Description
TL-1A belongs to the TNF superfamily of ligands. It is expressed predominantly in endothelial cells, and to a lesser extent in the placenta, lung, kidney, skeletal muscle, pancreas, small intestine and colon. TL-1A inhibits endothelial cell proliferation and angiogenesis.
Product Properties
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Synonyms
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TL1A; TNF-like 1
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Accession
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O95150
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Source
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E.coli-derived Human TL-1A protein,Leu72-Leu251.
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Molecular Weight
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Approximately 20.5 kDa
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Purity
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> 97% by SDS-PAGE.
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Endotoxin
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< 1.0 EU per 1μg of the protein by the LAL method.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4, with 1% BSA.
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Reconstitution
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1. We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile PBS, pH 7.4, with 0.1% BSA to a concentration of 0.1-1.0 mg/mL.
2. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. 3.Further dilutions should be made in PBS, which must contain carrier proteins, such as 0.1% BSA, 10% FBS, 5% HSA, 5% trehalose, one of four options.
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Shipping and Storage
The product should be stored at -25~-15℃ for 1 year date of receipt.
1 month, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ °C under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!
