Description
Bcl-w (also known as Bcl-2 like protein 2, BCL2L2) is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability. Bcl-w is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-w is required for normal sperm maturation. Natural Bcl-w contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-w missing the mitochondrial targeting sequence maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-w appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.
Product Properties
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Synonyms
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BCL2L2
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Source
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E.coli-derived Human Bcl-w, Ala2-Thr172.
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AA Sequence
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ATPASAPDTR ALVADFVGYK LRQKGYVCGA GPGEGPAADP LHQAMRAAGD EFETRFRRTF SDLAAQLHVT PGSAQQRFTQ VSDELFQGGP NWGRLVAFFV FGAALCAESV NKEMEPLVGQ
VQEWMVAYLE TQLADWIHSS GGWAEFTALY GDGALEEARR LREGNWASVR T
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Endotoxin
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< 1.0 EU per μg by the LAL method.
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Purity
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> 95% by SDS-PAGE and HPLC analyses.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in 25 mM Hepes, pH 7.4, 100 mM KCl, 10 % Glycerol, 5 % Trehalose, 0.02 % Tween-80.
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Applications
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ELISA, Kinetics (BLI), Kinetics (SPR), Immunization
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Dilution
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Dilute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL.
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Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Please operate with lab coats and disposable gloves,for your safety.
2. This product is for research use only.
