Recombinant Bovine Granulocyte Chemotactic Protein 2/CXCL6 (Bovine GCP-2/CXCL6)_C230264

Description

GCP-2 also known as CXCL6, is a CXC chemokine initially isolated as a neutrophil chemoattractant from the MG-63 osteosarcoma cell line. Among human CXC chemokines, GCP-2 is most closely related to ENA-78 (78% amino acid (aa) sequence identity in the mature peptide region and 86% identity in the signal sequence). The structure and sequence of the genes for human GCP-2 and ENA-78 also exhibit close similarity suggesting the two genes may have originated from a gene duplication. LIX (LPS-induced CXC chemokine) was initially cloned as a gene induced by LPS in mouse fibroblasts. The predicted LIX protein sequence is identical to a previously purified mouse protein designated mouse GCP-2 based on its amino sequence similarity (60% sequence identity) to human GCP-2. Mouse GCP-2/LIX is also 54% identical with human ENA-78 at the amino acid sequence level.

Product Properties

Synonyms
Chemokine alpha 3, CXCL6, GCP-2, CXCL6, member b
Accession
P80221
GeneID
281735
Source
E.coli-derived bovine GCP-2/CXCL6 protein, Gly37-Asn112
Molecular Weight
Approximately 8.0 kDa.
AA Sequence
GPVAAVVREL RCVCLTTTPG IHPKTVSDLQ VIAAGPQCSK VEVIATLKNG REVCLDPEAP LIKKIVQKIL DSGKNN
Tag
None
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
>97% by SDS-PAGE and HPLC analyses
Biological Activity
The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 10-50 ng/mL. Fully biologically active when compared to standard.
Endotoxin
< 0.1 EU per 1μg of the protein by the LAL method.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 500 mM NaCl, pH 7.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0
mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

Shipping and Storage

The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

Cautions

1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!

Recombinant Bovine Granulocyte Chemotactic Protein 2/CXCL6 (Bovine GCP-2/CXCL6)_C230264

Product form

SKU: C230264E

$155.00

      • Tell a unique detail about this product

      Description

      GCP-2 also known as CXCL6, is a CXC chemokine initially isolated as a neutrophil chemoattractant from the MG-63 osteosarcoma cell line. Among human CXC chemokines, GCP-2 is most closely related to ENA-78 (78% amino acid (aa) sequence identity in the mature peptide region and 86% identity in the signal sequence). The structure and sequence of the genes for human GCP-2 and ENA-78 also exhibit close similarity suggesting the two genes may have originated from a gene duplication. LIX (LPS-induced CXC chemokine) was initially cloned as a gene induced by LPS in mouse fibroblasts. The predicted LIX protein sequence is identical to a previously purified mouse protein designated mouse GCP-2 based on its amino sequence similarity (60% sequence identity) to human GCP-2. Mouse GCP-2/LIX is also 54% identical with human ENA-78 at the amino acid sequence level.

      Product Properties

      Synonyms
      Chemokine alpha 3, CXCL6, GCP-2, CXCL6, member b
      Accession
      P80221
      GeneID
      281735
      Source
      E.coli-derived bovine GCP-2/CXCL6 protein, Gly37-Asn112
      Molecular Weight
      Approximately 8.0 kDa.
      AA Sequence
      GPVAAVVREL RCVCLTTTPG IHPKTVSDLQ VIAAGPQCSK VEVIATLKNG REVCLDPEAP LIKKIVQKIL DSGKNN
      Tag
      None
      Physical Appearance
      Sterile Filtered White lyophilized (freeze-dried) powder.
      Purity
      >97% by SDS-PAGE and HPLC analyses
      Biological Activity
      The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 10-50 ng/mL. Fully biologically active when compared to standard.
      Endotoxin
      < 0.1 EU per 1μg of the protein by the LAL method.
      Formulation
      Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 500 mM NaCl, pH 7.0
      Reconstitution
      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0
      mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

      Shipping and Storage

      The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

      Cautions

      1. Avoid repeated freeze-thaw cycles.
      2. For your safety and health, please wear lab coats and disposable gloves for operation.
      3. For research use only!

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