Human Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain. Human C5a has four alpha -helices plus three intrachain disulfide bonds that create a triple loop structure. In serum, proteolytic processing removes the Cterminal arginine, creating a low activity C5adesArg74 molecule. Human C5a is 60% and 54% aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a nonsignaling GPCR termed C5L2. Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation. It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.
Product Properties
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Synonyms
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CPAMD4
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Accession
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P01031
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GeneID
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727
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Source
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E.coli-derived Human C5a, Thr678-Arg751.
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Molecular Weight
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Approximately 8.3 kDa.
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AA Sequence
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TLQKKIEEIA AKYKHSVVKK CCYDGACVNN DETCEQRAAR ISLGPRCIKA FTECCVVASQ LRANISHKDM QLGR
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Tag
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None
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Purity
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> 98% by SDS-PAGE and HPLC analyses.
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Biological Activity
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The biological activity determined by its ability to chemoattract human monocytes using a concentration range of 1.0-10.0 ng/ml. Fully biologically active when compared to standard.
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Endotoxin
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< 1.0 EU per 1μg of the protein by the LAL method.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.
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Shipping and Storage
The products are shipped with ice pack and can be stored at -20 ℃ for 1 year.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 °C under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
