Description
Nerve growth factor (NGF) was composed of three non-covalently linked subunits, alpha, beta, and gamma. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity. NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system.
Product Properties
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Synonyms
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NGF; ngf; beta NGF; beta-NGF; HSAN5; NGFB
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Source
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HEK293 Cells-derived Human NGF protein, Met1-Ala241 with mutation Arg 121 Gly.
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Endotoxin
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< 1.0 EU per μg by the LAL method.
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Purity
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> 95% by SDS-PAGE analyses.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
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Applications
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ELISA, Kinetics (BLI), Kinetics (SPR), Immunization
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Dilution
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Dilute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL.
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Storage
The product should be stored at -25~-15℃ for 1 year from date of receipt.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Please operate with lab coats and disposable gloves,for your safety.
2. This product is for research use only.
