of IL-10 in serum, plasma, and cell culture supernatants. Specific anti-IL-10 antibodies are pre-coated on a high-affinity enzyme-linked immunosorbent assay plate. Standard samples and test samples are added to the wells of the plate, and after incubation, IL-10 present in the samples binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for incubation and binding, followed by washing. Then, a streptavidin-HRP enzyme conjugate is added for incubation and binding. After washing, aTMB color substrate is added for color development in the absence of light. The intensity of the color reaction is directly proportional to the concentration of IL-10 in the sample. The reaction is terminated by adding a stop solution, and the absorbance values are measured at 450 nm wavelength (reference wavelength 570-630 nm). Specification Item Number P162010S/P162010E Specification 48 T / 96 T Detection Range 62.5-4000pg/mL Detection Method Sandwich ELISA Detection Time 4.5 hours Sensitivity 27.5pg/mL Dilution Linearity 79-117% Recovery Rate 80-118% Intra-assay Variability 5.9% Intra-assay Variability 7.1% Components Component Number Component Name Storage Temperature P162004S P162004E P162010-A ELISA Plate 2~8℃ 48 T 96 T P162010-B Standard 2~8℃ 1 tube 2 tubes P162010-C Detection antibody 2~8℃ 120 μL 240 μL P162010-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL P162010-E Sample Dilution Buffer 2~8℃ 8 mL 15 mL P162010-F Antibody/Enzyme Dilution Buffer 2~8℃ 15 mL 30 mL P162010-G 20x Wash Buffer 2~8℃ 25 mL 50 mL P162010-H Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL P162010-I Stop Solution Room Temperature 5 mL 10 mL P162010-J Plate Sealant Film Room Temperature 3 pieces 5 pieces Storage The kit can be stored at 2~8°C or according to the storage conditions specified for each component to avoid contamination and repeated freeze-thaw cycles. Diluted working concentration reagents should be prepared as needed and discarded after use; they should not be reused. The shelf life is 1 year. Table 1 Reagent Storage Table After Initial Use  Component Name Storage Conditions ELISA Plate   Unused strips can be returned to the aluminum foil bag,tightly sealed,and stored at 2-8°C to avoid moisture absorption. Standard Use within 48 hours after dissolution,store at 2-8°C to avoid contamination. Detection Antibody Use within 48 hours after dilution,store at 2~8°C to avoid contamination. Enzyme Conjugate Use within 48 hours after dilution,store at 2~8°C to avoid contamination. Sample Dilution Buffer Store at 2~8°C for 1 month,avoiding contamination. Antibody/Enzyme Dilution Buffer Store at 2~8°C for 1 month,avoiding contamination. 20x Wash Buffer Store at 2~8°C for 1 month,avoiding contamination. Substrate Solution Store at 2~8°C for 1 month,avoiding light exposure. Stop Solution Can be stored at room temperature. Plate Sealant Film Can be stored at room temperature.                                    Documents: Manuals P162010-EN-Manual.pdf

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Human IL-15 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of Human IL-15 in serum, plasma, and cell culture supernatants. Specific anti-IL-15 antibodies are pre-coated onto high-affinity enzyme plates. Standards and samples are added to the wells of the enzyme plate, and after incubation, IL-15 present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for binding and incubated, followed by washing, and then the enzyme complex (Streptavidin-HRP) is added for binding and incubated. After washing, a colorimetric substrateTMB is added for color development, avoiding light. The intensity of the color reaction is proportional to the concentration of IL-15 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm). Interleukin-15 (IL-15), a widely expressed 14 kDa cytokine, plays a crucial role in numerous immune-related diseases. It shares approximately 97% and 73% sequence identity with IL-15 from primates and rodents, respectively. IL-15 is secreted by monocytes/macrophages (and some other cells), especially macrophages following viral infection. With similar biological functions to IL-2, IL-15 stimulates T cell activation and proliferation by sharing the γc receptor subunit with IL-2. The roles of IL-15 in autoimmune processes (such as rheumatoid arthritis) and malignancies (such as adult T cell leukemia) underscore its significance as a key immunoregulatory molecule, garnering considerable attention from researchers. Specification Item Number P162018S / P162018E Specification 48 T / 96 T Detection Range 3.9-250 pg/mL Detection Method Sandwich ELISA Detection Species Human Detection Time 4.5 hours Sensitivity 1.58 pg/mL Dilution Linearity 83 - 124% Recovery Rate 82 - 116% Intra-assay Variability 3.2% Intra-assay Variability 5.8% Components Component Number Component Name Storage Temperature P162018S P162018E P162018-A ELISA Plate 2~8℃ 48 T 96 T P162018-B Standard 2~8℃ 1 tube 2 tubes P162018-C Detection Antibody 2~8℃ 120 μL 240 μL P162018-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL P162018-E 5x Dilution Buffer 2~8℃ 8 mL 15 mL P162018-F 20x Wash Buffer 2~8℃ 25 mL 50 mL P162018-G Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL P162018-H Stop Solution Room Temperature 5 mL 10 mL P162018-I Plate Sealers Room Temperature 3 pieces 5 pieces Storage The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year. Table 1 Reagent Storage Table After First Use  Material Name Storage Conditions Plate   Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption. Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination. Detection Antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination. Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination. 5×Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination. 20×Wash Buffer Store at 2~8°C for 1 month, avoiding contamination. Substrate solution Store at 2~8°C for 1 month, avoiding light exposure. Stop Solution Can be stored at room temperature. Plate Sealers Can be stored at room temperature.                                    Documents: Manuals P162018-EN-Manual.pdf

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Human IL-21 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of IL-21 in serum, plasma, and cell culture supernatants. Specific anti-IL-21 antibodies are pre-coated onto high-affinity enzyme plates. Standards and samples are added to the wells of the enzyme plate, and after incubation, IL-21 present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for binding and incubated, followed by washing, and then the enzyme complex (Streptavidin-HRP) is added for binding and incubated. After washing, a colorimetric substrateTMB is added for color development, avoiding light. The intensity of the color reaction is proportional to the concentration of IL-21 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm). Human Interleukin-21 (IL-21) is a type I cytokine that regulates the functions of T cells, B cells, NK cells, and myeloid cells. IL-21 is produced by activated follicular helper T cells (Tfh), Th17 cells, and NKT cells. IL-21 derived from Tfh cells plays a crucial role in the development of humoral immunity through its autocrine effects on Tfh cells and paracrine effects on immunoglobulin maturation, plasma cell differentiation, and memory B cells. Mature human IL-21 consists of 133 amino acids and shares 63% and 61% sequence identity with mouse and rat IL-21, respectively. IL-21 is closely associated with clinical conditions such as cancer immunotherapy, viral infections, and allergies. It also inhibits skin hypersensitivity reactions by limiting the production of allergen-specific IgE and degranulation of mast cells. Specification Item Number P162019S / P162019E Specification 48 T / 96 T Detection Range 15.63-1000 pg/mL Detection Method Sandwich ELISA Detection Species Human Detection Time 4.5 hours Sensitivity 8.16 pg/mL Dilution Linearity 81 - 117% Recovery Rate 76 - 121% Intra-assay Variability 3.3% Intra-assay Variability 5.0% Components Component Number Component Name Storage Temperature P162019S P162019E P162019-A ELISA Plate 2~8℃ 48 T 96 T P162019-B Standard 2~8℃ 1 tube 2 tubes P162019-C Detection Antibody 2~8℃ 120 μL 240 μL P162019-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL P162019-E 5x Dilution Buffer 2~8℃ 8 mL 15 mL P162019-F 20x Wash Buffer 2~8℃ 25 mL 50 mL P162019-G Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL P162019-H Stop Solution Room Temperature 5 mL 10 mL P162019-I Plate Sealers Room Temperature 3 pieces 5 pieces Storage The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year. Table 1 Reagent Storage Table After Initial Use  Material Name Storage Conditions Plate   Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption. Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination. Detection Antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination. Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination. 5×Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination. 20×Wash Buffer Store at 2~8°C for 1 month, avoiding contamination. Substrate solution Store at 2~8°C for 1 month, avoiding light exposure. Stop Solution Can be stored at room temperature. Plate Sealers Can be stored at room temperature.                                    Documents: Manuals P162019-EN-Manual.pdf

