Recombinant Mouse Cardiotrophin­1 (Mouse CT-1)_C230530

Description

Cardiotrophin1 (CT1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). CT-1 is a pleiotropic cytokine which is expressed in various tissues
including the adult heart, skeletal muscle, ovary, colon, prostate and fetal lung. Studies showed CT-1 which induces cardiac myocyte hypertrophy in vitro can bind to and activate the ILST/gp130 receptor. Rat CT1 encodes a 203 amino acid (a.a.) residue protein that lacks a hydrophobic signal peptide and it shares 94% a.a. and 79% a.a. sequence identity with human and murine CT-1.

 

Product Properties

Synonyms

CT-1CT1; CTF1
Accession
Q60753
GeneID
13019

Source

E.coli-derived Mouse CT-1, Met1-Ala203, with an N-terminal Met.

Molecular Weight

Approximately 21.5 kDa.
AA Sequence

MSQREGSLED HQTDSSISFL PHLEAKIRQT HNLARLLTKY AEQLLEEYVQ QQGEPFGLPG
FSPPRLPLAG LSGPAPSHAG LPVSERLRQD AAALSVLPAL LDAVRRRQAE LNPRAPRLLR
SLEDAARQVR ALGAAVETVL AALGAAARGP GPEPVTVATL FTANSTAGIF SAKVLGFHVC
GLYGEWVSRT EGDLGQLVPG GVA

Tag
None
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 95% by SDS-PAGE and HPLC analyses.
Biological Activity

The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.5 ng/ml,
corresponding to a specific activity of > 2.0 × 10 6 IU/mg. Fully biologically active when compared to
standard.

Endotoxin

Less than 1 EU/µg of rMuCT-1 as determined by LAL method.

Formulation

Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris, 300 mM NaCl, pH 8.5.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.
Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further
dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!

Recombinant Mouse Cardiotrophin­1 (Mouse CT-1)_C230530

Product form

SKU: C230530E

$179.00

    • Shipped today? Order within: Dec 26, 2024 17:00:00 -0500
    • Tell a unique detail about this product

    Description

    Cardiotrophin1 (CT1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). CT-1 is a pleiotropic cytokine which is expressed in various tissues
    including the adult heart, skeletal muscle, ovary, colon, prostate and fetal lung. Studies showed CT-1 which induces cardiac myocyte hypertrophy in vitro can bind to and activate the ILST/gp130 receptor. Rat CT1 encodes a 203 amino acid (a.a.) residue protein that lacks a hydrophobic signal peptide and it shares 94% a.a. and 79% a.a. sequence identity with human and murine CT-1.

     

    Product Properties

    Synonyms

    CT-1CT1; CTF1
    Accession
    Q60753
    GeneID
    13019

    Source

    E.coli-derived Mouse CT-1, Met1-Ala203, with an N-terminal Met.

    Molecular Weight

    Approximately 21.5 kDa.
    AA Sequence

    MSQREGSLED HQTDSSISFL PHLEAKIRQT HNLARLLTKY AEQLLEEYVQ QQGEPFGLPG
    FSPPRLPLAG LSGPAPSHAG LPVSERLRQD AAALSVLPAL LDAVRRRQAE LNPRAPRLLR
    SLEDAARQVR ALGAAVETVL AALGAAARGP GPEPVTVATL FTANSTAGIF SAKVLGFHVC
    GLYGEWVSRT EGDLGQLVPG GVA

    Tag
    None
    Physical Appearance
    Sterile Filtered White lyophilized (freeze-dried) powder.

    Purity

    > 95% by SDS-PAGE and HPLC analyses.
    Biological Activity

    The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.5 ng/ml,
    corresponding to a specific activity of > 2.0 × 10 6 IU/mg. Fully biologically active when compared to
    standard.

    Endotoxin

    Less than 1 EU/µg of rMuCT-1 as determined by LAL method.

    Formulation

    Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris, 300 mM NaCl, pH 8.5.

    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.
    Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further
    dilutions should be made in appropriate buffered solutions.

     

    Shipping and Storage

    The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Cautions

    1. Avoid repeated freeze-thaw cycles.
    2. For your safety and health, please wear lab coats and disposable gloves for operation.
    3. For research use only!

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