Description
BDNF is a member of the neurotrophin family of growth factors that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system.
Product Properties
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Synonyms
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Brain Derived Neurotrophic Factor, Abrineurin
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Uniprot No.
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P23560
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Source
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Recombinant Human BDNF Protein is expressed from CHO Cells Cells. It contains His129-Arg247.
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Molecular Weight
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12~14 kDa, on SDS-PAGE under reducing conditions.
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Biological Activity
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ED50 < 4.0 μg/ml, measured in a bioassay using C6 cells.
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Purity
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> 95% as determined by SDS-PAGE.
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Endotoxin
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<0.2 EU per 1μg of the protein by the LAL method.
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Formulation
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Lyophilized after extensive dialysis against PBS.
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Reconstitution
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It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH2O or PBS up to 100 μg/ml.
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Shipping and Storage
The product should be stored at -85~-65℃ for 6 months from date of receipt.
Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C.
For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
Cautions
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
