Description
Galectin-3, is classified as a chimeric member of the Galectin superfamily. Nuclear Galectin-3 can modulate gene expression, while cytosolic Galectin-3 can inhibit apoptosis. Galectin-3 contributes to the innate immune response against Candida albicans and Streptococcus pneumoniae, and it can facilitate acute inflammatory responses via neutrophil activation and opsonization, macrophage recruitment, and mast cell activation.
Product Properties
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Synonyms
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AGE-R3, CBP35, GAL3, L29, LGALS3, Mac-2
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Accession
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Source
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Yeast-derived Human Galectin-3, Ala2-Ile250.
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Molecular Weight
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Approximately 26.0 kDa.
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Tag
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None
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Purity
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> 90% by SDS-PAGE analyses.
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Biological Activity
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Recombinant human Galectin-3 is able to agglutinate red blood cells at a minimumeffective concentration of 25ug/ml.
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Endotoxin
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< 0.1 EU per 1μg of the protein by the LAL method.
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Formulation
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Lyophilized from a 0.2 µm filtered solution in 1 × PBS, pH 7.4, 1%BSA. |
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Reconstitution
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1. We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile PBS, pH 7.4, with 0.1% BSA to a concentration of 0.1-1.0 mg/mL.
2. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in PBS, which must contain carrier proteins, such as 0.1% BSA, 10% FBS, 5% HSA, 5% trehalose, one of four options.
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Shipping and Storage
The products are shipped with ice pack and can be stored at -25~-15℃ for 1 year.
1 months, 2 ~8℃ under sterile conditions after reconstitution.
3 months, -25 ~ -15℃ under sterile conditions after reconstitution.
Cautions
1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!
