Arcegel Matrix for Organoid culture, Phenol Red-Free, LDEV-Free is a soluble basement membrane preparation extracted from EHS mouse tumors rich in extracellular matrix proteins. Its main components are laminin, type IV collagen, heparan sulfate proteoglycan (HSPG), nestin as well as growth factors such as TGF-beta, EGF, IGF, FGF, tissue plasminogen activator and other growth factors contained in EHS tumors. At room temperature, it aggregates to form a biologically active three-dimensional matrix, which simulates the structure, composition, physical properties and functions of the cell basement membrane in vivo, which is beneficial to the culture and differentiation of cells in vitro. It can be used for studies of cell morphology, biochemical function, migration, invasion and gene expression.
Arcegel Matrix is a sterile product, phenol red-free, LDEV-free, with a concentration of 8~12 mg/mL, and has been validated for organoid culture, meeting the requirements for organoid construction.
Feature
- High Safety: No LDEV (lactate dehydrogenase elevating virus)
- Multiple Concentration: the concentration range from 8 to 20 mg/ml
- Batch Consistency: Strict process through the whole product life cycle including the production, quality, warehouse etc.
- Low Endotoxin: Endotoxin content <8 EU/ml
- Contamination Control: Adhere to the rigorous residue quality standards of mycoplasmas, bacteria, and fungi
- High yield:＞30L per single batch
- Wide Compatibility: Compatible with almost all types of cell culture medium
Application
- 2D Culture Support
- Migration/Invasion
- Biochemical Research
- 3D & Organoid Culture
- Angiogenesis
- In Vivo Tumor Formation
Specification
| Concentration |
8~12 mg/mL
|
| Product Type |
Basement Membrane Matrix |
| Endotoxin Level |
Low |
| Transportation Conditions |
Dry Ice Transportation |
| Product Line |
Arcegel |
| Form |
Frozen |
| Phenol Red Indicator |
Contain |
| Product specifications |
5/10 mL |
| Species |
EHS Mouse Tumors |
| Classification |
Organoid Culture |
| Serum Level |
None |
| LDEV Detection |
None |
Components
| Components No. |
Name |
C231009E |
C231009S |
| C231009 |
Arcegel Matrix for Organoid culture, Phenol Red-Free, LDEV-Free |
5 mL |
10 mL |
Shipping and Storage
Transported on dry ice. Stored at -20°C with a shelf life of 2 years.
Figures
- Cell application validation
Figure 1. The application validation of Arcegel Matrix were as shown.
The representative image of HepG2 cell stained with crystal violet after invasion (Figure 1A). The image of 3D culture of HepG2 cell for 4 days (Figure 1B). The representative bright field and fluorescent images of HUVEC cell angiogenesis (Figure 1C)
Figure 2. Intestinal organoids formation