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Interleukin-6 (IL-6) was initially identified as a B cell differentiation factor. It is now known to be a multifunctional cytokine that regulates immune responses, hematopoiesis, acute-phase reactions, and inflammation. It has three receptor binding sites, including one specific receptor binding site for IL-6R and two binding sites for gp130. IL-6 is a multifunctional, α-helical, 22-28 kDa glycoprotein that plays important roles in acute-phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. Human IL-6 shares 39% amino acid sequence identity with mouse and rat IL-6. It is a pleiotropic cytokine that not only affects the immune system but also acts on various physiological events in other biological systems and organs. It can also induce the growth of myeloma and plasma cell tumors. The Arcegen Human IL-6 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitatively measuring Human Interleukin 6 (Human IL-6) in serum and plasma. Specific anti-Human Interleukin 6 antibodies are precoated onto high-affinity enzyme plates. Standard samples and test samples are added to the enzyme plate wells and incubated. Human Interleukin 6 in the samples binds to the solid-phase antibodies. After washing away unbound substances, detection antibodies are added and incubated, followed by washing and addition of enzyme conjugate (Streptavidin-HRP) for incubation. After washing, TMB substrate solution is added for color development in the dark. The intensity of the color reaction is directly proportional to the concentration of Human Interleukin 6 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at a wavelength of 450 nm (with a reference wavelength of 570 - 630 nm). Specification Item Number P162011S / P162011E Specification 48 T / 96 T Detection Range 7.81-500 pg/mL Detection Method Sandwich ELISA Species Detected human Detection Time 4.5 hours Sensitivity 2.53 pg/mL Dilution Linearity 75 - 116% Recovery Rate 75 - 108% Intra-assay Variability 6.1% Inter-assay Variability 7.6% Components Component Number Component Name Storage Temperature P162011S P162011E P162011-A ELISA Plate 2~8℃ 48 T 96 T P162011-B Standard sample 2~8℃ 1 tube 2 tubes P162011-C Detection antibody 2~8℃ 120 μL 240 μL P162011-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL P162011-E 5× dilution buffer 2~8℃ 8 mL 15 mL P162011-F 20× wash buffer 2~8℃ 25 mL 50 mL P162011-G Substrate solution 2~8℃(Avoid Light) 8 mL 15 mL P162011-H Stop solution Room Temperature 5 mL 10 mL P162011-I Plate sealing film Room Temperature 3 pieces 5 pieces Storage The kit can be stored at 2~8°C, or according to the storage conditions of individual components to avoid contamination and repeated freeze-thaw cycles. Diluted reagents prepared to working concentration should be used immediately and discarded; they should not be reused. The shelf life is 1 year. Table 1 Reagent Storage Table After Initial Use  Component Names Storage Conditions ELISA Plate   Unused strips can be returned to the aluminum foil bag,tightly sealed,and stored at 2-8°C to avoid moisture absorption. Standard sample Use within 48 hours after dissolution,store at 2-8°C to avoid contamination. Detection antibody Use within 48 hours after dilution,store at 2~8°C to avoid contamination. Enzyme conjugate Use within 48 hours after dilution,store at 2~8°C to avoid contamination. 5× dilution buffer Store at 2~8°C for 1 month,avoiding contamination 20× wash buffer Store at 2~8°C for 1 month,avoiding contamination Substrate solution Store at 2~8°C for 1 month,avoiding light exposure. Substrate Solution Store at 2~8°C for 1 month,avoiding light exposure. Stop Solution Can be stored at room temperature Plate sealing film Can be stored at room temperature                                    Documents: Manuals P162011-EN-Manual.pdf

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Description IL-6 is similar to other interleukins in that it has a compact globular folding structure and is held in place by two disulfide bonds. The cysteine involved in forming these two disulfide bonds is highly conserved. IL-6, expressed by a variety of cells, regulates cell growth and differentiation in various tissues and is an important pro-inflammatory and immunomodulatory cytokine.    Product Properties  Synonyms Interleukin-6,BSF2,HSF,IFNB2 Uniprot No. P05231 Source Recombinant Human IL-6 Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Val30-Met212. Molecular Weight The protein has a predicted MW of 22.75 kDa. And it migrates as 25-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. Purity > 95% as determined by SDS-PAGE. Biological Activity The ED50 as determined by a cell proliferation assay using human TF-1 cells is 0.23-0.48 ng/mL, corresponding to a specific activity of > 2.0 × 106 IU/mg. Endotoxin < 1 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS. Reconstitution Centrifuge tubes before opening. Dissolve lyophilized protein with PBS. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.     Shipping and Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.    Cautions 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only.

