Description
Product description
D-luciferin is a common substrate for Luciferase and is widely used throughout biotechnology, especially in vivo imaging technology. Its mechanism of action involves oxidation and luminescence of luciferin (substrate) in the presence of ATP and luciferase enzyme. When luciferin is present in excess, the number of light photons produced is directly proportional to the concentration of luciferase enzyme (as illustrated in the diagram below). Plasmids carrying luciferase encoding gene (Luc) were transfected into cells and introduced into study animals such as rats and mice In vivo, Subsequent injection of luciferin allows for real-time monitoring of changes in light intensity using bioluminescence imaging (BLI), enabling the real-time monitoring of disease progression or the therapeutic efficacy of drugs. Additionally, the impact of ATP on this reaction system can be utilized, with changes in bioluminescence intensity indicating energy levels or vital signs.
D-luciferin is also commonly used in vitro for various research purposes, including analysis of luciferase activity and ATP levels, reporter gene assays, high-throughput sequencing, and various contamination detection assays.D-luciferin (free acid) has weak solubility in water and in buffered solutions unless dissolved in weak bases such as NaOH and KOH solutions. It is soluble in methanol (10 mg/mL) and DMSO (50 mg/mL). However, D-luciferin sodium salt and potassium salt forms can be easily and rapidly dissolved in water or buffered solutions, making them convenient to use. These solvents are non-toxic and particularly suitable for in vivo experiments. In most applications, there are no substantial differences among these three forms of D-luciferin once they are dissolved in solution.
Specifications
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English synonym
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(S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid sodium salt; D-Luciferin firefly, sodium salt monohydrate;
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CAS NO.
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103404-75-7
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Formula
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NaC11H7N2O3S2·H2O
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Molecular weight
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320.32 g/mol
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Appearance
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Light yellow powder
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Solubility
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Solube in water(100 mg/mL)
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Purity (HPLC)
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≥95%
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Components
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Components No.
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C331502E
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C331502S
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C331502M
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C331502L
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C331502T
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Size
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100 mg
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500 mg
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1 g
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5 g
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10 g
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Storage
Dry ice shipping. -20℃ storage, valid for one year.
