Description
The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assures fusion between virion membrane and endosomal membrane. In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein.
Product Properties
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Synonyms
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Middle surface antigen,PreS2.
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Source
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E.coli-derived HBsAg-preS2
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Endotoxin
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< 1.0 EU per μg by the LAL method.
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Purity
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> 95% by SDS-PAGE and HPLC analyses.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4, 50 mM NaCl.
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Applications
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ELISA, Kinetics (BLI), Kinetics (SPR), Immunization
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Dilution
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Dilute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL.
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Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Please operate with lab coats and disposable gloves,for your safety.
2. This product is for research use only.
