Description
CD40 Ligand, also known as TNFSF, a type II membrane protein with homology to TNF, is transiently expressed on activated T cells and known to be important for B cell Ig production and for activation and differentiation of monocytes and dendritic cells. Mature human CD40 Ligand consists of a 22 amino acid (aa) cytoplasmic domain, a transmembrane segment, and an 215 aa extracellular region . It is primarily expressed on activated CD4+ T lymphocytes, and also found in other types of cells, like NK cells, mast cells, basophils and eosinophils. In total CD40 ligand has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD40 Ligand dysregulation on T cells and antigen presenting cells contributes to the immune deficiency associated with HIV infection and AIDS. CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses.
Product Properties
| Synonyms |
CD154 antigen, CD154, CD40 antigen ligand, CD40 Ligand, gp39, HIGM1, T-B cell-activating molecule, T-BAM, TNFSF5, TNFSF5IMD3, TRAP
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Accession
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Source
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E.coli-derived human sCD40L protein, Met113-Leu261.
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Molecular Weight
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MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY
AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ
PGASVFVNVT DPSQVSHGTG FTSFGLLKL
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Tag
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Sterile Filtered White lyophilized (freeze-dried) powder.
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| Purity |
> 95 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 5-10 ng/mL. Fully biologically active when compared to standard.
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Endotoxin
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Less than 1.0 EU/μg of the protein as determined by LAL method.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 20mM PBS, pH 7.0.
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C.
Further dilutions should be made in appropriate buffered solutions.
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Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Caution
1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only.