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Human IL-8 ELISA Kit is an in vitro enzyme-linked immunosorbent assay kit used for the quantitative determination of Human IL-8 (Interleukin-8) in serum and cell culture supernatants. Specific antibodies against Human IL-8 are pre-coated on high-affinity enzyme plates. Standard samples and test samples are added to the wells of the enzyme plate and, after incubation, the IL-8 present in the samples binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added and incubated to bind. Following another wash, an enzyme conjugate (Streptavidin-HRP) is added and incubated to bind. After washing, aTMB chromogenic substrate is added for color development in the absence of light. The intensity of the color reaction is proportional to the concentration of IL-8 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm). IL-8, also known as CXCL8, is produced by macrophages and epithelial cells. It is a member of the α or CXC chemokine family and has a molecular weight of 8-9 kDa. IL-8 is a chemotactic factor that can attract neutrophils, eosinophils, and T cells, but not monocytes. It also plays a role in neutrophil activation and is released from several cell types in response to inflammatory stimuli. Specification Item Number P162022S / P162022E Specification 48 T / 96 T Detection Range 7.81-500 pg/mL Detection Method Sandwich ELISA Detection Species Human Detection Time 5 hours Sensitivity 0.382 pg/mL Dilution Linearity 83.1 - 109.8% Recovery Rate 73.7 - 112.4% Intra-assay Variability 8.4% Intra-assay Variability 7.0% Components Component Number Component Name Storage Temperature P162022S P162022E P162022-A ELISA Plate 2~8℃ 48 T 96 T P162022-B Standard 2~8℃ 1 tube 2 tubes P162022-C Detection Antibody 2~8℃ 60 μL 120 μL P162022-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL P162022-E 5x Dilution Buffer 2~8℃ 12 mL 25 mL P162022-F 20x Wash Buffer 2~8℃ 25 mL 50 mL P162022-G Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL P162022-H Stop Solution Room Temperature 5 mL 10 mL P162022-I Plate Sealers Room Temperature 3 pieces 5 pieces Storage The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year. Table 1 Reagent Storage Table After Initial Use  Material Name Storage Conditions Plate   Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption. Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination. Detection Antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination. Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination. 5×Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination. 20×Wash Buffer Store at 2~8°C for 1 month, avoiding contamination. Substrate solution Store at 2~8°C for 1 month, avoiding light exposure. Stop Solution Can be stored at room temperature. Plate sealing film Can be stored at room temperature.                                    Documents: Manuals P162022-EN-Manual.pdf

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Human MMP-9 ELISA Kit is an in vitro enzyme-linked immunosorbent assay kit used for the quantitative determination of Human Matrix Metalloproteinase-9 (MMP-9) in serum, plasma, and cell culture supernatants. Specific antibodies against Human MMP-9 are pre-coated on high-affinity enzyme plates. Standard samples and test samples are added to the wells of the enzyme plate and, after incubation, the MMP-9 present in the samples binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added and incubated to bind. Following another wash, an enzyme conjugate (Streptavidin-HRP) is added and incubated to bind. After washing, a TMB chromogenic substrate is added for color development in the absence of light. The intensity of the color reaction is proportional to the concentration of MMP-9 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm). MMP-9, also known as Gelatinase B, 92 kDa type IV collagenase, 92 kDa gelatinase, or type V collagenase, is a glycosylated proenzyme. MMP-9 degrades components of the extracellular matrix (ECM) with high activity against denatured collagen (gelatin). It cleaves natural collagens of types III, IV, V, and XI, as well as elastin, nidogen-1, and vitronectin. MMP-9 can also cleave various chemokines and growth factors (such as IL-1β, CXCL 8/IL-8, CXCL 7, CXCL 4, CXCL 1, latent transforming growth factor-β, membrane-bound tumor necrosis factor-α, vascular endothelial growth factor, and basic fibroblast growth factor), amyloid precursor protein, substance P, and myelin basic protein. This activity can either increase or decrease the biological activity of soluble factors and can also release them from their association with the ECM. Specification Item Number P162025S / P162025E Specification 48 T / 96 T Detection Range 31.25-2000 pg/mL Detection Method Sandwich ELISA Detection Species Human Detection Time 5 hours Sensitivity 7.44 pg/mL Dilution Linearity 76.7 -105.9 % Recovery Rate 76.1 - 119.2 % Intra-assay Variability 4.6 % Intra-assay Variability 5.1 % Components Component Number Component Name Storage Temperature P162025S P162025E P162025-A Plate 2~8℃ 48 T 96 T P162025-B Standard 2~8℃ 1 tube 2 tubes P162025-C Detection Antibody 2~8℃ 1 tube 2 tubes P162025-D Enzyme Conjugate 2~8℃(Avoid Light) 170 μL 350 μL P162025-E 5x Dilution Buffer 2~8℃ 12 mL 25 mL P162025-F 20x Wash Buffer 2~8℃ 25 mL 50 mL P162025-G Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL P162025-H Stop Solution   Room Temperature 5 mL 10 mL P162025-I Plate Sealers Room Temperature 3 pieces 5 pieces Storage The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year. Table 1 Reagent Storage Table After Initial Use  Material Name Storage Conditions Plate   Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption. Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination. Detection Antibody Use within 48 hours after dissolution, store at 2~8°C to avoid contamination. Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination. 5×Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination. 20×Wash Buffer Store at 2~8°C for 1 month, avoiding contamination. Substrate solution Store at 2~8°C for 1 month, avoiding light exposure. Stop Solution Can be stored at room temperature. Plate sealing film Can be stored at room temperature.                                    Documents: Manuals P162025-EN-Manual.pdf

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Mouse interferon-gamma (Mouse IFN-γ) is a soluble homodimeric cytokine and the sole member of the type II interferon family. It is mainly secreted by natural killer (NK) cells and natural killer T (NKT) cells, playing a crucial role in immune function. Interferon-gamma (IFN-γ) is essential for innate and adaptive immunity against viral, bacterial, and parasitic infections. It acts as a vital activator of macrophages and induces the expression of major histocompatibility complex class II (MHC II). Aberrant expression of IFN-γ is associated with many autoimmune and inflammatory diseases. Besides directly inhibiting viral replication, IFN-γ is important for immune stimulation and regulation. It can effectively inhibit cell proliferation and is used clinically to treat various conditions such as primary thrombocythemia, chronic myeloid leukemia, polycythemia vera, and primary myelofibrosis. The Arcegen Mouse IFN-γ ELISA assay kit is an in vitro enzyme-linked immunosorbent assay (ELISA) kit used for quantitative measurement of mouse interferon-gamma (Mouse IFN-γ) in serum and plasma. Specific anti-mouse interferon-gamma antibodies are precoated on a high-affinity enzyme-linked immunosorbent assay (ELISA) plate. Standard samples and test samples are added to the wells of the ELISA plate, and after incubation, mouse interferon-gamma present in the samples binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for incubation, followed by washing. Streptavidin-horseradish peroxidase (HRP) conjugate is then added for incubation. After another washing step, a colorimetric substrateTMB is added for color development. The intensity of the color reaction is proportional to the concentration of mouse interferon-gamma in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength range of 570 - 630 nm). Specification Item Number P162007S / P162007E Specification 48 T / 96 T Detection Range 31.25-2000 pg/mL Detection Method Sandwich ELISA Detection Time 4.5 hours Sensitivity 5.65 pg/mL Dilution Linearity 82 - 120% Recovery Rate 79 - 108% Intra-assay Variability 4.5% Intra-assay Variability 6.4% Components Component Number Component Name Storage Temperature P162007S P162007E P162007-A ELISA Plate 2~8℃ 48 T 96 T P162007-B Standard 2~8℃ 1 tube 2 tubes P162007-C Detection antibody 2~8℃ 120 μL 240 μL P162007-D Enzyme conjugate 2~8℃(Avoid Light) 30 μL 60 μL P162007-E Sample Dilution Buffer 2~8℃ 8 mL 15 mL P162007-F Antibody/Enzyme Dilution Buffer 2~8℃ 15 mL 30 mL P162007-G 20x Wash Buffer 2~8℃ 25 mL 50 mL P162007-H Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL P162007-I Stop Solution Room Temperature 5 mL 10 mL P162007-J Plate Sealant Film Room Temperature 3 pieces 5 pieces Storage The kit can be stored at 2~8°C or according to the storage conditions provided for each component to avoid contamination and repeated freeze-thaw cycles. Diluted working concentration reagents should be used immediately and discarded after use; they should not be reused. The shelf life is 1 year. Table 1 Reagent Storage Table After Initial Use  Component Name Storage Conditions ELISA Plate   Unused strips can be returned to the aluminum foil bag,tightly sealed,and stored at 2-8°C to avoid moisture absorption. Standard Use within 48 hours after dissolution,store at 2-8°C to avoid contamination. Detection Antibody Use within 48 hours after dilution,store at 2~8°C to avoid contamination. Enzyme Conjugate Use within 48 hours after dilution,store at 2~8°C to avoid contamination. Sample Dilution Buffer Store at 2~8°C for 1 month,avoiding contamination. Antibody/Enzyme Dilution Buffer Store at 2~8°C for 1 month,avoiding contamination. 20x Wash Buffer Store at 2~8°C for 1 month,avoiding contamination. Substrate Solution Store at 2~8°C for 1 month,avoiding light exposure. Stop Solution Can be stored at room temperature. Plate Sealant Film Can be stored at room temperature.                                    Documents: Manuals P162007-EN-Manual.pdf

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