Recombinant Human Fibroblast Growth Factor 21 (Human FGF-21)_C230344

Description

Fibroblast growth factor-21 (FGF-21) belongs to the large FGF family which is encoded by the FGF-21 gene. Mature human FGF-21 shows 81% aa identity to mouse FGF-21, and is known to be active on mouse cells. The c-DNA for FGF-21 predicts a 209 aa
polypeptide that contains a 28 aa signal sequence and a 181 aa mature region. FGF21 is a metabolic regulator that provides efficient and durable glycemic and lipid control in various animal models. And the FGF21 is a liver-derived endocrine factor that stimulates
glucose uptake in adipocytes. Pharmacologic studies show that FGF21 has broad metabolic actions in obese rodents and primates that include enhancing insulin sensitivity, decreasing triglyceride concentrations, and causing weight loss.

 

Product Properties

Synonyms

FGF21, FGF-21, fibroblast growth factor 2
Accession
Q9NSA1
GeneID
26291
Source
E.coli-derived human FGF-21 protein, His29-Ser209
Molecular Weight
Approximately 19.4 kDa.
AA Sequence
HPIPDSSPLL QFGGQVRQRY LYTDDAQQTE AHLEIREDGT VGGAADQSPE SLLQLKALKP GVIQILGVKT SRFLCQRPDG ALYGSLHFDP EACSFRELLL EDGYNVYQSE AHGLPLHLPG
NKSPHRDPAP RGPARFLPLP GLPPALPEPP GILAPQPPDV GSSDPLSMVG PSQGRSPSYA S
Tag
None
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
>96% by SDS-PAGE and HPLC analyses.
Biological Activity
The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/ml, corresponding to a specific activity of > 2.0 × 10 3 IU/mg in the presence of 5 µg/ml of recombinant mouse Klotho-β and 10 μg/ml of heparin. Fully biologically active when compared to standard.
Endotoxin
< 1.0 EU per 1μg of the protein by the LAL method.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!

Recombinant Human Fibroblast Growth Factor 21 (Human FGF-21)_C230344

Product form

SKU: C230344E

$74.00

    • Shipped today? Order within: Dec 26, 2024 17:00:00 -0500
    • Tell a unique detail about this product

    Description

    Fibroblast growth factor-21 (FGF-21) belongs to the large FGF family which is encoded by the FGF-21 gene. Mature human FGF-21 shows 81% aa identity to mouse FGF-21, and is known to be active on mouse cells. The c-DNA for FGF-21 predicts a 209 aa
    polypeptide that contains a 28 aa signal sequence and a 181 aa mature region. FGF21 is a metabolic regulator that provides efficient and durable glycemic and lipid control in various animal models. And the FGF21 is a liver-derived endocrine factor that stimulates
    glucose uptake in adipocytes. Pharmacologic studies show that FGF21 has broad metabolic actions in obese rodents and primates that include enhancing insulin sensitivity, decreasing triglyceride concentrations, and causing weight loss.

     

    Product Properties

    Synonyms

    FGF21, FGF-21, fibroblast growth factor 2
    Accession
    Q9NSA1
    GeneID
    26291
    Source
    E.coli-derived human FGF-21 protein, His29-Ser209
    Molecular Weight
    Approximately 19.4 kDa.
    AA Sequence
    HPIPDSSPLL QFGGQVRQRY LYTDDAQQTE AHLEIREDGT VGGAADQSPE SLLQLKALKP GVIQILGVKT SRFLCQRPDG ALYGSLHFDP EACSFRELLL EDGYNVYQSE AHGLPLHLPG
    NKSPHRDPAP RGPARFLPLP GLPPALPEPP GILAPQPPDV GSSDPLSMVG PSQGRSPSYA S
    Tag
    None
    Physical Appearance
    Sterile Filtered White lyophilized (freeze-dried) powder.
    Purity
    >96% by SDS-PAGE and HPLC analyses.
    Biological Activity
    The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/ml, corresponding to a specific activity of > 2.0 × 10 3 IU/mg in the presence of 5 µg/ml of recombinant mouse Klotho-β and 10 μg/ml of heparin. Fully biologically active when compared to standard.
    Endotoxin
    < 1.0 EU per 1μg of the protein by the LAL method.
    Formulation
    Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
    Reconstitution
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

     

    Shipping and Storage

    The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Cautions

    1. Avoid repeated freeze-thaw cycles.
    2. For your safety and health, please wear lab coats and disposable gloves for operation.
    3. For research use only!

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