Recombinant Mouse B-cell Lymphoma-extra Large (Mouse Bcl-xL)_C230523

Description

Bcl-xL is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability. Bcl-xL is an anti- apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-xL is
present on the outer mitochondrial membrane and is also found on other membranes in some cell types. Natural Bcl-xL contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-xL missing the mitochondrial targeting sequence maintains its ability to neutralize pro- apoptotic Bcl-2 family members. Neutralization by Bcl-xL appears to be through binding the BH3 region of pro- apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.

 

Product Properties

Synonyms

Apoptosis regulator Bcl-X; bcl2-L-1
Accession
Q64373
GeneID
12048

Source

E.coli-derived Human Mouse Bcl-xL, Ser2-Arg212, with an N-terminal Met.

Molecular Weight

Approximately 23.7 kDa.
AA Sequence

SQSNRELVVD FLSYKLSQKG YSWSQFSDVE ENRTEAPEET EAERETPSAI NGNPSWHLAD
SPAVNGATGH SSSLDAREVI PMAAVKQALR EAGDEFELRY RRAFSDLTSQ LHITPGTAYQ
SFEQVVNELF RDGVNWGRIV AFFSFGGALC VESVDKEMQV LVSRIASWMA TYLNDHLEPW
IQENGGWDTF VDLYGNNAAA ESRKGQERFN R

Tag
None
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 97% by SDS-PAGE and HPLC analyses.
Biological Activity
Test in Process.

Endotoxin

Less than 0.1 EU/µg of rMuBcl-xL as determined by LAL method.

Formulation

Lyophilized from a 0.2 µm filtered concentrated solution in 2 × PBS, pH 7.4, 5% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.
Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further
dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!

Recombinant Mouse B-cell Lymphoma-extra Large (Mouse Bcl-xL)_C230523

Product form

SKU: C230523E

$179.00

    • Shipped today? Order within: Dec 26, 2024 17:00:00 -0500
    • Tell a unique detail about this product

    Description

    Bcl-xL is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability. Bcl-xL is an anti- apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-xL is
    present on the outer mitochondrial membrane and is also found on other membranes in some cell types. Natural Bcl-xL contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-xL missing the mitochondrial targeting sequence maintains its ability to neutralize pro- apoptotic Bcl-2 family members. Neutralization by Bcl-xL appears to be through binding the BH3 region of pro- apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.

     

    Product Properties

    Synonyms

    Apoptosis regulator Bcl-X; bcl2-L-1
    Accession
    Q64373
    GeneID
    12048

    Source

    E.coli-derived Human Mouse Bcl-xL, Ser2-Arg212, with an N-terminal Met.

    Molecular Weight

    Approximately 23.7 kDa.
    AA Sequence

    SQSNRELVVD FLSYKLSQKG YSWSQFSDVE ENRTEAPEET EAERETPSAI NGNPSWHLAD
    SPAVNGATGH SSSLDAREVI PMAAVKQALR EAGDEFELRY RRAFSDLTSQ LHITPGTAYQ
    SFEQVVNELF RDGVNWGRIV AFFSFGGALC VESVDKEMQV LVSRIASWMA TYLNDHLEPW
    IQENGGWDTF VDLYGNNAAA ESRKGQERFN R

    Tag
    None
    Physical Appearance
    Sterile Filtered White lyophilized (freeze-dried) powder.

    Purity

    > 97% by SDS-PAGE and HPLC analyses.
    Biological Activity
    Test in Process.

    Endotoxin

    Less than 0.1 EU/µg of rMuBcl-xL as determined by LAL method.

    Formulation

    Lyophilized from a 0.2 µm filtered concentrated solution in 2 × PBS, pH 7.4, 5% Trehalose.

    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.
    Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further
    dilutions should be made in appropriate buffered solutions.

     

    Shipping and Storage

    The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Cautions

    1. Avoid repeated freeze-thaw cycles.
    2. For your safety and health, please wear lab coats and disposable gloves for operation.
    3. For research use only!

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