Description
Human Interleukin-15 (IL-15) is a widely expressed 14 kDa cytokine that is plays an important role in many immunological diseases. It shares approximately 97% and 73% sequence identity with simian and murine IL-15, respectively. Both human and simian IL-15 are active on murine cells. IL-15 is secreted by mononuclear phagocytes (and some other cells), especially macrophages following infection by virus. IL-15 and IL-2 have similar biologic properties in vitro, consistent with their shared receptor (R) signaling components (IL-2/15Rβγc). A role for IL-15 during autoimmune processes such as rheumatoid arthritis and malignancies such as adult T-cell leukemia suggest that dysregulation of IL-15 may result in deleterious effects for the host.
Product Properties
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Synonyms
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Interleukin-15, IL-15, MGC972
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Source
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E.coli-derived human IL-15 protein, Asn49-Ser162.
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AA sequence
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NWVNVISDLK KIEDLIQSMH IDATLYTESD VHPSCKVTAM KCFLLELQVI SLESGDASIH DTVENLIILA NNSLSSNGNV TESGCKECEE LEEKNIKEFL QSFVHIVQMF INTS
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Endotoxin
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< 1.0 EU per μg by the LAL method.
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Purity
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> 97% by SDS-PAGE and HPLC analyses.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
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Applications
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ELISA, Kinetics (BLI), Kinetics (SPR), Immunization
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Dilution
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Dilute with sterile distilled water or aqueous buffer containing 0.1% BSA.
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Storage
The products are shipped with ice pack and can be stored at -20 ℃ for 1 year.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 °C under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Please operate with lab coats and disposable gloves,for your safety.
2. This product is for research use only.
