Name: Recombinant Human Sonic Hedgehog N-Terminus (Human SHH)_C230493
Brand: Arcegen
SKU: C230493E
Price: 74.0 USD

Recombinant Human Sonic Hedgehog N-Terminus (Human SHH)_C230493

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SKU: C230493E

$74.00

Synonyms	HHG1, HHG-1, HLP3, HPE3, MCOPCB5
Accession	Q15465
Source	E.coli-derived Human shh, Cly25-Gly197, with an N-terminal Met.
Molecular Weight	Approximately 19.8kDa.
AA Sequence	IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEE SLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSVAAKSGG
Tag	None
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Purity	> 98 % by SDS-PAGE and HPLC analyses.
Biological Activity	The ED50 as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than 1 μg/mL, corresponding to a specific activity of > 1.0 × 10³ IU/mg. Fully biologically active when compared to standard.
Endotoxin	Less than 1.0 EU/μg of the protein as determined by LAL method.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

Synonyms	HHG1, HHG-1, HLP3, HPE3, MCOPCB5
Accession	Q15465
Source	E.coli-derived Human shh, Cly25-Gly197, with an N-terminal Met.
Molecular Weight	Approximately 19.8kDa.
AA Sequence	IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEE SLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSVAAKSGG
Tag	None
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Purity	> 98 % by SDS-PAGE and HPLC analyses.
Biological Activity	The ED50 as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than 1 μg/mL, corresponding to a specific activity of > 1.0 × 10³ IU/mg. Fully biologically active when compared to standard.
Endotoxin	Less than 1.0 EU/μg of the protein as determined by LAL method.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.