Description
Interleukin-3 is a pleiotrophic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. IL-3 exerts its biological activities via a
heterodimeric receptor composed of an IL-3 specific alpha chain and common beta chain that is shared with the IL-5 and GM-CSF high-affinity receptors. Receptors for IL-3 are present on bone marrow progenitors, and several mature myeloid cell types. Mature
canine IL-3 shares <40% amino acid sequence identity with IL-3 of other mammals.
Product Properties
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Synonyms
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Hematopoietic Growth Factor, MCGF, Multipotential Colony-stimulating Factor, P-cell-stimulating Factor
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E.coli-derived Canine IL-3, Arg24-Pro143.
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RPFSTDLPKQ YFTMINEIME MLNKSPSPSE EPLDSNEKET LLEDTLLRPN LDVFLNASSK FHKNGLLIWN NLKEFLPLLP TPTPRGEPIS IMENNWGDFQ RKLKKYLEAL DNFLNFKNKP
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Sterile Filtered White lyophilized (freeze-dried) powder.
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> 97% by SDS-PAGE and HPLC analyses.
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The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.2 ng/mL, corresponding to a specific activity of > 5.0 × 10 6 IU/mg. Fully biologically active when compared to
standard.
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< 1.0 EU per 1μg of the protein by the LAL method.
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Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
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Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!
